This method describes the clean-up of acrylamide from cooked foodstuffs, using a multimode retention mechanism (hydrophobic, cation and anion exchange) for retention of interfering food components. The analytical technique is LC-MS-MS. ISOLUTE, MULTIMODE, SPE, FOOD, FOOD SCARE
This application note was developed for the clean-up of a non-polar solvent extract of vegetable material. The methodology is applicable to a wide range of organochlorine and organophosphorus pesticides and a number of vegetable types. The analytes are relatively non-polar, and will not be strongly retained by polar/anion exchange sorbents.
This method was developed for the extraction of the tetrahydrocannabinol carboxylic acid metabolite (THC-COOH) from urine using a mixed non-polar and anion exchange retention mechanism. Typical recoveries of the analyte are > 75%. THC, THC-COOH, BIOTAGE, ISOLUTE, SPE, DOA, drugs of abuse
Biotage® PRESSURE+ manifolds deliver positive pressure, parallel processing for 96 well plates and column formats. The systems utilize a consistent, uniform flow of positive pressure to move both low and high viscosity liquids through SPE plates and columns. ISOLUTE SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation and greatly matrix effects associated with biofluid analysis. SLE+, SLE, Supported Liquid Extraction, positive pressure manifold, Pressure+96, Pressure+48
This method is applicable to the determination of Oil and Grease as defined by EPA Method 1664 as Hexane Extractable Material (HEM, also referred to as Total Oil and Grease) as well as Silica Gel Treated-Hexane Extractable Material (SGT-HEM, also referred to as Total Petroleum Hydrocarbons or TPH). EPA, Fractionation, EPA Method 1664, TPH, Total Petroleum Hydrocarbons
The ISOLUTE HCX series of mixed-mode SPE sorbents is based on a combination of strong cation exchange and non-polar chemistries. Basic drugs are retained by two primary retention mechanisms. This allows a rigorous interference elution regime to be used to elute interferences retained by either non-polar or cation exchange interactions alone. Only analytes with both non-polar and basic characteristics are extracted using the ISOLUTE HCX series of sorbents, providing an extremely pure final extract.
ISOLUTE HAX mixed-mode SPE sorbent is based on a combination of strong anion exchange and non-polar chemistries. Acidic drugs are retained by two primary retention mechanisms. This allows a rigorous interference elution regime to be used to elute interferences retained by either non-polar or anion exchange interactions alone. Only analytes with both non-polar and acidic characteristics are extracted usign the ISOLUTE HAX sorbent, providing an extremely pure final extract.
ISOLUTE® HCX columns are based on cation exchange and C8 mixed mode chemistries. Basic drugs are therefore retained by two primary retention mechanisms - ionic and non-polar. This allows a more rigorous interference elution regime to be used, leading to a very clean final extract, as many non-polar interferences which are retained by a non-polar interaction alone, can be eluted selectively, prior to elution of the drug.
ISOLUTE® SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquidliquid extraction (LLE) for bioanalytical sample preparation, extracting up to 10 mL of aqueous sample. ISOLUTE SLE+ provides high analyte recoveries, eliminates emulsion formation and reduces sample preparation time by half.
Biotage® PRESSURE+ manifolds deliver positive pressure, parallel processing for 96 well plates, 1 mL 3 mL and 6 mL column formats. The systems utilize a consistent, uniform flow of positive pressure to move both low and high viscosity liquids through SPE plates and columns. Each port of the PRESSURE+ manifold independently maintains constant pressure, increasing the overall reproducibility of analyte recoveries. This unique design allows for partially populated racks to be used without sacrificing extraction efficiency.
This application note describes the extraction and clean-up of mineral oil (petroleum oil) from water samples according to ISO 9377-2:2000. This method involves a liquid-liquid extraction step followed by clean up using an SPE column containing layers of sodium sulfate and florisil, to remove halogenated and other polar interferences from the extract.
This Chemistry Data Sheet provides procedures for both the automated and manual fractionation of EPH in soil. The automated procedure is described on page 2 and the manual procedure is outlined on page 3. Chromatograms illustrate the ability of ISOLUTE EPH to provide efficient fractionation of hexane or pentane soil extracts into aliphatic and aromatic fractions (see Appendix).