Sample Preparation
Horizon Food & Environmental Products
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Flash Purification
Work-up Products
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Service & Support
- Automated Sample Preparation Instruments
- Manual Sample Preparation & Accessories
- ISOLUTE® FILTER+ High Performance Filtration Plates
- ISOLUTE® PPT+ Protein Precipitation Plates
- ISOLUTE® PLD+ Protein and Phospholipid Removal Products
- ISOLUTE® HYDRO DME+ Dual Mode Extraction Products
- ISOLUTE® QuEChERS
- ISOLUTE® SLE+ Supported Liquid Extraction Products
- ISOLUTE® SPE Columns and Plates
-
- ISOLUTE® 101
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-
- Biotage® Sfär - Overview
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- Service Agreements - Europe
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- Discontinued Products
-
- Biotage® SNAP Bio
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EVOLUTE® HYDRO
Solid Phase Extraction Columns and Plates with In-Well Hydrolysis Capability
EVOLUTE® HYDRO solid phase extraction columns and 96-well plates combine robust, high quality EVOLUTE SPE sorbents with Hydro frit technology, enabling effective in-situ in-well hydrolysis of urine samples. Sample clean-up is performed in the same column or well, without the need to transfer samples, saving time and reducing risky sample handling steps.
» APPLICATION NOTES
An In-Well Hydrolysis Solid Phase Extraction Method Using EVOLUTE® HYDRO CX for 96 Licit and Illicit Drugs of Abuse from Urine Prior to UPLC-MS/MS
This application note describes the extraction of 96 licit and illicit drugs of abuse from urine prior to UPLC-MS/MS analysis using EVOLUTE® HYDRO CX 96-well plates.
EVOLUTE® HYDRO CX plates offer an efficient way to perform hydrolysis in the well of the extraction plate. This method provides high analyte recovery, reduced extraction time due to the elimination of a sample transfer step as well as the elimination of the column conditioning and equilibration steps, and a reduced risk for sample carryover or cross-contamination due to the elimination of the sample transfer step.
Tags: 6-acetyl codeine,
6-acetylmorphine (MAM),
7-Aminoclonazepam,
7-Aminoflunitrazepam,
96-well plate,
Alprazolam,
Amitriptyline,
Amphetamine,
Amphetamines,
Analgesics,
Application Notes,
Aripiprazole,
Atomoxetine,
Barbiturates,
Benzodiazepines,
Benzoylecgonine,
Buprenorphine,
Bupropion,
Buspirone,
Butalbital,
Carbamate hypnotic,
Carbamate muscle relaxant,
Carbamates,
Carbazepine,
Carisoprodol,
Chlordiazepoxide,
Chlorpheniramine,
Chlorpromazine,
Clinical,
Clomipramine,
Clonazepam,
Clonidine,
Clozapine,
Cocaethylene,
Cocaine,
Cocaines,
Codeine,
Cyclobenzaprine,
Desalkylflurazepam,
Designer stimulants,
Dextromethorphan,
Diazepam,
Dihydrocodeine (DHC),
Drugs of Abuse,
Duloxetine,
EDDP,
English,
Ethyl Sulfate (EtS),
Extrahera,
Fentanyl,
Fluoxetine,
Forensic,
Gabapentin,
Haloperidol,
Hydrocodone,
Hydrolysed urine,
Hydromorphone,
Hydroxyalprazolam,
Hydroxybupropion,
Hydroxymidazolam,
Hydroxytriazolam,
Imipramine,
Ketamine,
LC-MS/MS,
Lamotrigine,
Levetiracetam,
Lidocaine,
Lorazepam,
MDA,
MDEA,
MDMA,
Meperidine,
Meprobamate,
Methadone,
Methamphetamine,
Methaqualone,
Methcathinone,
Midazolam,
Morphine,
N-des-tapentadol,
N-desmethylclomipramine,
N-desmethyltapentadol,
Naloxone,
Norbuprenorphine,
Nordiazepam,
Norfentanyl,
Norhydrocodone,
Norketamine,
