Increase efficiency with automated solid-phase peptide synthesis

Automating solid-phase peptide synthesis, the first step in the peptide workflow, offers key benefits:

Rapidly generate libraries with high-throughput synthesis
Increase productivity by optimizing reaction times and ensuring consistent peptide quality
Minimize human error, side reactions, and incomplete synthesis
Synthesize a wide range of peptides, from simple to complex sequences
Optimize reagent use, reducing waste and making the process more cost-effective and environmentally friendly

PNG-biotage-initiator-alstra-peptide-synthesis-vial-loading-tool

5 tips for successful solid phase peptide synthesis

Peptide synthesis involves a few straightforward organic reactions, yet crude yield and purity can vary significantly. To help you achieve consistent success, we've compiled essential tips and tricks for optimizing solid-phase peptide synthesis.

PNG-biotage-initiator-alstra-peptide-synthesis-30ml-reactor-vial-closeup-356290

How to choose the right resin for peptide synthesis

The resin you select plays a crucial role in the success of your peptide synthesis. Read the first part of this blog series to discover resin recommendations for mid- to long-length peptides.

biotage-university-of-vienna-peptide-synthesis

Rapid production of structurally diverse peptides

The University of Vienna’s Institute of Inorganic Chemistry synthesizes a wide range of peptidic radiotracers for clinical applications. Their research also demonstrates the integration of triazole-based compounds into peptide backbones using a microwave synthesizer.

PNG-biotage-initiator-alstra-peptide-synthesis-system-needle-closeup-356017

Enhancing solid-phase peptide synthesis with green chemistry principles

This webinar presents strategies to reduce solvents and reagents in solid-phase peptide synthesis, as well as how to eliminate dimethylformamide (DMF), and leverage automation platforms to maintain or improve crude purity.

Peptide synthesizers tailored to your needs

Biotage provides peptide synthesizers for both small-scale and high-throughput synthesis in drug discovery and research labs. Explore our solutions to find the right fit for your needs.

Related products

Biotage® Initiator+ Alstra™

The Biotage® Initiator+ Alstra™ is a fully automated, single-channel microwave peptide synthesizer with an intuitive touchscreen interface, enabling efficient sequential synthesis of single peptides.

Syro I

The Syro I is a fully automated, multi-channel peptide synthesizer with a one-arm pipetting robot. Featuring one reactor block and optional additional blocks, it’s ideal for lab-scale, parallel synthesis requirements.

Syro II

The Syro II is a fully automated, multi-channel peptide synthesizer with a two-arm pipetting robot for fast reagent handling. With two reactor blocks and optional additional blocks, it provides the highest throughput for lab-scale parallel synthesis requirements.

Biotage insights: Small molecules & synthetic therapeutics

Optimizing peptide purification with flash chromatography

A study shows flash chromatography efficiently purifies peptides, allowing larger loads per injection while saving time, solvent, and costs versus HPLC.

Does media choice matter for peptide library purification using solid phase extraction?

SPE is emerging as a strategy for peptide library clean up in parallel.

What is the difference between solid vs. liquid particles in ELSD signal?

ELSD is useful for finding molecules that have no chromophore during flash purification. Here we show how signal intensity can vary between compounds!

More blogs

Resources

Documents

Other steps in the workflow

Purification is often the main bottleneck in the peptide workflow. High-performance flash chromatography (HPFC) coupled with C18 media is optimized for peptide purification, enhancing peptide workflow efficiency further.

Flash purification of peptides

Projects requiring high-throughput synthesis often encounter significant purification bottlenecks. Automated parallel purification addresses this issue by streamlining the process, effectively bridging the efficiency gap between peptide library synthesis and purification.

Parallel purification of peptides

Peptide purification often generates a lot of fractions that require time-consuming concentration and pooling. Automated evaporation technologies can quickly and gently remove both aqueous and organic solvents, thus streamlining the process.

Evaporation for peptides