Sample Preparation
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- Automated Sample Preparation Instruments
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- ISOLUTE® FILTER+ High Performance Filtration Plates
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- ISOLUTE® HYDRO DME+ Dual Mode Extraction Products
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- ISOLUTE® SPE Columns and Plates
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- ISOLUTE® 101
- ISOLUTE® AL-A, AL-N & AL-B
- ISOLUTE® Accessories
- ISOLUTE® C18
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- ISOLUTE® CBA
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- ISOLUTE® ENV+
- ISOLUTE® ENV+ On-line SPE Cartridges
- ISOLUTE® EPH
- ISOLUTE® FL
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- ISOLUTE® HCX-3
- ISOLUTE® HCX-5
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- ISOLUTE® HM-N Columns
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- ISOLUTE® NH2
- ISOLUTE® Na2SO4/FL
- ISOLUTE® O&G
- ISOLUTE® PAH
- ISOLUTE® PE-AX
- ISOLUTE® PH
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- ISOLUTE® SI
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- EVOLUTE® SPE Columns and Plates
- AFFINILUTE™ MIP Columns
- Service Agreements - Europe
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- Discontinued Products
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- Biotage® Horizon 4790
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ISOLUTE® 101
SPE Columns
ISOLUTE® 101 is a non-polar polymeric (PS-DVB) phase for extraction of polar analytes from aqueous samples.
| Chemical description | Unmodified polystyrene-divinyl benzene copolymer |
| Sorbent type | Non-polar |
| Average particle size | 65 µm |
| Pore diameter | 100 Å |
» APPLICATION NOTES
Analysis of Perfluorinated Alkyl Acids Specified in EPA M537 and Beyond Using the Triple Quad LCMS-8045
Analysis of the perfluorinated alkyl acids listed in EPA Method 537 along with fluorotelomeric alcohols and perfluorinated sulfonates were extracted using SPE (using ISOLUTE 101 500 mg/ 6 mL SDVB SPE columns) and analyzed on the LCMS 8045. Minimum Detection Limits(MDL) ranged from 0.69 to 3.25 ppt. Extraction recoveries were greater than 80% for all compounds, with surrogate recoveries within 10% of the true value.
Tags: Application Notes,
Column,
Drinking water,
English,
Environmental,
LC-MS/MS,
Polyfluorinated acids
Extraction of Acidic, Neutral and Basic Compounds from Post Mortem samples using ISOLUTE® 101 Columns
This application note demonstrates an effective and efficient suported liquid extraction protocol for the clean up and concentration of a range of forensically significant opiates and their metabolites
ISOLUTE, unknown screening,
Tags: Acidic drugs,
Application Notes,
Basic drugs,
Column,
Forensic,
Naphyrone,
Post-Mortem fluids,
Post-Mortem tissue
SPE Method Development Recommendations for Extraction of Peptides from Aqueous Synthesis Mixtures
This method is suggested for the purification (de-salting) of aqueous peptide synthesis mixtures using ISOLUTE 101 non-polar columns. See General Comments for details on how this approach works.
Tags: Application Notes
SPE Method Development Recommendations for Extraction of Oligonucleotides from Aqueous Synthesis Mixtures
This method is recommended for the extraction of oligonucleotides from aqueous synthesis mixtures using a non-polar retention mechanism. See General Comments for more details on how this works.
Tags: Application Notes
SPE Method Development Recommendations for Toxicological Screening of Postmortem Samples
This method is suggested for the simultaneous extraction of acidic, neutral and basic compounds from postmortem tissues, blood and urine. The retention mechanism is hydrophobic.
Tags: Application Notes
» CATALOGS
Analytical Sample Preparation 2019 Catalog
In this catalog product information is displayed with easy-to-follow application details and supporting documentation. Ordering information is found following each product listing.
Tags: Catalogs
» MANUALS & USER GUIDES
QuickStart Guide to SPE
QuickStart Guide to SPE
Tags: English,
Manuals & User Guides
Step By Step Guide To SPE Method Development
The first decision that needs to be made is what the final analysis will be for the analyte. This will have an impact on the sample and cartridge size, as well as the final elution solvent. Gas chromatography offers higher sensitivity than HPLC, while HPLC is better suited for ionisable species and very high molecular weights. If LC-MS is available, minimal sample clean-up may be required.
Tags: Manuals & User Guides
» MATERIAL SAFETY DATA SHEETS
Safety Data Sheet ISOLUTE 101
Laboratory chemica
Not for therapeutic or diagnostic use
Tags: English,
Material Safety Data Sheets
» POSTERS
Tips & Tricks for Extracting Perfluorinated Compounds from Drinking Water Using Solid Phase Extraction
Perfluorinated compounds (PFCs) are a group of compounds that have been used in a wide array of industrial and household applications, including fabric protectors, non-stick coatings for cookware, coatings for food packaging and in some fire-fighting foams.
Their persistence in the environment allows them to accumulate in water sources, particularly those used for consumption.