Normeperidine,
Noroxymorphone,
Norpropoxyphene,
Nortryptiline,
Opiates,
Opioid,
Opioid agonist,
Oxazepam,
Oxcarbazepine,
Oxycodone,
Oxymorphone,
Pain Management,
Pentobarbital,
Pharmaceuticals,
Phencyclidine (PCP),
Phenobarbital,
Pregabalin,
Quetiapine,
Ritalinic acid,
Secobarbital,
Stimulants,
THC-COOH,
Tapentadol,
Temazepam,
Topiramate,
Tramadol,
Triazolam,
Trimipramine,
Urine,
Venlafaxine,
Zolpidem,
Zolpidem-phenyl-4-COOH,
mCPP,
z-drugs
» MANUALS & USER GUIDES
EVOLUTE® EXPRESS User Guide
EVOLUTE® EXPRESS Solid Phase Extraction products
from Biotage are designed for all your sample
preparation challenges. Whether you are working in the field of bio-analysis during drug development, clinical or forensic
toxicology, or with the diversity of samples in food
safety and environmental applications, the tools and
methods used for sample preparation have a clear
impact on productivity. This user guide contains information about choosing the correct EVOLUTE EXPRESS product for your application and optimizing your methodology for best results.
Tags: 96-well plate,
Clinical,
Column,
English,
Environmental,
Food,
Forensic,
Manuals & User Guides,
On-line SPE,
Pharma
QuickStart Guide to SPE
QuickStart Guide to SPE
Tags: English,
Manuals & User Guides
» POSTERS
Evolution of Sample Preparation: Workflow Simplification Utilizing Sample Hold-up Technology in Forensic and Clinical Analyses
Sample preparation is generally regarded as a pain point for many labs as it can be laborious, time consuming and relatively expensive. In a high throughput setting performing offline chemistry also leads to increases in sample turnaround time and reporting.
This poster evaluates simplified sample preparation workflows using optimized sample hold-up technology allowing chemistry in-situ. Reduced labour, extraction complexity, processing time, solvent usage and resultant instrument contamination and downtime can offset upfront costs associated with sample preparation.
ASMS 2019
Tags: 96-well plate,
Clinical,
Drugs of Abuse,
English,
Forensic,
Hydrolysed urine,
LC-MS/MS,
Over the counter pharmaceuticals,
Pharmaceuticals,
Plasma,
Posters,
Urine
Better Hydrolysis and Increased Efficiency for Urine Drug Testing Using Mixed-Mode Solid Phase Extraction with In-well Hydrolysis
This poster presents a method for detection of approximately 100 drugs and metabolites in urine using mixed-mode polymeric cation exchange solid phase extraction (SPE).
Four different glucuronidase enzymes and several incubation times and temperatures were evaluated to determine optimum hydrolysis conditions for seven commonly detected glucuronide metabolites. Samples were subjected to offline hydrolysis and SPE (EVOLUTE® EXPRESS CX, Biotage, Charlotte, NC) and results compared to an SPE plate that incorporates in-well hydrolysis (EVOLUTE HYDRO CX, Biotage).
MSACL 2018, Palm Springs, CA
Tags: 96-well plate,
Clinical,
Drugs of Abuse,
English,
Forensic,
Hydrolysed urine,
LC-MS/MS,
Pain Management,
Posters,
Urine
Sample Preparation Strategies for Urine Panels with 50 or More Drugs and Metabolites Analyzed by LC-MS/MS
This poster evaluates 3 different sample preparation approaches (ISOLUTE SLE+, EVOLUTE EXPRESS ABN, EVOLUTE EXPRESS CX) for extraction of large multi-drug urine panels.
Each approach is assessed in terms of suitability for extraction of analytes with different different properties (pka, LogP etc).
