Catecholamines are biomarkers used for the detection of diseases such as hypertension, pheochromocytoma and neuroblastoma. The main target analytes are dopamine, epinephrine and norepinephrine which are traditionally analyzed using liquid chromatography with electrochemical detection.
This application note describes a mixed-mode weak cation exchange solid phase extraction protocol using EVOLUTE® EXPRESS WCX 96-well plates for the extraction of three catecholamines (epinephrine, norepinephrine and dopamine) from human plasma prior to LC-MS/MS detection, which allows low level quantification of the analytes.
EVOLUTE EXPRESS solid phase extraction products combine powerful EVOLUTE sorbent chemistry with enhanced ‘EXPRESS’ column components. EVOLUTE EXPRESS products dramatically improve flow characteristics, and enhance sample preparation productivity. By truly eliminating the need for column conditioning and equilibration, samples can be prepared using a simple, fast load-wash-elute procedure.
In the Eyes of a Forensic Chemist - Interview with Professor Sabra Botch-Jones
Strategic Collaboration between Biotage and Yingsheng on the Chinese Market for Clinical Mass Spectrometry Applications
New Ultra Convenient Solid Phase Extraction Disks from Biotage
Hydrolyze Urine Samples In-Situ - New Application Note
DryVap® Inline Concentration System