Evaporation of Peptides

Evaporation of cleavage cocktails and preparative HPLC fractions are peptide synthesis process steps that can take hours or days and, particularly for larger HPLC fractions, can require constant attention to avoid bumping in traditional evaporators. Biotage has evaporation solutions that reduce the time to less than 15 minutes and eliminate bumping with our fully automated and walk-away reliable V-10 Touch Evaporator.

Evaporation of Peptides

Evaporation is an important step in the peptide workflow. Now many of the challenges of dealing with insoluble peptides are a thing of the past - the V-10 Touch is so capable that even difficult to remove solvents like DMSO can be evaporated at the rate of 0.5 mL per minute or more.

Where can V-10 Touch help peptide chemists?

Removal of cleavage cocktails, either completely or reducing the volume before ether precipitation.
Concentration and pooling of flash or HPLC fractions prior to freeze drying.
Removal of high boiling solvents such as DMF, DMSO that are used in synthesis reactions or as storage solvents.
Can be used post synthesis or post purification.

Related products

Biotage® V-10 Touch

The Biotage® V-10 Touch quickly dries samples dissolved in aqueous and organic solvents. It efficiently evaporates HPLC fractions from purification, high-boiling-point solvents from synthesis, and enables dry-down onto silica for easier dry loading before purification.

Biotage® Selekt

A two column channel system with a colorful and intuitive user interface on a large touchscreen enables even the most complex tasks to become simplicity itself. High flowrate and pressure make separation faster and greener due to less solvent consumption.

Biotage® Sfär Bio C18 D

High-performance flash chromatography is a powerful technique for purifying synthetic peptides. Biotage® Sfär Bio flash columns, featuring a small 20 μm particle size and large 300 Å pores, deliver enhanced resolution and efficient separation of complex peptide mixtures.

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Since the development of Fmoc-based solid phase peptide synthesis, a wide variety of cleavage cocktails have emerged. Each cleavage cocktail contains a unique combination of scavengers designed to prevent either side reactions mediated by the ...

Peptide Synthesis Workflow - Synthesis, Purification, and Evaporation Solutions

The typical peptide synthesis workflow broadly involves synthesis, purification and evaporation steps to give the final pure peptide. Although the synthesis step is the most important part of the workflow, purification and evaporation issues can impact dramatically on the efficiency of your workflow and are the cause of many bottlenecks. Biotage has developed a range of tools to improve the peptide synthesis workflow, from synthesis of crude peptides through to the final purified product. Our workflow solutions are used in pharma, biotech, CRO and academic laboratories throughout the world.

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Other steps in the workflow

Biotage offer a range of manual, semi-automated and fully automated systems depending on your peptide synthesis needs. Whether you require a system that makes a single peptide or multiple peptides in parallel, we have an instrument to suit your requirements.

Peptide Synthesis

Purification is often the main bottleneck in the peptide workflow. High-performance flash chromatography (HPFC) coupled with C18 media is optimized for peptide purification, enhancing peptide workflow efficiency further.

Flash purification of peptides

Projects requiring high-throughput synthesis often encounter significant purification bottlenecks. Automated parallel purification addresses this issue by streamlining the process, effectively bridging the efficiency gap between peptide library synthesis and purification.

Parallel purification of peptides

Evaporation of Peptides