This application note describes the extraction of 18 growth promoters (steroids, resorcylic acid lactones and stilbenes) using ISOLUTE® HYDRO DME+ prior to LC/MS analysis. LOQs are typically < 0.4 ng/mL, with a load of 75 μL of urine.
Tags: 16-ß-hydroxystanozolol, Application Notes, Column, Dienestrol, Diethylstilbestrol, English, Food, Growth Promoters, Hexestrol, Hormone, Hormones, LC-MS/MS, Methylboldenone, Methyltestosterone, Steroid, Steroid Hormones, Steroids, Taleranol, Urine (Cattle), Urine (Pig), Urine (Sheep), Urine (horse), Veterinary drugs, Zearalenone, Zeranol, ß-nortestosterone, ß-zearalenol, α-boldenone, α-nortestosterone, α-trenbolone, α-zearalenol, β-boldenone, β-trenbolone
This application note describes the extraction of 25 illicit and prescribed drugs from hydrolyzed urine using ISOLUTE® HYDRO DME+ Dual Mode Extraction plates prior to UPLC-MS/MS analysis.
Because of the enhanced sample clean up delivered by ISOLUTE HYDRO DME+ products, analyte sensitivity is significantly increased compared to traditional dilute and shoot (D&S) techniques, resulting in reduced limits of quantitation (LOQ)
Tags: 6-MAM, 7-Aminoclonazepam, 7-amino-flunitrazepam, 96-well plate, Amphetamine, Amphetamines, Application Notes, Benzodiazepines, Benzoylecgonine, Buprenorphine, Clinical, Cocaine, Cocaines, Codeine, EDDP, Ecgonine Methyl Ester EME, Ecgonine methyl ester, English, Flunitrazepam, Forensic, Gabapentin, Hydrocodone, Hydrolysed urine, Ketamine, LC-MS/MS, MDMA, Mephedrone, Methadone, Morphine, Norbuprenorphine, Opioid, Oxazepam, Oxymorphone, PCP, Phencyclidine (PCP), Pregabalin, Ritalinic acid, Urine, Zaleplon
New, novel benzodiazepines have become some of the most abused unique psychoactive substances seen today. In the United States, these compounds are not approved for prescription use. Therefore, the demand for testing of these drugs has rapidly increased, both in the United States and worldwide. Urine and whole blood are common matrices of choice for forensic laboratories as they can be easy to collect, and they provide relevant information regarding recent or active use of illicit materials.
Tags: 96-well plate, Forensic, LC-MS/MS, Posters, Synthetic Benzodiazepines, Urine, Whole blood
Dilute and shoot (D/S) is the most common form of sample preparation for the high throughput analysis of drugs of abuse (DoA)in urine. Although superior in extract cleanliness, solid phase extraction is sometimes deemed too complex, expensive and time consuming especially when matrix hydrolysis is required. This poster aims to demonstrate a simplified sample preparation workflow utilizing on-plate urine hydrolysis, organic solvent precipitation followed by flow through filtration and matrix component scavenging. ASMS, 2020.
Tags: 6-MAM, Amphetamine, Codeine, Drugs of Abuse, Ecgonine Methyl Ester EME, Forensic, Gabapentin, Hydrocodone, Ketamine, LC-MS/MS, MDMA, Mephedrone, Morphine, Oxymorphone, Posters, Pregabalin, Urine
This poster evaluates two “pass through” solid phase extraction techniques: (protein removal/phospholipid depletion (ISOLUTE PLD+) and dual mode extraction (ISOLUTE HYDRO DME+)) and supported liquid extraction (ISOLUTE SLE+) for a group of about 100 drugs of abuse compounds in whole blood.
Tags: 96-well plate, Anticonvulsant, Antipsychotics, Barbiturates, Benzodiazepines, Cannabinoid, Clinical, English, Forensic, Hallucinogen, LC-MS/MS, Opiates, Opioid, Posters, Stimulants, Whole blood
This poster compares various sample preparation techniques (mixed-mode SPE (EVOLUTE EXPRESS CX, phospholipid depletion (ISOLUTE PLD+), supported liquid extraction (ISOLUTE SLE+) and dual mode extraction (ISOLUTE HYDRO DME+) for extraction of a panel of 98 drugs of abuse from urine.
Tags: 96-well plate, Amphetamines, Anticonvulsant, Antipsychotics, Barbiturates, Benzodiazepines, Cannabinoid, Clinical, English, Fentanyls, Forensic, Hallucinogen, LC-MS/MS, Opiates, Opioid, Posters, Stimulants, z-drugs
Sample preparation is generally regarded as a pain point for many labs as it can be laborious, time consuming and relatively expensive. In a high throughput setting performing offline chemistry also leads to increases in sample turnaround time and reporting.
This poster evaluates simplified sample preparation workflows using optimized sample hold-up technology allowing chemistry in-situ. Reduced labour, extraction complexity, processing time, solvent usage and resultant instrument contamination and downtime can offset upfront costs associated with sample preparation.
Tags: 96-well plate, Clinical, Drugs of Abuse, English, Forensic, Hydrolysed urine, LC-MS/MS, Over the counter pharmaceuticals, Pharmaceuticals, Plasma, Posters, Urine
This poster evaluates the extraction of a range of drugs of abuse from hydrolyzed and nonhydrolyzed urine using a novel flow through scavenging product, ISOLUTE® HYDRO DME+. Matrix component removal in terms of creatinine and urea, salt residue, pigmentation associated with urobillin content and protein removal will be demonstrated.
Tags: 96-well plate, Barbiturates, Benzodiazepines, Clinical, Cocaines, Column, Drugs of Abuse, English, Forensic, LC-MS/MS, Opiates, Opioid, Posters
Product Note for ISOLUTE® HYDRO DME+ Dual Mode Extraction 96-well Plates and Columns
Tags: 96-well plate, Amphetamines, Barbiturates, Benzodiazepines, Cannabinoid, Clinical, Cocaines, Column, Drugs of Abuse, English, Forensic, Hydrolysed urine, LC-MS/MS, Opiates, Opioid, Pain Management, Product Notes, Urine
These two application notes describe alternative approaches to the extraction of a suite of regulated veterinary growth promoters (including steroids, stilbenes and resorcylic acid lactones) from a...24 April 2019
This application note demonstrates the applicability of our newest sample preparation consumable, ISOLUTE® HYDRO DME+ for in-situ hydrolysis and clean-up of urine samples, for the analysis of a...13 February 2018
ISOLUTE® HYDRO DME+ is a new product targeted at drugs of abuse and pain management panels in urine applications. This novel patent pending product quickly and easily removes matrix components ...