EVOLUTE® CX

Malachite green (MG) and crystal (gentian) violet (CV) are triphenylmethane dyes used in aquaculture as fungicides and ectoparasiticides. They are rapidly metabolised to the leucomalachite green (LMG) and leucocrystal (gentian) violet (LCV) metabolites that persist in fish tissue. MG and CV are banned in many countries due to their mutagenicity and carcinogenicity, but may still be used illegally. Monitoring methodologies must be able to detect the compounds at 1 ng/g (US) or 2 ng/g (EU). In order to meet these LODs for complex matrices such as oily fish, and the low UV absorbance of the leuco forms, LC-MS-MS is the analytical method of choice. This application note focuses on ease of use when compared to other methods, and describes a QuEChERS based approach incorporating the use of aluminium oxide in enhanced dispersive SPE to reduce the levels of fat in the final extract. Additional cleanup is provided by polymeric EVOLUTE SPE columns, and the resulting extract demonstrated low ion suppression. Analyte recoveries of 60-105%, with low rsds, are achieved. The method is also applicable to Brilliant green. Malachite green, Leuco malachite green, Crystal violet, Leuco crystal violet, Brilliant Green, EVOLUTE, EVOLUTE CX, QuEChERS, LC/MS/MS, Salmon, Contamination, Gentian violet, SPE, Solid Phase Extraction, Polymer, Resin,
Tags: Application Notes, Brilliant Green, Column, Crystal Violet, Fish, Food, Food, Gentian Violet, LC-MS/MS, Leuco Crystal Violet, Leuco Malachite Green, Malachite Green, Salmon, Triphenylmethane Dyes, Veterinary drugs

The use of schedule I drugs for patient pain management therapy warrants constant monitoring of therapeutic levels in the patient. Screening patient urine samples for the free drugs is complicated by the metabolism process which converts the free drug to the -glucuronide form. Patient urine samples can be enzymatically hydrolyzed to cleave glucuronide moiety and produce the free form of drug. The target analytes can them be extracted from the urine matrix using EVOLUTE CX solid phase extraction cartridges. LOQs range from 5 ng/mL to 0.5 ng/mL with recoveries above 80% and RSD <10% Pain Management, EVOLUTE, CX, SPE, Solid Phase Extraction, Polymer, LC-MS-MS, Cocaine, Diazepam, Heroin, Morphine, Oxycodone, Fentanyl, Diazepam, Mixed Mode, RapidTrace
Tags: Alprazolam, Application Notes, Buprenorphine, Butylone, Clinical, Clonazepam, Codeine, Column, Fentanyl, Flunitrazepam, Hydrocodone, Hydromorphone, LC-MS/MS, Meperidine, Methadone, Morphine, Naloxone, Naltrexone, Oxycodone, Oxymorphone, RapidTrace, Temazepam, Urine

This method demonstrates the extraction of Mephedrone (MCAT) from a range of biological fluids including plasma, urine and whole blood. This extraction is achieved through cation exchange using EVOLUTE CX, a mixed-mode resin-based SPE sorbent. Analytes were quantified using LC-MS/MS and recoveries were all above 90%. SPE, ION EXCHANGE, EVOLUTE, CX, STRATA X, OASIS, DOA
Tags: Application Notes, Biological fluids, Forensic, LC-MS/MS, Mephedrone, Plasma, Urine, Whole blood

This application note describes the extraction of melamine from biological fluids using EVOLUTE CX. Sustained melamine exposure can result in kidney stones and renal failure, with the young being most susceptible. food scare, Melamine, China, kidney failure, renal failure, protein, Biotage, EVOLUTE,
Tags: Application Notes, Biological fluids, Column, Food, Food contaminant, LC-MS/MS, Melamine

This application note describes the extraction of melamine from biological fluids using EVOLUTE CX. Melamine's low cost and high nitrogen content has led to exploitation in various sections of the food industry, most notably involving dairy products. Biotage, cation exchange, oasis, food scare, china,
Tags: Application Notes, Column, Food, Food contaminant, LC-MS/MS, Melamine, Milk

This procedure is recommended for the extraction of cocaine and its principle metabolites (norcocaine, benzoylecgonine, ecgonine methyl ester, anhydroecgonine methyl ester and cocaethylene) from whole blood, plasma and urine using polymeric mixed-mode cation exchange SPE columns.
Tags: Anhydroecgonine methyl ester, Application Notes, Benzoylecgonine, Biological fluids, Butylone, Cocaines, Column, Ecgonine methyl ester, Forensic, LC-MS/MS, Norcocaine

