The compound 4-methylimidazole (4-MEI) is formed as a byproduct in some foods and beverages. Caramel coloring Type III and Type IV in beverages is one of the ingredients which may contain 4-MEI. Products that potentially contain 4-MEI include certain colas, beers, soy sauces, breads, coffee, ammoniated livestock feed and other products.
The compound 4 Methylimidazole (4-MeI) is made during the production of caramel color commonly found in foods and beverages, especially sodas. The Codex Alimentarius of the World Health Organization sets a limit of 250 mg/kg of 4-MeI. Also, the state of California has added 4-MeI to its Prop 65 list at an exposure level of 29 μg/day. This study looks at 4-MeI levels in sodas and malt sodas which are sold in and outside the state of California. The first section, included in this poster, describes the performance of automated solid phase extraction of 4-MeI using conventional HPLC/MS detection. ASMS 2018, San Diego, CA
Mycotoxin testing awareness has increased as countries involved in world trade of raw agriculture and processed consumer products rely on a safe global food supply. Several mycotoxins are naturally produced and monitored; however, aflatoxins, produced mainly by the Aspergillus species, are considered to be the most important mycotoxin group in the world’s food supply to monitor.
US EPA Method 1664 for Hexane Extractable Material (HEM) or Oil and Grease has allowed use of solid phase extraction (SPE) instead of liquid-liquid extraction (LLE) with hexane since 2007 and this has been widely adopted in the US. SPE is an equivalent extraction technique to LLE and produces the same n-hexane extract. The extract, similar to LLE, may contain residual water that must be treated properly and removed from the n-hexane extract.
This application note demonstrates that the Biotage(R) Horizon 4790 using Atlantic C18 disks can effectively extract hormone compounds from 1-L water samples in approx 40 mins.
11-ketotestosterone 17-alpha-estradiol 17-alpha-ethynylestradiol 17-beta-estradiol 4790 Androgens Androsterone Application Notes Diethylstilbestrol Dihydrotestosterone (DHT) Disk Endocrine disrupter English Environmental Epitestosterone Estradiol Estriol Estrogens Estrone GC-MS Hormones Mestranol Norethindrone Progesterone Steroid Hormones Steroid metabolite Surface water Testosterone Water
This application note demonstrates the use of automated solid phase extraction for a variety of pharmaceutical compounds in drinking water, wastewater and surface water. The criteria specified in US EPA Method 1694 was met, even at very low concentrations. For lower concentrations a larger sample of water may be processed.
4790 Acetaminophen Albuterol Albuterol Application Notes Atenolol Caffeine Carbamazepine Cotinine DEET Dehydronifedipine Diclofenac Diltiazem Diphenhydramine hydrochloride Disk Drinking water English Environmental Gemfibrozil Ground water Ibuprofen LC-MS TOF Lamotrigine Metoprolol Naproxen Pharmaceuticals Sulfadimethoxine Sulfamethoxazole Triclocarban Trimethoprim Waste water
Pesticides have gone far in increasing food supply, important in a world expecting to have 9.8 billion people by 2050. However, the mechanisms that make pesticides effective in eliminating pests can also cause harm to humans and animals. News stories continue to point to contamination through spills and exposure through the respiratory system.
Atlantic® ReadyDisks are solid phase extraction (SPE) disks, pre-assembled into disposable holders and are used for extracting organic and semivolatile organic compounds from aqueous matrices. The ReadyDisks are designed for single-use extractions with maximum speed and convenience in mind and eliminate the need to clean holders between extractions.
When a sample preparation technique calls for removal of compounds using SPE, it is standard practice to determine what concentrations will produce an acceptable linear range for the assay. Traditional linear regression is often performed manually using pipettes to generate an external standard curve that unknown samples are measured against.
This work demonstrates the performance of automated solid phase extraction (SPE) for the extraction of PAHs from surface water sampled from a nearby pond containing some particulate. An automated system (Biotage® Horizon 4790) with Atlantic® DVB disks, following the requirements of method EN 16691, was used.
4790 Anthracene Application Notes Benzo (b) fluoranthene Benzo (g,h,i) perylene Benzo (k) Fluoranthene Benzo(a)pyrene Disk EN16691 Analytes EN16691 Water Quality English Environmental Fluoranthene GC-MS Indeno (1,2,3-cd) Pyrene PAHs Polyaromatic Hydrocarbons (PAH) Surface water
This application note describes the automated solid phase extraction (SPE) and GC/MS analysis of whole water samples according to EN16691 for PAHs. Method EN 16691 is a recently developed method that uses solid phase extraction to isolate organic compounds from 1 L of water using a divinylbenzene (DVB) solid phase extraction disk.
4790 Anthracene Application Notes Benzo (a) Pyrene Benzo (g,h,i) perylene Benzo(b)fluoranthene Benzo(k)Fluoranthene Disk EN16691 Water Quality English Environmental Fluoranthene GC-MS Indeno (1,2,3-cd) Pyrene PAHs Polyaromatic Hydrocarbons (PAH) Water
This application note demonstrates the performance of automated disk-based solid phase extraction (SPE), using Biotage® Horizon 5000 automated extraction system and Atlantic® DVB SPE disks, for concentration of organochlorine pesticides from whole water, prior to GC/MS analysis, following the requirements of EN16693:2015.
1,2 4 trichlorobenzene 1,2,3 trichlorobenzene 1,3,5 trichlorobenzene 5000 Alachlor Aldrin Application Notes DDD DDE DDT Dieldrin Disk Drinking water EN16693 Water Quality EN16693:2015 Analytes Endosulfan Endrin English Environmental GC-MS Hexachlorobenzene Hexachlorobutadiene Isodrin Organochlorine pesticides (OCP) Pentachlorobenzene Water alpha HCH beta HCH delta HCH gamma HCH
Polycyclic aromatic hydrocarbons (PAHs) can be found in a number of water types, such as drinking water, surface water, ground water and wastewater. There are serval types of extraction techniques that can be implemented to remove them from water for measurement including liquid-liquid extraction (LLE), continuous liquid-liquid extraction (CLLE) and solid phase extraction (SPE). With LLE and CLLE there is little selectivity within the methods for difficult matrices or other compound interferences.
This study shows that the Biotage® 4790 Automated Extractor system, along with the Atlantic™ DVB disk, the DryDisk® separation membrane, and the DryVap® Concentrator System can be successfully used to analyze for Nonyl Phenol and Bisphenyl A in water samples. This sample preparation process is fully automated, requiring minimal human intervention, and allowing for reductions in sample processing costs.
Oil and Grease is an important general measurement that can be used to indicate water quality and help ensure consistent wastewater treatment plant operation. Automated solid phase extraction can help to make the process more reproducible and easier to fit into the laboratory workflow. The SPE-DEX 3100 offers reliable operation, even with highly particulated samples.
Oil and grease (n-hexane extractable material) is a general indicator of the amount of hydrocarbons in a water supply. The Biotage Horizon 3100 automates the entire extraction process, fully compliant with US EPA Method 1664A/B, ISO 11349 and Standard Methods 5520B.