Peptide chemists typically use solutions containing high percentages of trifluoracetic acid (TFA) mixed with small volumes of various scavengers. These solutions can be readily evaporated using Biotage® V-10 Touch using the “Volatile” method. Despite the presence of water and other high boiling scavengers, this method is most efficient.
The following document will assist you in the preparation of your laboratory for a successful installation of Biotage® Horizon 5000.
The newly released Biotage® Selekt flash chromatography instrument can be run at a maximum flowrate of 300 mL/min or a maximum pressure of 30 bar. These high flowrates and pressures enable a user to perform chromatography using not only dry-packed, single-use plastic flash columns containing small (≥20 μm) spherical silica particles, but also semi-preparative, slurry-packed HPLC columns for multiple use with smaller (≤20 μm) spherical silica particles.
The following document will assist you in the preparation of your laboratory for a successful installation of DryVap®.
The following document will assist you in the preparation of your laboratory for a successful installation of DryVap®. Please read and sign where indicated at the end of this document. Your signature validates that you have understood our requirements.
The SPE-DEX 5000 software controls all operations of the system. It has basic passwording that allows routine operators to access a selection of stored methods to run on the system. The administrator can access functions to set up each module and develop methods by modifying previous methods or by starting a new method. We will describe the two processes in more detail in this product note.
The SPE-DEX 3100 Extractor is controlled through a small hand-held controller tethered to the instrument. The color touch screen is easy to see from any angle and features large icons to show the status of each station in the unit at any time.
Removing water with a membrane rather than the older technique of passing the solvent through a column of sodium sulfate (Na2SO4) brings several advantages. The most important analytically is that the membrane will not adsorb analytes or contaminate the extract with matrix or other potential interferences.
Confidence is what you need before you separate your precious compound on a new brand of flash chromatography column. Predictably getting your compound in a pure state is what you want, and if you can get it faster and use less solvent all the better. After all, your project needs to be completed on time, and trying something new is not always appealing. In order to take your first steps toward gaining that confidence you can follow the same procedures we use here at Biotage to evaluate columns.
The tasks carried out during the Preventive Maintenance (PM) visit shall include servicing the listed components and a final check, which is based on factory acceptance and qualification tests. These two procedures constitute a Gilson Preventive Maintenance Plan.