This work will demonstrate the use of a cartridge-based extraction
system to extract and quantify up to 24 PFC compounds in drinking
water to highlight the challenges associated with this application.
APHL 2019
Tags: Column,
Drinking water,
English,
Environmental,
LC-MS/MS,
PFAA,
PFAAs,
Perfluorooctanesulfonic acid (PFOS),
Perfluorooctanoic acid (PFOA),
Polyfluorinated acids,
Posters,
US EPA Method 537
» PRODUCT NOTES
Method Development in Solid Phase Extraction using ISOLUTE® 101 SPE Columns for the Extraction of Aqueous Samples
This Chemistry Data Sheet includes specifics on the use of ISOLUTE 101 for the extraction of polar analytes from aqueous samples (page 1), ordering information (page 2) and a general discussion on non-polar sorbents (appendix page 3).
Tags: Product Notes
Search all documents »
News
13 August 2018New Findings presented at ASMS 2018
Biotage presented five new posters at the ASMS conference in San Diego this June. We've collected them all below for download. P177: Comparison of Sample Preparation Options for the Extr...
11 January 2018The 2018 Analytical Catalog is Here
The Biotage 2018 catalog of analytical sample preparation products for chemistry professionals is now available. This edition includes a comprehensive range of sample preparation consumables for bi...
17 July 2017Biotage Goes Direct in Italy
On July 1st, Biotage expanded its direct sales organization and opened the door to analytical customers in Italy. Account Manager Gianluca Pessoni is added to the Italian team, filling the ana...
28 June 2017Extraction of Tobacco-Specific Nitrosamines (TSNAs) from Urine Using ISOLUTE® SLE+ Prior to UPLC/MS/MS Analysis
This application note from Biotage Labs describes a method for extracting nitrosamines from urine. The ISOLUTE® SLE+ sample preparation method allows for a fast extraction with sufficient sampl...
15 June 2017Extraction of Amphetamines and Analogues from Hair Using ISOLUTE® SLE+ Prior to GC/MS Analysis
This application note describes the extraction of amphetamine and amphetamine-style compounds from hair matrix prior to GC/MS analysis following bead homogenization. This application note describe...
20 March 2017Automated Fractionation of Extractable Petroleum Hydrocarbons (EPH) in Soil
This application note describes the operating conditions for the automated fractionation of EPH into aliphatic and PAH fractions using the ISOLUTE® EPH column in conjunction with the Biotage&re...
20 February 2017Extraction of 49 Illicit Drugs of Abuse from Whole Blood Using ISOLUTE® SLE+
This application note describes the extraction of 49 drugs of abuse from whole blood, prior to UPLC-MS/MS analysis. An effective and efficient ISOLUTE SLE+ protocol optimized the for ...
05 January 20162016 Analytical Sample Preparation Catalog Released
Now available, the Biotage 2016 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...
11 August 2015Why Dilute and Shoot Ruins Your Results
Dilute and shoot (D&S) methods are promoted for use by analytical instrument vendors in clinical, forensic toxicology and bioanalytical laboratories. More sensitive LC-MS/MS systems make this s...
27 March 2015New Video – Thirty Years of Packing Flash Columns
Biotage, pioneers in flash chromatography, started packing columns with silica for flash purification 30 years ago. Some of the first employees are still with us today. In this video, site director...
08 January 20152015 Analytical Sample Preparation Catalog Released
Now available, the Biotage 2015 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...
29 July 2014Free Shipping on EU Web Orders
Buy your Biotage consumables through our website and shipping is free For a limited time, free shipping is applied to Biotage consumables orders through the Biotage website*. Let Biotage make orde...
Q&A
SPE column capacity
01 July 2013Question:
What is the maximum capacity of an SPE column?
Answer:
The capacity of a sorbent is defined as the total mass of strongly retained analyte that can be retained by a given mass of the sorbent under optimum conditions.
For most sorbents, this can range from 1-5% by weight. 100 mg of sorbent might retain 1-5 mg of analyte.
Ion exchange sorbents typically have a capacity of 0.3-1 meq/g.
Browse more questions and answers >
ISOLUTE® 101 is an unmodified polystyrene-divinylbenzene copolymer, for extraction of a range of polar analytes from aqueous samples using a non-polar retention mechanism.
No secondary interactions are present, so pure elution solvents can be used.
CONSUMABLES
| Part No. | Description | Product type | Pack Size | Price | ||
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101-0002-A | ISOLUTE® 101 25 mg/1 mL | Consumables | 100 | Log in for price | |
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101-0010-B | ISOLUTE® 101 100 mg/3 mL | Consumables | 50 | Log in for price | |
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101-0020-B | ISOLUTE® 101 200 mg/3 mL | Consumables | 50 | Log in for price | |
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101-0020-C | ISOLUTE® 101 200 mg/6 mL | Consumables | 30 | Log in for price | |
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101-0050-B | ISOLUTE® 101 500 mg/3 mL | Consumables | 50 | Log in for price | |
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101-0050-C | ISOLUTE® 101 500 mg/6 mL | Consumables | 30 | Log in for price | |