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- Automated Sample Preparation Instruments
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- ISOLUTE® FILTER+ High Performance Filtration Plates
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- Biotage® Sfär - Overview
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Biotage® SNAP Bio
Wide Pore Media for Better Peptide Separation
This product was withdrawn on October 1st, 2019.
Please check out here the corresponding column in the Biotage® Sfär family:
C18 media: Biotage® Sfär Bio C18 Duo 300 Å 20 μm or
C4 media: Biotage® Sfär Bio C4 Duo 300 Å 20 μm
The purification step (RP-HPLC) is one of the main bottlenecks in the peptide synthesis workflow, limited by small loading amounts, long separation times, poor recoveries and high costs. Biotage® Sfär Bio cartridges offer similar performance, in a flash format, at a fraction of the cost. They have an increased loading capability compared to prep RP-HPLC, enabling more peptide to be processed in a single injection and faster, more efficient peptide purification.
Biotage® Sfär Bio flash cartridges are a powerful tool to address the purification needs of peptide chemists.
| Sizes | 10 g, 25 g, 50 g, 100 g |
| Tube Material | Inert medical-grade polypropylene |
| Frit Material | Inert polyethylene |
| Pressure Range | 0-100 psi / 7 bar |
| Media | Biotage® HP-BioSphere C18 - 20 micron spherical silica |
| Media | Biotage® HP-BioSphere C4 - 20 micron spherical silica |
| Resolution | >6000 M/n (plates per metre); >4000 for 10 g |
| Compatibility | Universal Luer connections |
» APPLICATION NOTES
Peptide Purification with Biotage® SNAP Bio Cartridges: Achieving High Purity and High Recovery
This work demonstrates that peptide purification efficiency by flash
chromatography is improved with Biotage® SNAP Bio cartridges containing a
spherical wide pore stationary under a wide variety of purification conditions.
Tags: Application Notes
Flash chromatography, a fast and efficient technique for purification of peptides Part 2
Improved separation Performance with Biotage® SNAP Bio
wide pore Media cartridges
Tags: Application Notes
Flash chromatography, a fast and efficient technique for purification of peptides Part 1
Improved Separation Performance Using Biotage®SNAP Bio
Wide Pore media Cartridges – Part 1
Tags: Application Notes
Mass Directed Flash Purification of Amphipathic Peptide '18A' Using Isolera™ Dalton
In this study we synthesized two closely related amphipathic peptides and show the mass directed flash purification of non-acetylated ‘18A’ from a crude mixture containing a single residue deletion sequence ‘17A’ (-Glu residue) using the Biotage® Isolera Dalton™.
Tags: Application Notes
Separation of Peptidic Deletion Sequences with Reversed Phase Mass Directed Flash Purification Using Isolera™ Dalton and SNAP KP-C18-HS Flash Cartridges
This application note shows how the two
closely related peptide structures (MW 2139 and 2267) were
separated using reversed phase mass directed flash chromatography
with Isolera™ Dalton.
Tags: Application Notes
» MANUALS & USER GUIDES
Discontinuation Letter Biotage® SNAP and SNAP Ultra Columns
This letter is to inform customers that effective October 1st,
2019 Biotage will phase out the SNAP family of columns.
Tags: English,
Manuals & User Guides
White Paper - A Holistic Approach to the Peptide Workflow
Producing peptides for research is challenging. Biotage has developed
a holistic approach to the entire peptide workflow via an automated solution, designed for dedicated peptide researchers and those new to the field.
Tags: Manuals & User Guides,
Product Notes,
White Papers
» POSTERS
High Performance Flash Chromatography. An Alternative Technique to Rapidly and Efficiently Purify Crude Synthetic Peptides.
We investigate the use of reversed-phase high performance flash chromatography (HPFC) to quickly purify large quantities of crude synthetic peptides.
(6th Solid Phase Peptide Synthesis Symposium & 12th Australian Peptide Conference, Queensland, Australia, Oct. 2017)
Tags: Posters
Alternative Strategies for Purification of Fully Protected Peptides Using Flash Chromatography
Improvements in solid phase peptide synthesis strategies and
development of resin linkages susceptible to low acid cleavage
conditions has enabled synthesis of long peptides while keeping the
protecting groups intact. This strategy is now used for the
preparation of chemically synthesized proteins, wherein shorter
peptide fragments are ligated together. They are also found in the
synthesis of peptide macrocycles that utilize head-to-tail cyclization strategies. Although linear synthesis of protected peptides is generally straightforward, purification of these compounds using traditional reversed phase methods is quite challenging. Herein we describe the use of normal phase chromatography for purification of fully protected peptides.
Tags: Posters
Improving Peptide Purification via Flash Column Chromatography by Modulating Mobile Phase pH
Although capable of very high resolution, RP-HPLC is often limited by low column loading capacity, therefore demanding a significant time investment for peptide purification. As an alternative strategy, reversed-phase flash chromatography can also be used to purify synthetic peptides. The larger particle size used in flash column chromatography enables much larger loading capacity, thereby significantly reducing the time required for peptide purification.
