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Biotage® SNAP Bio
Wide Pore Media for Better Peptide Separation
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Highlighted Documents
More Biotage® SNAP Bio documents »
Biotage® SNAP Bio flash cartridges were developed with a small particle size (20 μm) and large pore size (300 Å) to provide increased resolution and the effective separation of complex peptide mixtures.
| Sizes | 10 g, 25 g, 50 g, 100 g |
| Tube Material | Inert medical-grade polypropylene |
| Frit Material | Inert polyethylene |
| Pressure Range | 0-100 psi / 7 bar |
| Media | Biotage® HP-BioSphere C18 - 20 micron spherical silica |
| Media | Biotage® HP-BioSphere C4 - 20 micron spherical silica |
| Resolution | >6000 M/n (plates per metre); >4000 for 10 g |
| Compatibility | Universal Luer connections |
» APPLICATION NOTES
Peptide Purification with Biotage® SNAP Bio Cartridges: Achieving High Purity and High Recovery
This work demonstrates that peptide purification efficiency by flash
chromatography is improved with Biotage® SNAP Bio cartridges containing a
spherical wide pore stationary under a wide variety of purification conditions.
Tags: Application Notes
Flash chromatography, a fast and efficient technique for purification of peptides Part 2
Improved separation Performance with Biotage® SNAP Bio
wide pore Media cartridges
Tags: Application Notes
Flash chromatography, a fast and efficient technique for purification of peptides Part 1
Improved Separation Performance Using Biotage®SNAP Bio
Wide Pore media Cartridges – Part 1
Tags: Application Notes
Mass Directed Flash Purification of Amphipathic Peptide '18A' Using Isolera™ Dalton
In this study we synthesized two closely related amphipathic peptides and show the mass directed flash purification of non-acetylated ‘18A’ from a crude mixture containing a single residue deletion sequence ‘17A’ (-Glu residue) using the Biotage® Isolera Dalton™.
Tags: Application Notes
Separation of Peptidic Deletion Sequences with Reversed Phase Mass Directed Flash Purification Using Isolera™ Dalton and SNAP KP-C18-HS Flash Cartridges
This application note shows how the two
closely related peptide structures (MW 2139 and 2267) were
separated using reversed phase mass directed flash chromatography
with Isolera™ Dalton.
Tags: Application Notes
» BROCHURES
Peptide Synthesis Workflow - Synthesis, Purification, and Evaporation Solutions
Biotage has developed a range
of tools to improve the peptide synthesis workflow,
from synthesis of crude peptides through to the final
purified product. These solutions make your workflow
more efficient and improve the quality of peptides
you synthesize. Our workflow solutions are used in
pharma, biotech, CRO and academic laboratories
throughout the world.
Tags: Brochures
» MANUALS & USER GUIDES
White Paper - A Holistic Approach to the Peptide Workflow
Producing peptides for research is challenging. Biotage has developed
a holistic approach to the entire peptide workflow via an automated solution, designed for dedicated peptide researchers and those new to the field.
Tags: Manuals & User Guides,
Product Notes,
White Papers
» POSTERS
High Performance Flash Chromatography. An Alternative Technique to Rapidly and Efficiently Purify Crude Synthetic Peptides.
We investigate the use of reversed-phase high performance flash chromatography (HPFC) to quickly purify large quantities of crude synthetic peptides.
(6th Solid Phase Peptide Synthesis Symposium & 12th Australian Peptide Conference, Queensland, Australia, Oct. 2017)
Tags: Posters
Alternative Strategies for Purification of Fully Protected Peptides Using Flash Chromatography
Improvements in solid phase peptide synthesis strategies and
development of resin linkages susceptible to low acid cleavage
conditions has enabled synthesis of long peptides while keeping the
protecting groups intact. This strategy is now used for the
preparation of chemically synthesized proteins, wherein shorter
peptide fragments are ligated together. They are also found in the
synthesis of peptide macrocycles that utilize head-to-tail cyclization strategies. Although linear synthesis of protected peptides is generally straightforward, purification of these compounds using traditional reversed phase methods is quite challenging. Herein we describe the use of normal phase chromatography for purification of fully protected peptides.
