Our ProPlus and ProPlus LX PhyTip columns are used for effective automated purification of IgG antibodies. ProPlus (MabSelect Sure™, Cytiva) and ProPlus LX (MabSelect™ SuRe™ LX, Cytiva) are affinity medias with an engineered version of the Protein A affinity ligand that demonstrate attributes of both Protein A and Protein G. The MabSelect™ SuRe™ medias favor an oriented binding of IgGs for high capacity binding of IgGs from low concentration samples and increased selectivity to mouse IgGs. In some cases, MabSelect™ SuRe™ demonstrates a 3-5 fold greater recovery compared to Protein G.
MabSelect™ SuRe™ is a licensed trademark of Cytiva.
PhyNexus, now part of Biotage, developed the PhyTip® column in 2002 and have supported researchers with automated protein purification ever since. PhyTip columns purify samples by repeated back-and-forth cycles - a process called dual flow chromatography which allows complete binding interactions in each purification step.
Due to maximization of binding of the sample, less protein is lost during the purification process. The result is very high sample recoveries.
Our unique column design with a packed resin bed results in binding of the entire sample to a small resin bed volume. The purified sample can be eluted in small volumes, resulting in a highly concentrated sample.
Effective purification by PhyTip columns enables a full purification protocol of 96 samples in as little as 15 minutes.
When choosing a PhyTip® column for your purification process, the optimal resin bed volume of the column depends on the total amount of protein present in your samples. If you are working with very low protein concentrations of 10 μg/mL or lower, we recommend using a resin bed volume one or two sizes bigger than indicated below for an effective purification process.
We offer PhyTip® columns available for all major liquid handling systems.
If you are not sure which PhyTip® column will that work best in your protocol, our specialists are here to help you.
Part No. | Description | Pack Size | List Price | |
---|---|---|---|---|
PTB-91-10-26 | ProPlus LX PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, Beckman. Incl. buffer kit. | 96 | 775 USD | Add to quote/cart |
PTX-91-10-26 | ProPlus LX PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, DD/Biotix | 96 | 775 USD | Add to quote/cart |
PTH-91-10-26 | ProPlus LX PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, Hamilton | 96 | 775 USD | Add to quote/cart |
PTR-91-10-26 | ProPlus LX PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, MEA2/ME | 96 | 775 USD | Add to quote/cart |
PTT-91-10-26 | ProPlus LX PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, Tecan | 96 | 775 USD | Add to quote/cart |
PTB-91-16-26 | ProPlus LX PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, Beckman | 96 | 2992 USD | Add to quote/cart |
PTX-91-16-26 | ProPlus LX PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, DD/Biotix | 96 | 2992 USD | Add to quote/cart |
PTH-91-16-26 | ProPlus LX PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, Hamilton | 96 | 2992 USD | Add to quote/cart |
PTR-91-16-26 | ProPlus LX PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, MEA2/ME | 96 | 2992 USD | Add to quote/cart |
PTT-91-16-26 | ProPlus LX PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, Tecan | 96 | 2992 USD | Add to quote/cart |
PTB-91-20-26 | ProPlus LX PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Beckman | 96 | 916 USD | Add to quote/cart |
PTX-91-20-26 | ProPlus LX PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, DD/Biotix | 96 | 916 USD | Add to quote/cart |
PTH-91-20-26 | ProPlus LX PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Hamilton | 96 | 916 USD | Add to quote/cart |
PTR-91-20-26 | ProPlus LX PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, MEA2/ME | 96 | 916 USD | Add to quote/cart |
PTT-91-20-26 | ProPlus LX PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Tecan | 96 | 916 USD | Add to quote/cart |
PTR-91-32-26 | ProPlus LX PhyTip® columns (1 mL volume) containing 320 µL affinity media per column, MEA2/ME | 96 | 3637 USD | Add to quote/cart |
PTB-91-40-26 | ProPlus LX PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, Beckman | 96 | 1331 USD | Add to quote/cart |
PTX-91-40-26 | ProPlus LX PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, DD/Biotix | 96 | 1331 USD | Add to quote/cart |
