This application note describes the extraction of 49 drugs of abuse from whole blood, prior to UPLC-MS/MS analysis.

An effective and efficient ISOLUTE SLE+ protocol optimized the for 1 mL sample capacity column formats is described in this application note. The simple sample preparation procedure delivers clean extracts, good recoveries and RSD values and LLOQ from 10 ng/mL.

ISOLUTE® SLE+ Supported Liquid Extraction columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation.

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Analytes

• 2-OH-ethyl-flurazepam
• 6-MAM
• 7-amino-clonazepam
• 7-amino-flunitrazepam
• Alprazolam
• Amphetamine
• Benzoylecgonine
• Bromazepam
• Buprenorphine
• Clonazepam
• Cocaine
• Codeine
• Diazepam
• Dihydrocodeine
• EDDP
• Estazolam
• Fentanyl
• Flunitrazepam
• Flurazepam
• Hydrocodone
• Hydromorphone
• Ketamine
• Lorazepam
• LSD
• MDA
• MDEA
• MDMA
• Mephedrone
• Methadone
• Methamphetamine
• Midazolam
• Morphine
• Nitrazepam
• Norbuprenorphine
• Nordiazepam
• Norfentanyl
• Norketamine
• Oxazepam
• Oxycodone
• Oxymorphone
• PCP
• Temazepam
• THC-COOH
• Triazolam
• Zaleplone
• Zolpidem
• Zopiclone
• α-OH-alprazolam
• α-OH-triazolam