On a crisp December day, I travelled up to Queens, New York to speak with Dr. Aaron Muth from St. John’s University about his work in cancer research with Biotage lab equipment and specifically the SNAP Bio cartridges. As an Assistant Professor of Medicinal Chemistry, Dr. Muth and the members of his research group use our equipment every day. We chatted about his research, students, and the needs related to tools and equipment.
On one of the top floors in the Baylor College of Medicine Neurological Research Institute, I visited the Center for Drug Discovery and learn from the Director and Medicinal Chemistry Leader, Dr. Justin Anglin, about muscle cachexia as well as their innovative projects focused on male contraceptives. We were able to take a tour of their state-of-the-art lab after which includes a full suite of Biotage products, something Dr. Anglin uses every day.
PeptiDream Inc. relocated their head office in August 2017 and established its new research center in the Kawasaki City International Strategic Center. The expanded facility is expected to spawn innovative new drugs. The base instrumentation is provided by Biotage. R&D Director Keichi Masuya and his staff elaborate on the development.
When it comes to polar organic compound purification, many chemists turn to normal-phase flash chromatography often utilizing dichloromethane and methanol as the eluting solvents. While this can work, it often can be challenging to optimize due to methanol’s high polarity and protic chemistry.
Peptide purification via flash chromatography has recently been demonstrated as a viable alternative to the more standard HPLC methods currently utilized. Flash chromatography offers peptide chemists the advantage of significantly greater loading capacity reducing the overall purification time but with a compromise of decreased peak resolution. Herein we present several strategies that, when implemented, allow for very high purity peptide samples purified by flash chromatography.
This brochure presents the extensive range of instrumentation for flash purification from Biotage. To complement these products Biotage also offers a complete range of flash consumables, including columns in a variety of sizes packed with irregular and spherical silica, making Biotage your one-stop partner for your flash purification needs. Keywords: Isolera™ Dalton, Isolera™ Spektra, 10 Isolera™ Spektra One and Four, Isolera™ Spektra LS (Large Scale), Isolera™ Prime, Isolera™ ELSD-1080, Biotage® Flash 75/150, Biotage® Flash 400
Ease of use is what stands out as the top feature of Isolera™ flash chromatography system for Professor Anna Bernardi, head of the synthetic organic chemistry research group at the University of Milan. Her current research goal: developing sugar-like molecules, called glycomimetics, for healthier life.
User Case: Kissei Pharmaceutical Co., Ltd. One of Japan’s innovative pharmaceutical companies uses Isolera™ flash purification systems and Biotage® Initiator+ microwave synthesizers in the development of new prescription drugs. Modern lab instruments contribute to efficient use of time and resources at Kissei Pharmaceuticals.
Die Flash-Chromatographie ist die bevorzugte Reinigungsmethode für organische Stoffe, Arzneimittel und Naturstoffe. In jüngster Zeit hat sie auch die Peptidchemie erobert, verfügt sie doch über die Fähigkeit, eine Vielzahl unterschiedlicher Verbindungen effizienter zu trennen, als dies mit anderen Vorreinigungsverfahren wie z. B. dem Ausfällen (Protein-Crash) oder der Flüssig-Flüssig-Extraktion möglich ist. Zur Herstellung reiner Verbindungen können Chemiker je nach dem gewünschten Reinheitsgrad auf eine Vielzahl unterschiedlicher Variablen zurückgreifen. In diesem Whitepaper möchten wir die Faktoren erläutern, die für eine erfolgreiche Aufreinigung mithilfe der Flash-Chromatographie kontrolliert werden müssen.
Flash chromatography is the primary technology for separating, purifying, and isolating both synthetic organic compounds and natural products. Until recently, automated flash purification systems were limited to user-selected detection and collection wavelengths to fractionate separated compounds from sample mixtures. In this application note we demonstrate how the advances in Isolera Spektra are used in the purification of a spinach extract.
Isolera Spektra provides definitive fraction purity assessment in both 2-D and 3-D prior to any post flash analysis. With this information chemists quickly know if a fraction contains a pure product suitable for further mass and structure confirmation or whether the impure fractions must be re-purified. This knowledge improves synthesis throughput and avoids any embarrassment of submitting impure fractions for analytical evaluation.
Elution of polar molecules in flash chromatography sometimes requires solvent additives, making purification more cumbersome. The Isolera™ family of flash purification instruments and Biotage® SNAP KP-NH cartridges significantly reduce the extra work involved.