PTH-91-40-26 | ProPlus LX PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, Hamilton | 96 | 1331 USD | Add to quote/cart |
PTR-91-40-26 | ProPlus LX PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, MEA2/ME | 96 | 1331 USD | Add to quote/cart |
PTT-91-40-26 | ProPlus LX PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, Tecan | 96 | 1331 USD | Add to quote/cart |
PTB-91-80-26 | ProPlus LX PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Beckman | 96 | 2106 USD | Add to quote/cart |
PTX-91-80-26 | ProPlus LX PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, DD/Biotix | 96 | 2106 USD | Add to quote/cart |
PTH-91-80-26 | ProPlus LX PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Hamilton | 96 | 2106 USD | Add to quote/cart |
PTR-91-80-26 | ProPlus LX PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, MEA2/ME | 96 | 2106 USD | Add to quote/cart |
PTT-91-80-26 | ProPlus LX PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Tecan | 96 | 2106 USD | Add to quote/cart |
PTV-92-20-26 | ProPlus LX PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, Agilent | 96 | 916 USD | Add to quote/cart |
PTB-92-20-26 | ProPlus LX PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, Beckman | 96 | 916 USD | Add to quote/cart |
PTR-92-20-26 | ProPlus LX PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, MEA2/ME | 96 | 916 USD | Add to quote/cart |
PTT-92-20-26 | ProPlus LX PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, Tecan | 96 | 916 USD | Add to quote/cart |
PTB-92-05-26 | ProPlus LX PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, Beckman | 96 | 681 USD | Add to quote/cart |
PTR-92-05-26 | ProPlus LX PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, MEA2/ME | 96 | 681 USD | Add to quote/cart |
PTT-92-05-26 | ProPlus LX PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, Tecan | 96 | 681 USD | Add to quote/cart |
PTX-93-20-26 | ProPlus LX PhyTip® columns (300 µL volume) containing 20 µL affinity media per column, DD/Biotix | 96 | 916 USD | Add to quote/cart |
PTH-93-20-26 | ProPlus LX PhyTip® columns (300 µL volume) containing 20 µL affinity media per column, Hamilton | 96 | 916 USD | Add to quote/cart |
PTX-93-05-26 | ProPlus LX PhyTip® columns (300 µL volume) containing 5 µL affinity media per column, DD/Biotix | 96 | 681 USD | Add to quote/cart |
PTH-93-05-26 | ProPlus LX PhyTip® columns (300 µL volume) containing 5 µL affinity media per column, Hamilton | 96 | 681 USD | Add to quote/cart |
PTT-95-16-26 | ProPlus LX PhyTip® columns (500 µL volume) containing 160 µL affinity media per column, Tecan | 96 | 2992 USD | Add to quote/cart |
PTT-95-20-26 | ProPlus LX PhyTip® columns (500 µL volume) containing 20 µL affinity media per column, Tecan | 96 | 916 USD | Add to quote/cart |
PTT-95-40-26 | ProPlus LX PhyTip® columns (500 µL volume) containing 40 µL affinity media per column, Tecan | 96 | 1331 USD | Add to quote/cart |
PTT-95-80-26 | ProPlus LX PhyTip® columns (500 µL volume) containing 80 µL affinity media per column, Tecan | 96 | 2106 USD | Add to quote/cart |
PTB-91-10-07 | ProPlus PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, Beckman | 96 | 775 USD | Add to quote/cart |
PTX-91-10-07 | ProPlus PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, DD/Biotix | 96 | 775 USD | Add to quote/cart |
PTH-91-10-07 | ProPlus PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, Hamilton | 96 | 775 USD | Add to quote/cart |
PTR-91-10-07 | ProPlus PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, MEA2/ME | 96 | 775 USD | Add to quote/cart |
PTT-91-10-07 | ProPlus PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, Tecan | 96 | 775 USD | Add to quote/cart |
PTB-91-16-07 | ProPlus PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, Beckman | 96 | 2992 USD | Add to quote/cart |
PTX-91-16-07 | ProPlus PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, DD/Biotix | 96 | 2992 USD | Add to quote/cart |
PTH-91-16-07 | ProPlus PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, Hamilton | 96 | 2992 USD | Add to quote/cart |
PTR-91-16-07 | ProPlus PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, MEA2/ME | 96 | 2992 USD | Add to quote/cart |
PTT-91-16-07 | ProPlus PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, Tecan | 96 | 2992 USD | Add to quote/cart |
PTB-91-20-07 | ProPlus PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Beckman | 96 | 916 USD | Add to quote/cart |
