Part No: P144Issued year: 2016File size: 0.6mbFile type: pdf
The ability to extract a broad range of different drugs from a biological matrix allows for the expedited analysis of a patient sample using LC-MS/MS. Typically small molecules are extracted from matrices like urine based on their polarities. A fast and reliable sample preparation method that could be implemented to extract drugs of different polarities from urine could be used as a screening tool to quickly identify the presence of illicit drugs in patient samples using LC-MS-MS.
This poster demonstrates the utility of supported liquid extraction for the extraction of over 30 different acidic, basic and neutral drugs in urine prior to LC-MS/MS.
MSACL 2016
Part No: AN859Issued year: 2016File size: 0.93mbFile type: pdf
This application note describes the extraction of acidic, basic, and neutral drugs from urine for screening purposes using ISOLUTE® SLE+ supported liquid extraction plates prior to LC-MS/MS analysis.
Part No: AN886Issued year: 2017File size: 1mbFile type: pdf
This application note describes the extraction of 96 licit and illicit drugs of abuse from urine prior to UPLC-MS/MS analysis using EVOLUTE® HYDRO CX 96-well plates.
EVOLUTE® HYDRO CX plates offer an efficient way to perform hydrolysis in the well of the extraction plate. This method provides high analyte recovery, reduced extraction time due to the elimination of a sample transfer step as well as the elimination of the column conditioning and equilibration steps, and a reduced risk for sample carryover or cross-contamination due to the elimination of the sample transfer step.
Part No: PPS443.V.1Issued year: 2019File size: 2.98mbFile type: pdf
Analysis of drug panels in urine samples can be challenging, and the trend towards larger panels including multiple drug classes compounds the issues faced during method development.
This white paper examines a number of aspects of sample preparation, and their impact on the success of subsequent LC-MS/MS analysis of broad urine panels.
Section 1 examines the applicability of various sample preparation techniques: supported liquid extraction, reverse phase SPE and mixed-mode SPE, to the various classes of drugs extracted. In addition, hydrolysis approaches: enzyme type and protocol used (time, temperature), are compared.
Mixed-mode reverse phase/cation exchange SPE is widely used for extraction of basic drug classes from urine, but the inclusion of drugs and metabolites that exhibit ‘non-typical’ functionality within urine panels can be problematic. Section 2 examines the impact of various parameters (interference wash strength, elution solvent composition) on analyte retention, elution and extract cleanliness with particular focus on zwitterionic (gabapentin, pregabalin) and non-ionic (carisoprodol, meprobamate) drugs.
Part No: P153Issued year: 2016File size: 0.52mbFile type: pdf
In postmortem cases, where drugs or pesticides have been used for
their poisonous properties, traditional matrices such as urine and
whole blood may be inappropriate for qualitative and quantitative
analysis. As the site of metabolism for most drugs and toxins, the
liver may provide more insight to cause of death than other bodily
fluids.
This poster describes the use of ISOLUTE SLE+ supported liquid extraction columns to extract a range of drug and pesticide classes form homogenised liver using a simple, streamlined workflow.
SOFT 2016
Part No: P138Issued year: 2015File size: 0.82mbFile type: pdf
This poster demonstrates a fast, reliable protocol to extract multiple drug of abuse panels from whole blood using a common supported liquid extraction methodology. This benefits laboratory workflow where multiple assays are run each day, saving both worker hours and
consumable costs.
SOFT 2015
ASMS 2016
TIAFT 2016
Part No: P156Issued year: 2017File size: 0.23mbFile type: pdf
Most drugs are excreted in urine as glucuronide conjugates. Hydrolysis using a beta-glucuronidase enzyme to convert the metabolites to their “free” form for analysis increases sensitivity. Red abalone (Kura Biotech), abalone (Campbell Scientific), and recombinant (IMCSzyme) beta-glucuronidase enzymes were evaluated to determine which provided the most complete hydrolysis of glucuronide metabolites without effecting the overall recovery of non-conjugated compounds.
EVOLUTE EXPRESS CX 96-well plates were used to extract hydrolysed urine samples, and the impact of th enzymes was compared.
MSACL 2017, Palm Springs
SOFT 2017, Boca Raton
Part No: AN752Issued year: 2011File size: 0.3mbFile type: pdf
This method describes the use of ISOLUTE SLE+ supported liquid extraction 1 mL sample volume columns to extract a range of barbiturates from human urine. The analysis of these analytes was carried out by GC-MS. This simplified and efficient extraction method has significant analyte recoveries ranging from 103-108% with LOQs of 10 ng/mL and RSDs <10%.