Tags: Posters
» PRODUCT NOTES
White Paper - A Holistic Approach to the Peptide Workflow
Producing peptides for research is challenging. Biotage has developed
a holistic approach to the entire peptide workflow via an automated solution, designed for dedicated peptide researchers and those new to the field.
Tags: Manuals & User Guides,
Product Notes,
White Papers
Biotage® SNAP Bio C18 & C4 300 Å Cartridges
Biotage® SNAP Bio flash cartridges were developed with a small
particle size (20 μm) and large pore size (300 Å) to provide
increased resolution and effective separation of complex
peptide mixtures. Depending on the quality of the peptides
synthesized, flash chromatography can be used either as the
sole method of purification or as a front end clean-up prior to
RP-HPLC.
SNAP Bio offers close to prep-HPLC performance in a flash
format at a fraction of the cost
Tags: Product Notes
» WHITE PAPERS
Achieve Highly Pure Peptides with High Performance Flash Chromatography
Peptide purification via flash
chromatography has recently been demonstrated as a viable
alternative to the more standard HPLC methods currently
utilized. Flash chromatography offers peptide chemists the
advantage of significantly greater loading capacity reducing the
overall purification time but with a compromise of decreased
peak resolution. Herein we present several strategies that,
when implemented, allow for very high purity peptide samples
purified by flash chromatography.
Tags: White Papers
White Paper - A Holistic Approach to the Peptide Workflow
Producing peptides for research is challenging. Biotage has developed
a holistic approach to the entire peptide workflow via an automated solution, designed for dedicated peptide researchers and those new to the field.
Tags: Manuals & User Guides,
Product Notes,
White Papers
Search all documents »
WEBINARS
Achieve Highly Pure Peptides Using High Performance Flash Chromatography
News
28 June 2019Notice of Discontinuation of Biotage® SNAP Flash Columns
Dear Valued Customers, We value your continued support and therefore want to notify you of a change in our product line - the discontinuation of the Biotage® SNAP and Biotage® SNAP Ultra p...
11 January 2019How St. John's University Accelerated their Cancer Research
"How can I take a two hour purification using old-school chromatography, and shorten it?" was the question Dr. Aaron Muth at St. John's University in New York asked himself. "By using the Biotage I...
20 July 2018Achieve Highly Pure Peptides Using High Performance Flash Chromatography
Learn how to overcome loss of resolution in peptide purification in this webinar recording from Biotage, recorded on July 12 2018. Standard HPLC-mediated purification is often the mo...
13 November 2017A Holistic Approach to the Peptide Workflow in Drug Discovery
The study of peptides, especially for therapeutic use, has grown significantly in recent years. However, producing peptides for such research is challenging, not just in the s...
02 November 2017Peptide Purification Using High Performance Flash Chromatography
The purification step is one of the main bottlenecks in the peptide synthesis workflow. Preparative reversed-phase HPLC is normally the method of choice for purification of synthetic peptides, but ...
13 July 2017New Peptide Synthesis Workflow Brochure
Biotage is pleased to announce the release of the new Peptide Synthesis Workflow – Synthesis, Purification, and Evaporation Solutions Brochure The typical peptide synthesis workflow broadly ...
06 February 2017"That is why we bought the second Alstra"
Dr. Andrew Jamieson leads a team of peptide chemists at the University of Glasgow, where they synthesize complex molecules for chemical biology applications. An important tool that contributes to t...
19 January 2017Flash Purification of Peptides - Part 2
Biotage® SNAP Bio flash cartridges were developed with a small particle size (20 μm) and large pore size (~300 Å) to provide increased resolution and effective separation of complex pe...
10 January 2017Flash Purification of Peptides - Is it Possible?
Recent advances in flash purification using 20–25 micron spherical particles makes flash chromatography an efficient technique for synthetic peptide purification. This application note from B...
13 December 2016Webinar on Demand: Peptide Purification using Flash Chromatography
This webinar discusses some recent work on the use of flash chromatography for the purification of synthetic peptides as a complementary alternative to standard RP-HPLC methods. Included herein are...
07 December 2016Press Release: New Flash Cartridges for Peptide Purification from Biotage
Uppsala, Sweden, 7th December, 2016 – Biotage (STO: BIOT), a leading global supplier of solutions and technology for analytical, medicinal and peptide chemistry, is pleased to announce the la...
Biotage® Sfär Bio C18 Duo 300 Å 20 μm
High performance flash chromatography is a very efficient technique to dramatically clean-up synthetic peptides. Biotage® Sfär Bio flash columns were developed with a small particle size (20 μm) and large pore size (300 Å) to provide increased resolution and the effective separation of complex peptide mixtures.
Biotage® Sfär Bio C4 D Duo 300 Å 20 μm
High performance flash chromatography is a very efficient technique to dramatically clean-up synthetic peptides. Biotage® Sfär Bio flash columns were developed with a small particle size (20 μm) and large pore size (300 Å) to provide increased resolution and the effective separation of complex peptide mixtures.
