I got to take a tour of the undergraduate lab at California State University, San Marcos with Dr. Robert Lafe. His lab is one of the first to have the new Biotage® Selekt Flash Purification System paired with the Sfär columns. We discussed how his undergraduate researchers were impacted by the broadened capabilities and their opportunity to learn about new instruments, and how it has affected his own research in his goals to gain tenure at the university.
The newly released Biotage® Selekt flash chromatography instrument can be run at a maximum flowrate of 300 mL/min or a maximum pressure of 30 bar. These high flowrates and pressures enable a user to perform chromatography using not only dry-packed, single-use plastic flash columns containing small (≥20 μm) spherical silica particles, but also semi-preparative, slurry-packed HPLC columns for multiple use with smaller (≤20 μm) spherical silica particles.
Recently, the Thorn-Seshold Research Group at Ludwig Maximilian University of Munich (LMU) purchased a Biotage® Selekt flash purification system to help them with their work. Biotage caught up with two of the researchers, Markus Müller and Li Gao, both Ph.D. students, to hear about their experiences with the system.
Peptide purification via flash chromatography has recently been demonstrated as a viable alternative to the more standard HPLC methods currently utilized. Flash chromatography offers peptide chemists the advantage of significantly greater loading capacity reducing the overall purification time but with a compromise of decreased peak resolution. Herein we present several strategies that, when implemented, allow for very high purity peptide samples purified by flash chromatography.
In this customer case we talk to Florian Wimmer and Natalie Schunck, Ph.D. Students working in the field of chemical materials science at the Department of Chemistry in the University of Konstanz, Germany. Florian and Natalie are working with their recently purchased Biotage® Selekt system, which has proved to be important for their work.
When purifying molecules, maximizing your efficiency is critical to your daily work. For us at Biotage, helping you to make the most of your time is of paramount importance. That is why we have designed a lean and compact automated flash system with two column channels, so you can prepare channel two while channel one is running.
Die Flash-Chromatographie ist die bevorzugte Reinigungsmethode für organische Stoffe, Arzneimittel und Naturstoffe. In jüngster Zeit hat sie auch die Peptidchemie erobert, verfügt sie doch über die Fähigkeit, eine Vielzahl unterschiedlicher Verbindungen effizienter zu trennen, als dies mit anderen Vorreinigungsverfahren wie z. B. dem Ausfällen (Protein-Crash) oder der Flüssig-Flüssig-Extraktion möglich ist. Zur Herstellung reiner Verbindungen können Chemiker je nach dem gewünschten Reinheitsgrad auf eine Vielzahl unterschiedlicher Variablen zurückgreifen. In diesem Whitepaper möchten wir die Faktoren erläutern, die für eine erfolgreiche Aufreinigung mithilfe der Flash-Chromatographie kontrolliert werden müssen.
Flash chromatography, a staple component of medicinal chemistry workflow, consumes a lot of organic solvent (upwards of half a million liters annually in just North America). Most, of this organic waste is incinerated off-site, liberating CO2 into the atmosphere. Because of this environmental impact, many companies are instituting requirements to reduce organic solvent waste but leaving the implementation to their chemistry departments. In this poster, we describe several proven ways to reduce solvent use without sacrificing purification efficiency.
In this application note, we compare the amounts of a threecomponent mixture that can be separated on columns of the same size, 25 g, but with different Silica material: SNAP KP-Sil (average 50 μm irregular particles), Sfär Silica (60 μm spherical particles) and Sfär Silica HC (20 μm spherical particles) using a Biotage® Selekt instrument.
Flash purification involves a simple liquid chromatography technique » Method development uses TLC as a way of deciding the parameters for the separation » Isocratic separations are easiest to develop, but gradient separations are more powerful » Software in the Isolera helps with conversion of an isocratic separation to a gradient » It is possible with the Spektra software to run step gradients » Loading options are dependent on the column type » SNAP offers the most flexibility » Care must be taken to choose the best loading option to get good purifications
Synthetic chemistry is hard enough; purifying the reaction product can be even more challenging. If your reaction mixture shows you made your target but also many byproducts, what do you do? You can resynthesize using different reaction conditions, use advanced work-up procedures, or you can purify using high-performance flash chromatography.