ASMS 2017
Tags: 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH),
6-acetylmorphine (MAM),
7-Aminoclonazepam,
96-well plate,
Alprazolam,
Amitriptyline,
Amphetamine,
Amphetamines,
Analgesics,
Barbiturates,
Benzodiazepines,
Benzoylecgonine,
Buprenorphine,
Butalbital,
Cannabinoid,
Carbamate hypnotic,
Carbamate muscle relaxant,
Carisoprodol,
Chlordiazepoxide,
Clinical,
Clonazepam,
Cocaine,
Cocaines,
Codeine,
Diazepam,
Dihydrocodeine (DHC),
Drugs of Abuse,
EDDP,
English,
Fentanyl,
Forensic,
Gabapentin,
Hydrocodone,
Hydrolysed urine,
Hydromorphone,
Ketamine,
LC-MS/MS,
Lorazepam,
MDMA,
Meperidine,
Meprobamate,
Methadone,
Methamphetamine,
Morphine,
N-des-tapentadol,
Naloxone,
Norbuprenorphine,
Nordiazepam,
Norfentanyl,
Norhydrocodone,
Norketamine,
Normeperidine,
Nortryptiline,
Opiates,
Opioid,
Oxazepam,
Oxycodone,
Oxymorphone,
Pain Management,
Phencyclidine,
Phenobarbital,
Posters,
Pregabalin,
Ritalinic acid,
Secobarbital,
Tapentadol,
Temazepam,
Tramadol,
Urine,
Zolpidem,
Zolpidem-phenyl-4-COOH,
z-drugs,
α-OH alprazolam
Analysis of Commercially Available Beta-Glucuronidase Enzymes and Optimum Hydrolysis Conditions in Urine for Licit and Illicit Drugs Using In-well Hydrolysis
Most drugs, both licit and illicit, are excreted in urine as glucuronide conjugates. Hydrolysis using beta-glucuronidase converts the glucuronidated metabolites back to their “free” or non-conjugated
form, increasing sensitivity for LC-MS/MS analysis. Hydrolysis using red abalone, abalone, and recombinant beta-glucuronidase enzymes was evaluated using an in-well hydrolysis plate to determine which provided the most efficient hydrolysis of glucuronide metabolites without affecting overall recovery of nonconjugated compounds. A glucuronide control containing 8 glucuronide compounds was used to determine the extent of hydrolysis that occurred. A non-conjugated control containing 96 licit and illicit drugs of abuse was evaluated to determine if signal suppression occurred as a result of enzyme hydrolysis.
ASMS 2017
Tags: 96-well plate,
Clinical,
Codeine,
Drugs of Abuse,
English,
Forensic,
Hydrolysed urine,
Hydromorphone,
LC-MS/MS,
Lorazepam,
Morphine,
Norbuprenorphine,
Oxazepam,
Oxymorphone,
Pain Management,
Posters,
Urine
Method Development Strategies Using Polymer-based SPE for the Analysis of Peptides Prior to UPLC-MS/MS Analysis
This poster investigates the selectivity of polymer-based solid phase extraction sorbents and rapid extraction of clinical matrices, matrix interference reduction and improved analyte detection.
ASMS 2017
Tags: 96-well plate,
Clinical,
English,
Oxytocin,
Peptides,
Pharma,
Posters,
Serum,
Vasopressin
Sensitive and Fast Measurement of Aminoglycoside Antibiotics in Milk, Meat or Eggs by HILIC-MS/MS and Identifcation using MRM Spectrum Mode
This poster includes a simple Load-Wash-Elute solid phase extraction method using EVOLUTE EXPRESS WCX, for extraction of aminoglycoside antibiotics from a range of meat, milk and egg samples.
The sample preparation method is automated using Biotage Extrahera, with fast, sensitive analysis utilizing HILIC-MS/MS with MRM Spectrum Mode for ID confirmation (Shimadzu LCMS-8060).
