Fast and gentle evaporation for peptide workflows

Peptide purification generates a lot of fractions that require time-consuming concentration and pooling. Our automated evaporation solutions remove aqueous and organic solvents quickly and gently, preventing bumping and streamlining your workflow.

Reduce or remove cleavage cocktails before ether precipitation
Concentrate and pool flash or HPLC fractions before freeze-drying
Efficiently remove high-boiling solvents like DMF and DMSO
Integrate evaporation flexibly, either post-synthesis or post-purification

Alternative strategy for handling cleaved peptides from resin

After peptide synthesis, ether precipitation and lyophilization are common steps before purification. But is there a more efficient approach? In this post, we explore an alternative strategy for processing cleaved peptides from resin. This method saves time, reduces hazardous reagents, and preserves your peptide’s integrity.

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Typical solvent evaporation times

Biotage® V-10 Touch evaporation system features optimized built-in methods that prevent overheating and bumping while maximizing evaporation speed. Even challenging solvents like DMSO can evaporate at a rate of 0.5 mL per minute or more.

Removing peptide cleavage cocktail

From peptide synthesis to cleavage and dried peptide, explore the process in just 3 minutes using the Biotage suite of tools.

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Peptide synthesis workflow: synthesis, purification, and evaporation

Peptide synthesis workflows include synthesis, purification, and evaporation. While synthesis is key, purification and evaporation often cause bottlenecks. Biotage provides tools to streamline the entire process, from crude synthesis to final purification, supporting pharma, biotech, CROs, and academia worldwide.

Biotage insights: Small molecules & synthetic therapeutics

Optimizing peptide purification with flash chromatography

A study shows flash chromatography efficiently purifies peptides, allowing larger loads per injection while saving time, solvent, and costs versus HPLC.

Does media choice matter for peptide library purification using solid phase extraction?

SPE is emerging as a strategy for peptide library clean up in parallel.

What is the difference between solid vs. liquid particles in ELSD signal?

ELSD is useful for finding molecules that have no chromophore during flash purification. Here we show how signal intensity can vary between compounds!

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Biotage® V-10 Touch

The Biotage® V-10 Touch quickly dries samples dissolved in aqueous and organic solvents. It efficiently evaporates HPLC fractions from purification, high-boiling-point solvents from synthesis, and enables dry-down onto silica for easier dry loading before purification.

Biotage® Selekt

A two column channel system with a colorful and intuitive user interface on a large touchscreen enables even the most complex tasks to become simplicity itself. High flowrate and pressure make separation faster and greener due to less solvent consumption.

Biotage® Sfär Bio C18 D

High-performance flash chromatography is a powerful technique for purifying synthetic peptides. Biotage® Sfär Bio flash columns, featuring a small 20 μm particle size and large 300 Å pores, deliver enhanced resolution and efficient separation of complex peptide mixtures.

Other steps in the workflow

Biotage offer a range of manual, semi-automated and fully automated systems depending on your peptide synthesis needs. Whether you require a system that makes a single peptide or multiple peptides in parallel, we have an instrument to suit your requirements.

Peptide synthesis

Purification is often the main bottleneck in the peptide workflow. High-performance flash chromatography (HPFC) coupled with C18 media is optimized for peptide purification, enhancing peptide workflow efficiency further.

Flash purification of peptides

Projects requiring high-throughput synthesis often encounter significant purification bottlenecks. Automated parallel purification addresses this issue by streamlining the process, effectively bridging the efficiency gap between peptide library synthesis and purification.

Parallel purification of peptides