Phosphatidylethanol is an alcohol biomarker with a high degree of specificity; blood concentration of PEth correlates to the amount of alcohol consumed. This application note describes the extraction of 3 common species of PEth from whole blood using ISOLUTE® SLE+ supported liquid extraction prior to HPLC-MS/MS analysis. The method is easily automated using the Biotage® Extrahera, and details of the automated procedure are included in the application note.
Whether you need targeted methods for analytes such as Vitamin D metabolites in serum, or methods suitable for extraction of a wide panel of drugs and metabolites from urine, sample preparation before analysis is essential. This compendium highlights a selection of clinical sample preparation applications from Biotage.
Alcohol metabolite Application Notes Biomarker Brochures Catecholamines Clinical English Pain Management Peptides Product Notes Steroid Hormones Steroid metabolite Thyroid Hormone Tobacco Specific Nitrosamines (TSNAs) Vitamin D Vitamins Water Soluble Vitamins
This application note describes the extraction of ethyl glucuronide and ethyl sulfate from human urine using ISOLUTE® NH2 solid phase extraction columns. The method has been applied real patient samples that had been previously analyzed with a validated referee method. The results of the orthogonal measurements agreed, to provide similar diagnostic values.