Part No: PPS443.V.1Issued year: 2019File size: 2.98mbFile type: pdf
Analysis of drug panels in urine samples can be challenging, and the trend towards larger panels including multiple drug classes compounds the issues faced during method development.
This white paper examines a number of aspects of sample preparation, and their impact on the success of subsequent LC-MS/MS analysis of broad urine panels.
Section 1 examines the applicability of various sample preparation techniques: supported liquid extraction, reverse phase SPE and mixed-mode SPE, to the various classes of drugs extracted. In addition, hydrolysis approaches: enzyme type and protocol used (time, temperature), are compared.
Mixed-mode reverse phase/cation exchange SPE is widely used for extraction of basic drug classes from urine, but the inclusion of drugs and metabolites that exhibit ‘non-typical’ functionality within urine panels can be problematic. Section 2 examines the impact of various parameters (interference wash strength, elution solvent composition) on analyte retention, elution and extract cleanliness with particular focus on zwitterionic (gabapentin, pregabalin) and non-ionic (carisoprodol, meprobamate) drugs.
Part No: AN891.V.3Issued year: 2019File size: 4.41mbFile type: pdf
This application note describes the extraction of a panel of 19 steroid hormones from human serum using EVOLUTE® EXPRESS ABN solid phase extraction plates prior to LC/MS analysis. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs lower than 10% for the majority of analytes. Linearity of >0.99 is achieved for all analytes in the range 5–5000 pg/mL.
The application note includes details on automation of the extraction method using Biotage® Extrahera.
Part No: PPS540Issued year: 2019File size: 0.68mbFile type: pdf
The SmartPrep II Cartridge System is
designed for simple automation of
manual solid phase extraction (SPE)
methods. Just like manual SPE, up to
12 samples per SmartPrep II System
can be run in one batch.
Part No: PPS541Issued year: 2019File size: 0.56mbFile type: pdf
Oil and grease (n-hexane extractable
material) is a general indicator of the
amount of hydrocarbons in a water
supply. The Biotage Horizon 3100 automates the entire extraction
process, fully compliant with US EPA Method
1664A/B, ISO 11349 and Standard Methods 5520B.
Part No: PPS516Issued year: 2019File size: 0.12mbFile type: pdf
The Biotage® Horizon SPE-Z Prep Manifold (PN SPZ-0250) is
designed for high productivity disk solid phase extraction (SPE)
work. The manifold will allow you to use high-quality Pacific
O&G and Atlantic solid phase extraction (SPE) disks from
Horizon Technology in an easy-to-use three-position unit. The
system offers the flexibility to choose optimal size disks (47 or
100 mm) and pre-filters to process a wide range of water sample
Part No: PPS530Issued year: 2019File size: 0.24mbFile type: pdf
Oil and Grease is an important general measurement that can be used to indicate water quality and help ensure consistent wastewater treatment plant operation.
Automated solid phase extraction can help to make the process more reproducible and easier to fit into the laboratory workflow. The SPE-DEX 3100 offers reliable operation, even with highly particulated samples.
Part No: PPS539Issued year: 2019File size: 0.25mbFile type: pdf
Why spend hours using traditional evaporation techniques when the automated evaporation capability of the DryVap System can take your compound to
dryness in minutes? Through the precise application of vacuum, heat and nitrogen sparge, the DryVap System gently and predictably evaporates all residual solvent from your compound allowing you to quickly move on to the next step.
Part No: PPS515Issued year: 2019File size: 0.23mbFile type: pdf
The DryDisk membrane drying system provides advantages for removing water from nonpolar solvents, important in protecting the chromatograph in the analysis step, especially critical in protecting GC and GC/MS.