Tags: Posters
Improving Peptide Purification via Flash Column Chromatography by Modulating Mobile Phase pH
Although capable of very high resolution, RP-HPLC is often limited by low column loading capacity, therefore demanding a significant time investment for peptide purification. As an alternative strategy, reversed-phase flash chromatography can also be used to purify synthetic peptides. The larger particle size used in flash column chromatography enables much larger loading capacity, thereby significantly reducing the time required for peptide purification.
Tags: Posters
» PRODUCT NOTES
White Paper - A Holistic Approach to the Peptide Workflow
Producing peptides for research is challenging. Biotage has developed
a holistic approach to the entire peptide workflow via an automated solution, designed for dedicated peptide researchers and those new to the field.
Tags: Manuals & User Guides,
Product Notes,
White Papers
Biotage® SNAP Bio C18 & C4 300 Å Cartridges
Biotage® SNAP Bio flash cartridges were developed with a small
particle size (20 μm) and large pore size (300 Å) to provide
increased resolution and effective separation of complex
peptide mixtures. Depending on the quality of the peptides
synthesized, flash chromatography can be used either as the
sole method of purification or as a front end clean-up prior to
RP-HPLC.
SNAP Bio offers close to prep-HPLC performance in a flash
format at a fraction of the cost
Tags: Product Notes
» WHITE PAPERS
Achieve Highly Pure Peptides with High Performance Flash Chromatography
Peptide purification via flash
chromatography has recently been demonstrated as a viable
alternative to the more standard HPLC methods currently
utilized. Flash chromatography offers peptide chemists the
advantage of significantly greater loading capacity reducing the
overall purification time but with a compromise of decreased
peak resolution. Herein we present several strategies that,
when implemented, allow for very high purity peptide samples
purified by flash chromatography.
Tags: White Papers
White Paper - A Holistic Approach to the Peptide Workflow
Producing peptides for research is challenging. Biotage has developed
a holistic approach to the entire peptide workflow via an automated solution, designed for dedicated peptide researchers and those new to the field.
Tags: Manuals & User Guides,
Product Notes,
White Papers
Search all documents »
WEBINARS
Achieve Highly Pure Peptides Using High Performance Flash Chromatography
News
11 January 2019How St. John's University Accelerated their Cancer Research
"How can I take a two hour purification using old-school chromatography, and shorten it?" was the question Dr. Aaron Muth at St. John's University in New York asked himself. "By using the Biotage I...
20 July 2018Achieve Highly Pure Peptides Using High Performance Flash Chromatography
Learn how to overcome loss of resolution in peptide purification in this webinar recording from Biotage, recorded on July 12 2018. Standard HPLC-mediated purification is often the mo...
13 November 2017A Holistic Approach to the Peptide Workflow in Drug Discovery
The study of peptides, especially for therapeutic use, has grown significantly in recent years. However, producing peptides for such research is challenging, not just in the s...
02 November 2017Peptide Purification Using High Performance Flash Chromatography
The purification step is one of the main bottlenecks in the peptide synthesis workflow. Preparative reversed-phase HPLC is normally the method of choice for purification of synthetic peptides, but ...
13 July 2017New Peptide Synthesis Workflow Brochure
Biotage is pleased to announce the release of the new Peptide Synthesis Workflow – Synthesis, Purification, and Evaporation Solutions Brochure The typical peptide synthesis workflow broadly ...
06 February 2017"That is why we bought the second Alstra"
Dr. Andrew Jamieson leads a team of peptide chemists at the University of Glasgow, where they synthesize complex molecules for chemical biology applications. An important tool that contributes to t...
19 January 2017Flash Purification of Peptides - Part 2
Biotage® SNAP Bio flash cartridges were developed with a small particle size (20 μm) and large pore size (~300 Å) to provide increased resolution and effective separation of complex pe...
10 January 2017Flash Purification of Peptides - Is it Possible?
Recent advances in flash purification using 20–25 micron spherical particles makes flash chromatography an efficient technique for synthetic peptide purification. This application note from B...