This Application Note describes the extraction of a variety of basic drugs from plasma using EVOLUTE® CX mixed-mode resin-based SPE. The analyte suite includes basic drugs with wide ranging pKa and logP values. Basic Drugs, Biotage, Ion Exchange, EVOLUTE, Pharmaceuticals
Tags: Amitriptyline, Application Notes, Atenolol, Basic, Clinical, Fluoxetine, LC-MS/MS, Metoprolol, Mianserin, Naltrexone, Plasma, Procainamide, Quinidine, Ranitidine, Salbutamol

Laboratory chemical Not for therapeutic or diagnostic use
Tags: English, Material Safety Data Sheets

Tags: Material Safety Data Sheets

This poster compares the use of anion and cation exchange SPE approaches for the extraction of these important clinical biomarkers. MSACL Europe 2014
Tags: 96-well plate, Clinical, English, LC-MS/MS, Plasma, Posters, Reverse Tri-iodothyronine (rT3), Thyroid Hormone, Thyroxine (T4), Tri-iodothyronine (T3), rT3

This poster evaluates the performance of various polymer-based ion exchange SPE chemistries for the extraction of thyroid hormones prior to LC-MS/MS analysis.
Tags: 96-well plate, Clinical, English, LC-MS/MS, Posters, Reverse Tri-iodothyronine (rT3), Serum, T3, T4, Thyroid Hormone, Thyroxine (T4), Tri-iodothyronine (T3), rT3

This poster compares two different procedures for quantification of drugs of abuse from postmortem brain tissue.
Tags: 7-Aminoflunitrazepam, Amphetamine, Benzoylecgonine, Brain, Cocaine, Codeine, Column, Diazepam, Drugs of Abuse, English, Forensic, GC-MS, LC-MS/MS, Methadone, Morphine, Posters

This poster describes the extraction of a range of drugs of abuse from brain tissue.
Tags: Amphetamine, Benzoylecgonine, Brain, Cocaine, Codeine, Column, Diazepam, Doxepin, Drugs of Abuse, English, Forensic, GC-MS, Ibuprofen, Methadone, Metoprolol, Morphine, Phenobarbitone, Posters

Matrix components, particularly salts, proteins and phospholipids, can lead to ion suppression resulting in inaccurate quantitation and reduced detection limits. Resin-based mixed-mode cation exchange SPE sorbents are widely used for the extraction of basic compounds from biological fluids. The dual retention mechanism allows a two stage interference wash protocol, which results in extremely clean extracts. This poster will investigate the performance of EVOLUTE™ CX for the extraction of a wide range of basic drugs from plasma, showing high analyte recovery and advanced extract cleanliness. The analyte suite was selected to cover a variety of basic analytes with wide ranging polarities (LogP values). Extract cleanliness experiments were performed showing overall ion suppression, then individual matrix components examined in terms of protein and phospholipid removal.
Tags: Plasma, Posters

Matrix components, particularly salts, proteins and phospholipids, can lead to ion suppression resulting in inaccurate quantitation and reduced detection limits. Resin-based mixed-mode cation exchange SPE sorbents are widely used for the extraction of basic compounds from biological fluids. The dual retention mechanism allows a two stage interference wash protocol, which results in extremely clean extracts. This poster will investigate the performance of EVOLUTE™ CX for the extraction of a wide range of basic drugs from plasma, showing high analyte recovery and advanced extract cleanliness. The analyte suite was selected to cover a variety of basic analytes with wide ranging polarities (LogP values). Extract cleanliness experiments were performed showing overall ion suppression, then individual matrix components examined in terms of protein and phospholipid removal.
Tags: Basic drugs, Biological fluids, Posters

Sample preparation is an essential technique prior to LC-MS/MS analysis of drugs in biological fluid samples to remove unwanted matrix components. These matrix components, particularly salts, proteins and phospholipids, can lead to ion suppression resulting in inaccurate quantitation and reduced detection limits. Resin-based mixed-mode cation exchange SPE sorbents are widely used for the extraction of basic compounds from biological fluids. The dual retention mechanism allows a two stage interference wash protocol, which results in extremely clean extracts. This poster investigates the performance of EVOLUTE™ CX for overall ion suppression, then individual matrix components examined in terms of protein and phospholipid removal.
Tags: Posters