Evaporation

Removing the solvent from your sample

Once a reaction is complete and the target of the synthesis has been purified to the required level, the solvent in which the sample is dissolved needs to be removed. In many laboratories, this can be the most time consuming and laborious part of a synthetic procedure. Rotary evaporators are ubiquitous in chemistry laboratories, and most chemists have spent many hours carefully tending evaporations to remove solvents, watchful for bumping that can result in lost samples and the need to repeat syntheses.

$chemist willing to remove solvent from fraction$

Evaporation the Biotage way - Speed without bumps

In the Biotage organic synthesis workflow, solvent removal is performed by the V-10 Touch evaporator. Utilising rotation, a thin-film of sample is created that combined with a vacuum and heat makes the V-10 Touch able to remove even the stubborn solvents in minutes. As a result, the V-10 Touch is the fastest evaporator across the range of organic solvents. Short evaporation times compared to other traditional methods means that the V-10 Touch does not consume a lot of energy, making for a greener operation. The thin-film operation means that bumping does not occur, so the system runs perfectly unattended, and automation combined with a simple interface and optional carousel and liquid handling make the V-10 Touch incredibly simple to use, and capable of drying down even large volumes of liquid. The clever software also allows the evaporating to dryness of complex samples, such as a range of preparative HPLC fractions with variable solvent compositions.

Product groups

Biotage® V-10 Touch

Biotage® V-10 Touch rapidly dries samples dissolved in both aqueous and organic solvents. It easily evaporates HPLC fractions from purification, high boiling point solvents from synthesis and features a dry-down onto silica technique for easier dry load capabilities prior to purification.

Accelerating Drug Discovery Research at New Facilities

PeptiDream Inc. relocated their head office in August 2017 and established its new research center in the Kawasaki City International Strategic Center. The expanded facility is expected to spawn innovative new drugs. The base instrumentation is provided by Biotage.

Organic Chemistry - Products from Start to Finish

Biotage has developed tools for every step of the organic process, with the entire workflow in mind. This dedicated suite of products vastly expands the range of options to truly accelerate discoveries of new molecules for future innovations.

How do I purify my high boiling solvent reaction mixture using silica flash chromatography?

Many chemists today find they need to synthesize molecules at higher temperatures in order to force difficult reactions to proceed. Solvents such as DMF, DMSO, and NMP are commonly used in these reactions as they facilitate the use of the high reaction temperatures. However, the same attributes that make these chemicals attractive as reaction solvents make compound recovery from them very difficult, including flash column chromatography. These high boiling solvents are typically polar and pose a challenge if purification is to be accomplished with normal-phase silica.

Why do I have waves in my chromatogram?

Are you currently or have you ever had an issue where your chromatographic trace shows a sine-wave pattern while a compound is eluting? If so, there are several causes, and solutions, for this issue.

When using UV to detect eluting compounds it is critical that all solvents used in the method are fully miscible. If not, when the solvents are mixed and pass through the column, they can form emulsions. Emulsions will intermittently block UV light leading to a wavy trace.

Our Sr. Technical Specialist Bob Bickler will guide your in this blog post.

Other blocks in the workflow

Biotage, pioneers of flash chromatography, has an extensive range of instrumentation and flash consumables designed to make your chemistry more efficient, simpler and greener.

Flash Chromatography

Microwave heating is by far the superior choice for synthesizing novel compounds and can offer advantages other than just speed. Working at temperatures and pressures not attainable through traditional heating, it allows chemists to perform reactions otherwise not possible. Faster, Simpler, Greener.

Organic Synthesis

Biotage offer a range of manual, semi-automated and fully automated systems depending on your peptide synthesis needs. Whether you require a system that makes a single peptide or multiple peptides in parallel, we have an instrument to suit your requirements.

Peptide Synthesis