13 December 2016Webinar on Demand: Peptide Purification using Flash Chromatography
This webinar discusses some recent work on the use of flash chromatography for the purification of synthetic peptides as a complementary alternative to standard RP-HPLC methods. Included herein are...
07 December 2016Press Release: New Flash Cartridges for Peptide Purification from Biotage
Uppsala, Sweden, 7th December, 2016 – Biotage (STO: BIOT), a leading global supplier of solutions and technology for analytical, medicinal and peptide chemistry, is pleased to announce the la...
17 September 2014Peptide Association Links
Relevant peptide associations. Please contact webmaster to add to, or update this page. Asia Pacific Protein Association Association of Biomolecular Resource Facilities American Peptide Societ...
01 May 2012Celebrating a Decade of Excellence in Microwave Peptide Synthesis
For the past decade our customers have been using Biotage's microwave synthesis systems for solid phase synthesis of peptides, peptoids and peptidomimetics. In celebration of this decade of excelle...
Biotage® SNAP KP-C18-HS
Reversed-phase flash chromatography is a very effective purification technique. Its main application areas include polar, ionizable and highly lipophilic compounds which cannot easily be separated by normal-phase techniques.
Isolera™ Dalton
Isolera™ Dalton is the most advanced and innovative flash purification system available, designed by people who understand flash chromatography for the chemists who need it.
"Pure convenience! Just pure convenience! It lets us perform purification and analysis at the same time. It gives us tremendous power to isolate unknown compounds.”
- Hiroki Nishii, Chugai Pharmaceutical’s Kamakura Research Laboratories
Biotage® SNAP Ultra C18
The latest, highest performing reversed phase C18 spherical silica flash cartridges available.
Isolera™ Spektra Systems with ACI™ and Assist
Isolera™ Spektra is a high performance ACI™ Accelerated Chromatographic Isolation system. Available as Isolera Spektra One, Four and LS, with time, efficiency and cost saving purification run features that are normally only found on the world’s most expensive high-end chromatography systems.
With Isolera™ Assist you get the benefits of the feature-rich Isolera Flash Purification Systems in a simple and easy to use interface. It ensures you achieve the best results, regardless of your experience level.
The purification step (RP-HPLC) is one of the main bottlenecks in the peptide synthesis workflow, limited by small loading amounts, long separation times, poor recoveries and high costs. Biotage® SNAP Bio cartridges offer similar performance, in a flash format, at a fraction of the cost. They have an increased loading capability compared to prep RP-HPLC, enabling more peptide to be processed in a single injection and faster, more efficient peptide purification.
Biotage® SNAP Bio flash cartridges are a powerful tool to address the purification needs of peptide chemists.
CONSUMABLES
| Part No. | Description | Product type | Pack Size | Price | ||
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FBIO-0411-0010 | Biotage® SNAP Bio C18 300 Å 10 g, 2/cs | Consumables | 2 | Log in for price | |
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FBIO-0411-0025 | Biotage® SNAP Bio C18 300 Å 25 g, 2/cs | Consumables | 2 | Log in for price | |
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FBIO-0411-0050 | Biotage® SNAP Bio C18 300 Å 50 g, 2/cs | Consumables | 2 | Log in for price | |
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FBIO-0411-0100 | Biotage® SNAP Bio C18 300 Å 100 g, 2/cs | Consumables | 2 | Log in for price | |
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FBIO-0412-0010 | Biotage® SNAP Bio C4 300 Å 10 g, 2/cs | Consumables | 2 | Log in for price | |
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FBIO-0412-0025 | Biotage® SNAP Bio C4 300 Å 25 g, 2/cs | Consumables | 2 | Log in for price | |
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FBIO-0412-0050 | Biotage® SNAP Bio C4 300 Å 50 g, 2/cs | Consumables | 2 | Log in for price | |
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FBIO-0412-0100 | Biotage® SNAP Bio C4 300 Å 100 g, 2/cs | Consumables | 2 | Log in for price | |