EVOLUTE® HYDRO

The columns and 96-well plates contain the Hydro frit technology system, which reliably holds up sample and hydrolysis enzyme during incubation at elevated temperatures, and reduces clogging during subsequent clean up steps.

By combining Hydro frit technology with water wettable EVOLUTE sorbents, EVOLUTE HYDRO SPE products allow the hydrolyzed sample to be loaded directly onto the SPE bed for clean-up. Conditioning and equilibration steps are eliminated, again saving time and reducing costs.

Eliminating the need for off-line hydrolysis leads to significant time saving and reduction of sample handling, reducing  potential errors and sample losses. 

10 mg formats also allow analyte elution in very low volumes, allowing for no-blow down procedures.

Compared to traditional SPE procedures, EVOLUTE® HYDRO can reduce sample processing time by 20 minutes for 96 samples.

Automated extraction on Biotage® Extrahera™ using traditional SPE takes 53 minutes for 96 samples. With EVOLUTE® HYDRO this time is reduced to 33 minutes.
[30 mg 96-well plate format, using equivalent extraction methodology. Full procedure including hydrolysis, eluent evaporation and reconstitution: 143 mins (traditional SPE) 123 mins (HYDRO SPE)].

Because the HYDRO frit technology holds all of the sample above the sorbent bed during the hydrolysis process, hydrolysis efficiency when using EVOLUTE HYRO SPE columns and plates is equivalent to off-line hydrolysis under the same conditions. Existing hydrolysis procedures can easily be transferred to EVOLUTE HYDRO SPE products.

Whether extracting large multidrug panels or performing more targeted applications, EVOLUTE HYDRO SPE products remove matrix components and residual hydrolysis enzyme, leading to cleaner extracts and reduced matrix effects in LC-MS/MS.