ASMS 2017
Tags: 96-well plate,
Amikacin,
Aminoglycoside antibiotics,
Apramycin,
Dihydrostreptomycin,
Eggs,
English,
Extrahera,
Food,
Gentamicin,
Gentamicin C1,
Gentamicin C1a,
Gentamicin C2,
Hygromycin B,
Kanamycin,
LC-MS/MS,
Meat,
Milk,
Neomycin,
Netilmicin,
Paromomycin,
Posters,
Sisomycin,
Spectinomycin,
Streptomycin,
Tobramycin,
Veterinary drugs
Effective Strategies for Phospholipid Removal using Polymer-based Solid Phase Extraction (SPE) and LC-MS/MS Analysis
This poster focuses on the different strategies that can be employed when using EVOLUTE SPE sorbents (with both single and dual retention mechanisms) to reduce or eliminate residual phospholipids in sample extracts.
HPLC 2014
Tags: 96-well plate,
Clinical,
English,
Forensic,
LC-MS/MS,
Pharma,
Posters
Sample Preparation and Protein: A Comparison of Protein Removal with Various Sample Preparation Techniques using Gel Electrophoresis
This poster uses gel electrophoresis data to visually illustrate the amount of residual protein in extracts prepared using protein precipitation, supported liquid extraction and various silica and polymer based SPE approaches.
HPLC2014
Tags: Biological fluids,
English,
HPLC,
LC-MS/MS,
Plasma,
Posters
Fast and Clean Extraction of Peptide Mixture from Biological Matrices with no Dry Down Steps Using EVOLUTE® ABN and EVOLUTE® EXPRESS ABN Solid Phase Extraction Prior To Liquid Chromatography Tandem Mass Spectrometry
This poster describes the use of EVOLUTE® ABN and EVOLUTE® EXPRESS ABN for the fast and efficient extraction of a diverse mixture of peptides spiked into biological matrices.
Pittcon 2014
Tags: 96-well plate,
ACTH (1-24),
Angiotensin Fragment 1-7,
Angiotensin II,,
Clinical,
Elafin,
Endorphin,
English,
Glycoprotein myelin,
Helicobacter pylori (2-20),
LC-MS/MS,
Peptides,
Pharma,
Plasma,
Posters,
Serum,
Vasoactive Intestinal Fragment 1-12
Fast and Clean Extraction of Peptide Mixture from Biological Matrices with no Dry Down Steps Using EVOLUTE® ABN and EVOLUTE® EXPRESS ABN Solid Phase Extraction Prior To Liquid Chromatography Tandem Mass Spectrometry
This poster outlines a new method for the extraction of a mixture of peptides from plasma using resin based SPE. Initial method optimization was performed using EVOLUTE ABN before comparison to the streamlined approach without plate conditioning and equilibration using EVOLUTE EXPRESS ABN. Presented at EBF 2012.
EBF, EBF 2012, Peptide, ABN, SPE, Polymer, Polymeric,
Tags: ACTH (1-24),
Alpha-Endorphin,
Angiotensin Fragment 1-7,
Angiotensin II,,
Elafin,
Helicobacter pylori (2-20),
LC-MS/MS,
Myelin Glycoprotein fragment (35-55) rat,
Peptides,
Pharma,
Plasma,
Posters,
Vasoactive Intestinal Fragment 1-12
» PRODUCT NOTES
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PRODUCT VIDEOS
EVOLUTE® Hydro
News
14 September 2018New Application Note for Simultaneous Extraction of Aldosterone and Angiotensin I and II from Human Plasma
Plasma renin activity (PRA) determination is used in the diagnosis of primary aldosteronism, an important cause of secondary hypertension (high blood pressure). Concomitant measurement of both aldo...
13 August 2018New Findings presented at ASMS 2018
Biotage presented five new posters at the ASMS conference in San Diego this June. We've collected them all below for download. P177: Comparison of Sample Preparation Options for the Extr...
24 October 2017Extraction of Desmopressin from Serum Using EVOLUTE® EXPRESS WCX Prior to LC-MS/MS Analysis
This application note from Biotage describes a solid phase extraction (SPE) protocol for the extraction of the peptide desmopressin from human serum prior to LC-MS/MS analysis. Desmopressi...
