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TLC Plates
Thin-Layer Chromatography Plates
TLC is a commonly used method development tool for flash purification. Product mixtures separated using TLC generally can be purified by flash chromatography using the identical solvent system.
» APPLICATION NOTES
Using TLC to Accurately Predict FLASH Purification Results
Thin-layer chromatography (TLC) is a commonly used method development tool for flash purification. Product mixtures separated using TLC generally can be purified by flash chromatography using the identical solvent system. The method transfer accuracy of TLC to flash is based on the TLC silica and flash silica properties. When identical, the equation 1/Rf = CV holds, where Rf is the retention factor of a compound separated by TLC, and CV is the number of column volumes required to elute a compound. Cartridge loading capacity is based on the difference in CV (DCV) between two adjacent compounds.
Tags: Application Notes
» MANUALS & USER GUIDES
Method Development in Flash Purification
Flash purification involves a simple liquid chromatography technique
» Method development uses TLC as a way of deciding the parameters for the
separation
» Isocratic separations are easiest to develop, but gradient separations are
more powerful
» Software in the Isolera helps with conversion of an isocratic separation to
a gradient
» It is possible with the Spektra software to run step gradients
» Loading options are dependent on the column type
» SNAP offers the most flexibility
» Care must be taken to choose the best loading option to get good
purifications
Tags: Manuals & User Guides
Biotage KP-NH TLC Plates Care and Use Guide
Biotage KP-NH TLC plates come in a 10 x 10 cm format. The glass has been pre-scored to provide four 2.5 x 10 cm plates when appropriately snapped.
Tags: Manuals & User Guides
» POSTERS
P204 Why is reversed phase flash chromatography use increasing
Flash Chromatography is the primary tool used by medicinal chemists for reaction mixture purification. Over the past 10 or so years, the use of reversed-phase flash chromatography for reaction mixture purification has increased dramatically due to both chromatographic and non-chromatographic reasons.
Tags: HPLC,
Pharma,
Posters
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News
07 November 2016How Much Can I Load on my Flash Cartridge?
Senior Technical Specialist Bob Bickler digs into the limiting factors for sample load in flash chromatography on the Flash Purification Blog. "A question I hear a lot from chemists ...
15 January 2016How to Develop Reversed-phase Flash Column Chromatography Methods
Method transfer from reversed phase TLC to reversed phase flash column chromatography can be very challenging. Simply put, the process is to create a gradient on an HPLC system and t...
21 August 2015How to Optimize TLC to Enhance Purification by Flash Chromatography
Learn how to optimize solvent ratios for ideal Rf (retention factor) values on TLC plates in this blog post on the Flash Purification Blog. Maximizing efficiency of flash chromatography ac...
30 June 2015Tutorial Video – How to Load Flash Chromatography Cartridges
Biotage® SNAP Ultra flash cartridges allow you to load your samples in seven different ways. No matter what you are purifying - there is always a solution to get the best possible separati...
27 May 2015Creating The Perfect Gradient for Flash Purification
With the advent of automated gradient flash chromatography, this technique is now mainstream in synthesis labs. No longer are chemists relegated to isocratic elution on glass columns with manual fr...
06 August 2014Difficult Fractionation of Lipids Made Easy with Isolera™ Dalton Flash Purification
Fatty acids and their esters are found in many biological substrates. Many of these fats are unhealthy (e.g. saturated fats) while others have been found to be beneficial (e.g. unsaturated, polyuns...
29 July 2014Free Shipping on EU Web Orders
Buy your Biotage consumables through our website and shipping is free For a limited time, free shipping is applied to Biotage consumables orders through the Biotage website*. Let Biotage make orde...
11 February 2013TLC-to-Step Gradient capability significantly reduces both purification time and solvent use
Uppsala, Sweden, 1st February, 2013 Biotage (STO: BIOT), a leading global supplier of solutions and technology for analytical, medicinal and peptide chemistry, has announced the launch of an updat...
TLC to flash method transfer accuracy is improved when TLC plates and flash cartridges with identical silica from Biotage are used. For synthetic chemists, the benefits of matched TLC plates and flash cartridges are better purification throughput, increased compound purity and yield, and reduced solvent cost.
CONSUMABLES
| Part No. | Description | Product type | Pack Size | Price | ||
|
TLC-0510-FI | KP-SIL TLC Plates Glass 5 x 10 cm, 50/cs | Consumables | 50 | Log in for price | |
| TLC-1010-FI | KP-SIL TLC Plates Glass 10 x 10 cm, 25/cs | Consumables | 25 | Log in for price | ||
| TLC-2575-FI | KP-SIL TLC Plates Glass 2.5 x 7.5 cm, 100/cs | Consumables | 100 | Log in for price | ||
| TLC-KPNH-0510-FI | KP-NH TLC PLates Glass 5 x 10 cm, 50/cs | Consumables | 50 | Log in for price | ||