Automate high-throughput parallel peptide purification

High-throughput peptide synthesis is essential for early-stage drug discovery, but purification often creates bottlenecks. Traditional RP-HPLC methods are slow and solvent-intensive. Automated, plate-based parallel purification offers a faster, more sustainable alternative:

Achieve high peptide purity with simple, pre-optimized protocols
Purify up to 96 peptides in parallel for increased throughout
Cut solvent use for a more environmentally friendly workflow
Lower costs by improving efficiency and productivity

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Two solutions, one goal

Biotage offers two automated, plate-based solutions to streamline peptide purification after synthesis.

Biotage® PeptiRen-96: A versatile solution for all peptide types, using reversed-phase C18 media for solid-phase extraction (SPE) to achieve over 60% average purity, making it ideal for preliminary screening.
Biotage® PeptiPEC-96: Optimized for synthetic peptides with a free N-terminus, delivering over 80% average purity and improving validation assays and structure-activity relationship (SAR) studies.

Novel methodology for pure peptides

Biotage® PeptiPEC-96 parallel peptide purification employs a catch-and-release methodology based on the PurePep® EasyClean (PEC™) technology from Gyros Protein Technologies. Watch the video to discover how it works.

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Parallel peptide workflows for streamlined library delivery

This white paper outlines a parallel peptide workflow using Syro parallel peptide synthesizers and Biotage® PeptiRen-96 plates with optimized solid-phase extraction protocols, enhancing library purity and eliminating labor-intensive ether precipitation. Together, these tools enable an efficient peptide library discovery program.

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Optimizing peptide library purification: data-driven insights

Interest in large synthetic peptide libraries is growing, requiring workflow changes to prevent bottlenecks. This webinar presents data on parallel peptide purification, replacing manual steps while preserving integrity, yield, and potentially improving purity.

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Parallel purification of 192 peptides in plate-based format

This application note demonstrates the use of Biotage® PeptiPEC-96 High-Throughput Kits and the Biotage® Extrahera™-Peptide workstation for the automated purification of a peptide library containing 192 SARS-CoV-2 epitopes, designed for use in T-cell response assays.

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Solid-phase extraction media comparison for synthetic peptide clean-up

Automating solid-phase extraction (SPE) in a 96-well plate format allows high-throughput peptide synthesis and parallel sample processing. However, selecting the right media that interacts predictably with peptides is key to achieving optimal results. This application note compares the performance of Biotage® PeptiRen C18 media with other commercially available options.

Biotage insights: Small molecules & synthetic therapeutics

Optimizing peptide purification with flash chromatography

A study shows flash chromatography efficiently purifies peptides, allowing larger loads per injection while saving time, solvent, and costs versus HPLC.

Does media choice matter for peptide library purification using solid phase extraction?

SPE is emerging as a strategy for peptide library clean up in parallel.

What is the difference between solid vs. liquid particles in ELSD signal?

ELSD is useful for finding molecules that have no chromophore during flash purification. Here we show how signal intensity can vary between compounds!

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Related products

Biotage® Extrahera™ Classic

A powerful, user-friendly workstation for automated, high-throughput purification of peptides with Biotage® PeptiRen-96 Plates and Biotage® PeptiPEC-96 High-Throughput Kit.

Biotage® PeptiRen-96 & Biotage® PeptiPEC-96

Automated, high-throughput peptide purification solutions for the Biotage® Extrahera Classic workstation, optimizing efficiency while minimizing solvent use. Designed to streamline peptide screening and discovery for better results.

Other steps in the workflow

Biotage offer a range of manual, semi-automated and fully automated systems depending on your peptide synthesis needs. Whether you require a system that makes a single peptide or multiple peptides in parallel, we have an instrument to suit your requirements.

Peptide synthesis

Peptide purification often generates a lot of fractions that require time-consuming concentration and pooling. Automated evaporation technologies can quickly and gently remove both aqueous and organic solvents, thus streamlining the process.

Evaporation of peptides