This guide describes simple practical strategies, hints and tips designed to mitigate or avoid cross-well contamination or ‘cross talk’ in high throughput 96-well based sample preparation techniques. Includes a section on controlling evaporative crosstalk in urine specimens with high drug concentrations.
Biotage® SPE Dry 96 and SPE Dry 96 Dual Sample Concentrators are suitable for evaporation of microplate samples across a broad range of formats. By delivering heated gas above and below each well, SPE Dry 96 systems dry samples quickly maintaining tight temperature control at user selected settings. Designed for high throughput sample processing, SPE Dry systems have simple front panel controls and use removable gas delivery assemblies for fast adjusting and cleaning.
Evaporative crosstalk is well-to-well cross contamination during extract evaporation after SLE or SPE extraction in a 96 well plate. It is often observed with volatile analytes like methamphetamine and amphetamine, but it can also occur with other drugs and metabolites when urine samples have very high analyte concentrations. ASMS, 2020.
In all areas of analytical laboratory testing it is vital to ensure proper quality measures are in place to reduce or eliminate cross contamination between samples, which could result in false positive and/or false negative results. In many cases sample carryover in the LC/MS system is checked early on in the method development process. However, one area that can often be overlooked the sample preparation stage. This involves all aspects including pipetting, sample transfer, extraction protocol, evaporation and mixing steps. This poster examines various stages of the sample preparation process to determine the potential for cross contamination and present approaches to minimize and or eliminate the effect. This is demonstrated via a series of dye experiments combined with analyte testing using LC-MS/MS.
This poster compares the performance of manual processing to a novel automated sample preparation system prior to GC/MS or LC-MS/MS analysis in forensic toxicology applications. Emphasis is placed on the potential for 96-well cross contamination and strategies for its elimination. TIAFT 2016 SOFT 2016
2-Hydroxyethylflurazepam 2-OH-Et-Flurazepam 6-acetylmorphine (MAM) 7-Aminoclonazepam 7-Aminoflunitrazepam 96-well plate Alprazolam Amphetamine Amphetamines Benzodiazepines Bromazepam Buprenorphine Clinical Clonazepam Codeine Column Diazepam Dihydrocodeine (DHC) Drugs of Abuse EDDP English Estazolam Extrahera Fentanyl Flunitrazepam Flurazepam Forensic GC-MS Hydrocodone Hydromorphone Ketamine LC-MS/MS Lorazepam MDA MDEA MDMA Mephedrone Methadone Methamphetamine Midazolam Morphine Nitrazepam Norbuprenorphine Nordiazepam Norfentanyl Norketamine Opiates Opiates Oxazepam Oxycodone Oxymorphone Phencyclidine (PCP) Posters Temazepam Triazolam Urine Whole blood Zaleplone Zolpidem Zopiclone z-drugs α-OH Triazolam α-OH alprazolam
This poster demonstrates a rapid and reliable sample preparation method using Supported Liquid Extraction (ISOLUTE SLE+) to extract a suite of 30 hormone analytes from a hydrolyzed urine matrix. Single injection analysis by LC-MS/MS shows that matrix effects are eliminated by the ISOLUTE SLE+ protocol and that analyte recovery and sensitivity have excellent clinical utility. MSACL 2016
11-deoxycortisol 11-keto-androsterone 11-oxoetiocholanolone 11-β-hydroxyandrosterone 11β-hydroxyetiocholanolone 2-OH-estrone 2-methoxyestrone 3α-androstanediol 3β-androstanediol 4-OH-estrone 4-methoxyestrone 96-well plate Allo-THB Androsterone Clinical Corticosterone DHEA (dehydroepiandrosterone Dehydroepiandrosterone (DHEA) English Etiocholanolone Extrahera Hormone Hormones Hydrolysed urine Posters Steroid Steroid Hormones Steroid metabolite Steroids THB THF Testosterone α-THF
Recreational marijuana use is currently legal in eleven U.S. states. Fourteen additional states have decriminalized marijuana, but have not legalized it. It is increasingly important for laboratories to develop accurate testing methods for THC, and its related compounds, in biological matrices of those that may be driving or committing crimes under the influence of marijuana. Biotage provides simple and effective sample prep protocols for cleanup of blood, urine, and oral fluid samples that produce reliable results, and are automation compatible.