05 July 2017Extraction of Plasma Catecholamines and Metanephrines Using EVOLUTE® EXPRESS WCX SPE Prior to LC-MS/MS Analysis
This application note describes a mixed-mode weak cation exchange SPE protocol for the extraction of three catecholamines (epinephrine, norepinephrine and dopamine) and three metanephrine metabolit...
29 May 2017Extraction of Polyfluorinated Compounds in Water Using EVOLUTE® EXPRESS WAX SPE Prior to LC-MS/MS Analysis
Polyfluorinated compounds (PFCs) such as PFOS (perfluorooctanesulfonic acid) and PFOA (perfluorooctanoic acid) are man-made fluorosurfactants. Due to their widespread industrial use and chemical st...
19 May 2017Extraction of Oxytocin and Vasopressin from Serum Using EVOLUTE® EXPRESS ABN Prior to LC-MS/MS Analysis
The method described in this application note achieves high reproducible extraction recoveries of the peptides oxytocin and vasopressin from serum using a fast, simple EVOLUTE® EXPRESS Load-Was...
20 April 2017Extraction of Vitamin B7 (Biotin) from Serum Using EVOLUTE® EXPRESS ABN
Vitamin B7 (Biotin) is a water soluble vitamin necessary for cell growth. The method described in this application note achieves high reproducible extraction recoveries of vitamin B7 from serum whi...
15 October 2015Extraction of Epinephrine, Norepinephrine and Dopamine from Human Plasma
A new application note from Biotage describes an optimized method for extraction of the catecholamines dopamine, epinephrine and norepinephrine from human plasma. The extraction method utilizes the...
27 March 2015New Video – Thirty Years of Packing Flash Columns
Biotage, pioneers in flash chromatography, started packing columns with silica for flash purification 30 years ago. Some of the first employees are still with us today. In this video, site director...
29 July 2014Free Shipping on EU Web Orders
Buy your Biotage consumables through our website and shipping is free For a limited time, free shipping is applied to Biotage consumables orders through the Biotage website*. Let Biotage make orde...
10 July 2014New Posters by Biotage at ASMS 2014, Baltimore, MD, USA
Eight new posters by Biotage Scientists and collaborating groups have been presented at ASMS 2014, Baltimore, MD, USA. P81 A Reduced Workflow Method For The Extraction Of Vitamin B7 (bi...
30 June 2014EPRW (European Pesticides and Residues Workshop) 2014, Dublin
Biotage is attending EPRW (European Pesticides and Residues Workshop) 2014 30th June - 3rd July 2014, Dublin, Ireland Read more at conference website http://eprw2014.com/
20 May 2014New Posters by Biotage R&D Presented at HPLC 2014
Four new posters by Biotage R&D and Application Scientists have been presented this week at HPLC 2014, New Orleans, LA, USA. The studies focus on clean-up of biofluids using different sa...
01 April 2014Annual Meeting of the German Society for Experimental and Clinical Pharmacology and Toxicology (DGPT), Hannover
Biotage is attending the Annual Meeting of the German Society for Experimental and Clinical Pharmacology and Toxicology (DGPT) 1st - 3rd April 2014, Hannover, Germany Read more at the conference ...
11 January 201422nd Congress French Toxicology Society 19th Meeting Society of Hair Testing Annual Meeting Belgian and Luxembourg Toxicological Society, Bordeuax
Biotage is attending the 22nd Congress French Toxicology Society19th Meeting Society of Hair Testing Annual Meeting Belgian and Luxembourg Toxicological Society 11th-13th June 2014, Bordeuax, Fran...
28 August 2012Biotage announces EVOLUTE® EXPRESS 96-well plates
Uppsala, Sweden, 28th August, 2012 Biotage® (STO: BIOT), a leading global supplier of solutions and technology for analytical and medicinal chemistry, introduced EVOLUTE® EXPRESS, a novel ...
