Vial preparation: 1) Remember to add stir bar for efficient mixing. Catalysts, salts, or visible precipitate should be washed clear of head space and into solution. Particles adhering to glass head space could cause excessive heating increasing possibility of failure of vial. Stay within specified vial volume range (see diagram for proper filling).

Manuals & User Guides

Laboratory chemical Not for therapeutic or diagnostic use

English Material Safety Data Sheets

VacMasterTM-96 Sample Processing Manifold for 96-well sample preparation applications

Product Notes

Certificates

This document will assist customers in the preparation of their laboratory for a successful installation of Biotage® Extrahera™.

Manuals & User Guides

Analysis of drug panels in urine samples can be challenging, and the trend towards larger panels including multiple drug classes compounds the issues faced during method development. This white paper examines a number of aspects of sample preparation, and their impact on the success of subsequent LC-MS/MS analysis of broad urine panels. Section 1 examines the applicability of various sample preparation techniques: supported liquid extraction, reverse phase SPE and mixed-mode SPE, to the various classes of drugs extracted. In addition, hydrolysis approaches: enzyme type and protocol used (time, temperature), are compared. Mixed-mode reverse phase/cation exchange SPE is widely used for extraction of basic drug classes from urine, but the inclusion of drugs and metabolites that exhibit ‘non-typical’ functionality within urine panels can be problematic. Section 2 examines the impact of various parameters (interference wash strength, elution solvent composition) on analyte retention, elution and extract cleanliness with particular focus on zwitterionic (gabapentin, pregabalin) and non-ionic (carisoprodol, meprobamate) drugs.

11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH) 6-acetylmorphine (MAM) 7-Aminoclonazepam 96-well plate Alprazolam Amitriptyline Amphetamine Amphetamines Anaesthetic Anticonvulsant Application Notes Barbiturates Benzodiazepines Benzoylecgonine Buprenorphine Butalbital Cannabinoid Carbamate hypnotic Carbamate muscle relaxant Carbamates Carisoprodol Chlordiazepoxide Clinical Clonazepam Cocaine Cocaines Codeine Diazepam Dihydrocodeine (DHC) EDDP English Fentanyl Forensic Gabapentin Hallucinogen Hydrocodone Hydrolysed urine Hydromorphone Ketamine LC-MS/MS Lorazepam MDMA Meperidine Meprobamate Methadone Methamphetamine Morphine N-des-tapentadol Naloxone Norbuprenorphine Nordiazepam Norfentanyl Norhydrocodone Norketamine Normeperidine Noroxymorphone Norpropoxyphene Nortryptiline O-desmethyltramadol Opiates Opioid Opioid agonist Oxazepam Oxycodone Oxymorphone Pentobarbital Phencyclidine Phencyclidine (PCP) Phenobarbital Pregabalin Product Notes Ritalinic acid Secobarbital Stimulants Sympathomimetic amine THC metabolites THC-COOH Tapentadol Temazepam Tramadol Tricyclic Antidepressant White Papers Zolpidem Zolpidem-phenyl-4-COOH z-drugs α-OH alprazolam

This application note describes the extraction of a panel of 19 steroid hormones from human serum using EVOLUTE® EXPRESS ABN solid phase extraction plates prior to LC/MS analysis. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs lower than 10% for the majority of analytes. Linearity of >0.99 is achieved for all analytes in the range 5–5000 pg/mL. The application note includes details on automation of the extraction method using Biotage® Extrahera.

11-deoxycorticosterone 11-deoxycortisol 17-OH-Pregnenolone 17-alpha-hydroxyprogesterone 18-OH-Corticosterone 21-deoxycortisol 96-well plate Aldosterone Androstenedione Application Notes Clinical Corticosterone Cortisol Cortisone DHEA (dehydroepiandrosterone DHEAS Dehydroepiandrosterone (DHEA) Dihydrotestosterone (DHT) English Estradiol Estrogens Estrone Extrahera LC-MS/MS Pregnenolone Progesterone Serum Steroid Hormones Steroid metabolite

Biotage Horizon 5000 Automated Extraction System is a programmable, multipurpose SPE system, capable of processing aqueous samples directly from their original containers.

Brochures

The SmartPrep II Cartridge System is designed for simple automation of manual solid phase extraction (SPE) methods. Just like manual SPE, up to 12 samples per SmartPrep II System can be run in one batch.

Brochures

Oil and grease (n-hexane extractable material) is a general indicator of the amount of hydrocarbons in a water supply. The Biotage Horizon 3100 automates the entire extraction process, fully compliant with US EPA Method 1664A/B, ISO 11349 and Standard Methods 5520B.

Brochures

The XcelVap® Automated Evaporation System is a modern compact benchtop laboratory product that provides rapid, gentle evaporation of up to 54 sample extracts ranging from 1.5 mL up to 200 mL each.

Brochures

Oral fluid is of increasing importance as a biological matrix in drugs of abuse and related analyte testing because of the ease of sample collection, and difficulty in adulteration of samples, particularly when compared with urine.

Application Notes Drugs of Abuse English Forensic LC-MS/MS Oral fluid Saliva White Papers

Biotage® Horizon 5000 is a three-position automated disk extraction system that conditions the disk, loads the sample (40 mL to 2 L) and elutes the analytes all without intervention.

Product Notes

Product Notes Sole Source/Lockout Specs

The Biotage® Horizon SPE-Z Prep Manifold (PN SPZ-0250) is designed for high productivity disk solid phase extraction (SPE) work. The manifold will allow you to use high-quality Pacific O&G and Atlantic solid phase extraction (SPE) disks from Horizon Technology in an easy-to-use three-position unit. The system offers the flexibility to choose optimal size disks (47 or 100 mm) and pre-filters to process a wide range of water sample

Product Notes

Product Notes Sole Source/Lockout Specs

Compounds, Horizon disks, methods and applications.

Manuals & User Guides Product Notes

Product Notes Sole Source/Lockout Specs

Oil and Grease is an important general measurement that can be used to indicate water quality and help ensure consistent wastewater treatment plant operation. Automated solid phase extraction can help to make the process more reproducible and easier to fit into the laboratory workflow. The SPE-DEX 3100 offers reliable operation, even with highly particulated samples.

Product Notes