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POROS CaptureSelect™ IgM PhyTip columns for purification of IgMs
POROS CaptureSelect™ IgM affinity matrix is used for purification of IgM antibodies by binding to the Fc region of IgM antibodies. This affinity media consist of a CaptureSelect affinity ligand that is coupled to NHS-activated Sepharose 4 Fast Flow® resin (Cytiva). By binding to an unique domain on the Fc part of IgM, this affinity media enables highly specific purification of human and mouse IgMs without cross reactivity with human and mouse IgG and IgA from complex sources like plasma, serum and cell culture supernatants.
The affinity ligands of CaptureSelect™ medias from ThermoFisher are based on a recombinant antibody fragment of only 14 kDa in size that can be adapted for highly selective binding to certain parts or antibodies and antibody fragments.
PhyNexus, now part of Biotage, developed the PhyTip® column in 2002 and have supported researchers with automated protein purification ever since. PhyTip columns purify samples by repeated back-and-forth cycles - a process called dual flow chromatography which allows complete binding interactions in each purification step.
Due to maximization of binding of the sample, less protein is lost during the purification process. The result is very high sample recoveries.
Our unique column design with a packed resin bed results in binding of the entire sample to a small resin bed volume. The purified sample can be eluted in small volumes, resulting in a highly concentrated sample.
Effective purification by PhyTip columns enables a full purification protocol of 96 samples in as little as 15 minutes.
Our PhyTip columns are available for all major liquid handling platforms to enable you to use your existing system that you may already have in your lab for automated protein purification. If you don't have a system, we offer automation solutions for protein purification by PhyTip columns.