28 August 2012Biotage unveils EVOLUTE® EXPRESS
EVOLUTE EXPRESS 96-Well SPE EVOLUTE EXPRESS® represents a leap forward in high throughput solid phase extraction. The EVOLUTE EXPRESS range of 96-well SPE plates combine powerful EVOLUTE sorbe...
18 April 2011Biotage® Introduces EVOLUTE® WAX, Providing a Total Solution for Acidic Compounds
Uppsala, Sweden – Biotage, a leading supplier of tools and technology for medicinal and analytical chemistry, today announced the further expansion of its successful family of sample preparat...
16 December 2010Biotage introduces a new sample preparation application note for the extraction of ethyl glucuronide from urine by LC-MS
Biotage Announces an Easy, Cost-Effective Approach to the Analysis of Ethyl Glucuronide (EtG) in Urine for Clinical and Forensic Toxicology Laboratories Uppsala, Sweden – Biotage®...
23 February 2010Biotage Introduces EVOLUTE® WCX: Now Providing a Total Solution for Basic Compounds
Uppsala, Sweden, March 2, 2010– Biotage, a leading supplier of tools and technology for medicinal and analytical chemistry, today announced the further expansion of its successful family of p...
Q&A
Expiry date on ISOLUTE and EVOLUTE
01 July 2013Question:
Is there an expiry (‘best before’) date for ISOLUTE and EVOLUTE sample preparation products?
Answer:
There is no fixed expiry date for these products.
All ISOLUTE and EVOLUTE products are packaged in inner metal laminate barrier bags, which provide an effective barrier to oxygen and moisture penetration. Recommended storage conditions are standard (see below).
Recommended storage conditions
- Always store products sealed inside metal laminate barrier bags.
- Store products at room temperature, away from direct sunlight.
- Ensure products are not in contact with moisture, direct heat etc.
Non-bonded phases (e.g. Si, Fl, Al-A/N/B)
We have no indications that the performance of non-bonded phase SPE sorbents is affected by long term storage, under recommended conditions.
Bonded phases (e.g. C18, SCX, DIOL)
We have no indications that the performance of bonded phase SPE sorbents is affected by long term storage, under recommended conditions.
Browse more questions and answers >Streamlined Sample Processing
The columns and 96-well plates contain the Hydro frit technology system, which reliably holds up sample and hydrolysis enzyme during incubation at elevated temperatures, and reduces clogging during subsequent clean up steps.
By combining Hydro frit technology with water wettable EVOLUTE sorbents, EVOLUTE HYDRO SPE products allow the hydrolyzed sample to be loaded directly onto the SPE bed for clean-up. Conditioning and equilibration steps are eliminated, again saving time and reducing costs.
Eliminating the need for off-line hydrolysis leads to significant time saving and reduction of sample handling, reducing potential errors and sample losses.
10 mg formats also allow analyte elution in very low volumes, allowing for no-blow down procedures.
Reduced Processing Time
Compared to traditional SPE procedures, EVOLUTE® HYDRO can reduce sample processing time by 20 minutes for 96 samples.
Automated extraction on Biotage® Extrahera™ using traditional SPE takes 53 minutes for 96 samples. With EVOLUTE® HYDRO this time is reduced to 33 minutes.
[30 mg 96-well plate format, using equivalent extraction methodology. Full procedure including hydrolysis, eluent evaporation and reconstitution: 143 mins (traditional SPE) 123 mins (HYDRO SPE)].
No Compromise on Hydrolysis Efficiency
Because the HYDRO frit technology holds all of the sample above the sorbent bed during the hydrolysis process, hydrolysis efficiency when using EVOLUTE HYRO SPE columns and plates is equivalent to off-line hydrolysis under the same conditions. Existing hydrolysis procedures can easily be transferred to EVOLUTE HYDRO SPE products.
Excellent Sample Clean-up
Whether extracting large multidrug panels or performing more targeted applications, EVOLUTE HYDRO SPE products remove matrix components and residual hydrolysis enzyme, leading to cleaner extracts and reduced matrix effects in LC-MS/MS.