PTX-91-20-07 | ProPlus PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, DD/Biotix | 96 | 916 USD | Add to quote/cart |
PTH-91-20-07 | ProPlus PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Hamilton | 96 | 916 USD | Add to quote/cart |
PTR-91-20-07 | ProPlus PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, MEA2/ME | 96 | 916 USD | Add to quote/cart |
PTT-91-20-07 | ProPlus PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Tecan | 96 | 916 USD | Add to quote/cart |
PTR-91-32-07 | ProPlus PhyTip® columns (1 mL volume) containing 320 µL affinity media per column, MEA2/ME | 96 | 3637 USD | Add to quote/cart |
PTB-91-40-07 | ProPlus PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, Beckman | 96 | 1331 USD | Add to quote/cart |
PTX-91-40-07 | ProPlus PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, DD/Biotix | 96 | 1331 USD | Add to quote/cart |
PTH-91-40-07 | ProPlus PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, Hamilton | 96 | 1331 USD | Add to quote/cart |
PTR-91-40-07 | ProPlus PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, MEA2/ME | 96 | 1331 USD | Add to quote/cart |
PTT-91-40-07 | ProPlus PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, Tecan | 96 | 1331 USD | Add to quote/cart |
PTB-91-80-07 | ProPlus PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Beckman | 96 | 2106 USD | Add to quote/cart |
PTX-91-80-07 | ProPlus PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, DD/Biotix | 96 | 2106 USD | Add to quote/cart |
PTH-91-80-07 | ProPlus PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Hamilton | 96 | 2106 USD | Add to quote/cart |
PTR-91-80-07 | ProPlus PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, MEA2/ME | 96 | 2106 USD | Add to quote/cart |
PTT-91-80-07 | ProPlus PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Tecan | 96 | 2106 USD | Add to quote/cart |
PTV-92-20-07 | ProPlus PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, Agilent Bravo | 96 | 916 USD | Add to quote/cart |
PTB-92-20-07 | ProPlus PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, Beckman | 96 | 916 USD | Add to quote/cart |
PTR-92-20-07 | ProPlus PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, MEA2/ME | 96 | 916 USD | Add to quote/cart |
PTT-92-20-07 | ProPlus PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, Tecan | 96 | 916 USD | Add to quote/cart |
PTB-92-05-07 | ProPlus PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, Beckman | 96 | 681 USD | Add to quote/cart |
PTR-92-05-07 | ProPlus PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, MEA2/ME | 96 | 681 USD | Add to quote/cart |
PTT-92-05-07 | ProPlus PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, Tecan | 96 | 681 USD | Add to quote/cart |
PTX-93-20-07 | ProPlus PhyTip® columns (300 µL volume) containing 20 µL affinity media per column, DD/Biotix | 96 | 916 USD | Add to quote/cart |
PTH-93-20-07 | ProPlus PhyTip® columns (300 µL volume) containing 20 µL affinity media per column, Hamilton | 96 | 916 USD | Add to quote/cart |
PTX-93-05-07 | ProPlus PhyTip® columns (300 µL volume) containing 5 µL affinity media per column, DD/Biotix | 96 | 681 USD | Add to quote/cart |
PTH-93-05-07 | ProPlus PhyTip® columns (300 µL volume) containing 5 µL affinity media per column, Hamilton | 96 | 681 USD | Add to quote/cart |
PTT-95-16-07 | ProPlus PhyTip® columns (500 µL volume) containing 160 µL affinity media per column, Tecan | 96 | 2992 USD | Add to quote/cart |
PTT-95-20-07 | ProPlus PhyTip® columns (500 µL volume) containing 20 µL affinity media per column, Tecan | 96 | 916 USD | Add to quote/cart |
PTT-95-40-07 | ProPlus PhyTip® columns (500 µL volume) containing 40 µL affinity media per column, Tecan | 96 | 1331 USD | Add to quote/cart |
PTT-95-80-07 | ProPlus PhyTip® columns (500 µL volume) containing 80 µL affinity media per column, Tecan | 96 | 2106 USD | Add to quote/cart |
Biotage is the Global Go-To Separations Company, supporting customers from drug discovery and development through to diagnostics and analytical testing with intelligent and sustainable workflow solutions. Our expertise and top-tier separation solutions play a key role in streamlining our customers’ workflows and improving their outcomes. Headquartered in Sweden, Biotage operates globally with 700 employees, serving over 80 countries. Our company is listed on NASDAQ Stockholm (BIOT).
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