Barbs, Barbiturates, SLE, SLE+, ISOLUTE, GCMS, DoA, Forensic, Work Place Drug Testing, Supported Liquid Extraction, GC
Part No: AN820Issued year: 2014File size: 1.7mbFile type: pdf
This application note describes the extraction of barbiturates from oral fluid matrix collected using the Intercept Oral Fluid Drug Test Kit(Orasure Technologies), prior to GC/MS analysis.
The ISOLUTE SLE+ protocol is optimized for 400 μL and 1 mL sample
capacity formats. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 90% with RSDs lower than 7% for all analytes.
Part No: AN868Issued year: 2016File size: 1.59mbFile type: pdf
This application note describes the extraction of barbiturates, using ISOLUTE SLE+ supported liquid extraction columns, from oral fluid matrix collected using the NeoSal collection device, prior to GC/MS analysis.
Part No: AN824Issued year: 2014File size: 1.25mbFile type: pdf
This application note describes the extraction of Butalbarbital, Butabarbital, Amobarbital, Pentobarbital,Secobarbital, Hexobarbital and Phenobarbital from oral fluid matrix collected using the Oral-Eze® collection devices, prior to GC/MS analysis.
Part No: AN821Issued year: 2014File size: 2.3mbFile type: pdf
This application note describes the extraction of
barbiturates from oral fluid matrix collected using Quantisal™ collection devices (Immunalysis), prior to GC/MS analysis.
The ISOLUTE SLE+ protocol is optimized for 400 μL and 1 mL sample
capacity formats. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 88% with RSDs lower than 6% for all analytes.
Part No: P111Issued year: 2014File size: 0.45mbFile type: pdf
This poster describes the extraction of barbiturates from oral fluid collected using common collection devices using ISOLUTE SLE+ Supported Liquid Extraction columns, optimised for GC-MS analysis.
SOFT 2014
Part No: IST1032AIssued year: 2011File size: 0.13mbFile type: pdf
The following method was developed for the extraction of barbiturate drugs from urine, including: butabarbital, amobarbital, cyclobarbital and phenobarbital. Typical recoveries for these drugs are > 85%.
ISOLUTE, BIOTAGE, HCX, DOA, Nervous System
Part No: AN853Issued year: 2015File size: 0.7mbFile type: pdf
This application note describes the extraction of barbiturate compounds from whole blood, prior to GC/MS analysis. This protocol also allows the simultaneous extraction of various other drugs of abuse classes: amphetamines, benzodiazepines, cocaine and opiates.
ISOLUTE® SLE+ columns with 1 mL sample capacity are used to extract whole blood samples following a straightforward sample dilution. No protein precipitation or other pre-treatment is required prior to sample loading. The sample preparation procedure delivers clean extracts, good recoveries and RSD values and LLOQs from 20 ng/mL (analyte dependant).
Part No: AN864Issued year: 2016File size: 2.43mbFile type: pdf
This application note describes the extraction of a broad range of analytes from liver tissue matrix prior to GC/MS analysis using ISOLUTE® SLE+ supported liquid extraction columns.
A protocol has been developed that allows the simultaneous
extraction of various drugs of abuse classes: amphetamines, barbiturates, benzodiazepines, cocaine, ketamine, THC.
In addition to these drug panels, simultaneous extraction of carbamate, organochlorine, organophosphate, pyrethroid, and triazine pesticide classes is demonstrated.
Part No: Issued year: 2014File size: 1.02mbFile type: pdf
Testing for drugs of abuse in oral fluids can strongly benefit the criminal justice field as a less invasive and cost-effective approach for drug detection when compared to blood or urine sampling. Oral fluid analysis has facilitated laboratory analysis for many drugs of abuse and is a constantly evolving analysis procedure which benefits from increasingly sensitive methods of detection. The Laser Diode Thermal Desorption (LDTD) source combined with Mass spectrometry is presented as a new screening tool for drug analysis in Oral Fluids. Analysis speed of LDTD provides accurate results in seconds in combination with exceptional specificity of MS instruments make a powerful platform for the screening of different drugs of abuse and new emerging drugs. Different extraction procedures are available; however those methods depend on specific drug conditions: basic or acid drugs / hydrophilic or hydrophobic. A new extraction approach, Supported Liquid Extraction (SLE+) is evaluated as generic extraction procedure.
MSACL 2014
Part No: P165Issued year: 2017File size: 0.32mbFile type: pdf
This poster evaluates 3 different sample preparation approaches (ISOLUTE SLE+, EVOLUTE EXPRESS ABN, EVOLUTE EXPRESS CX) for extraction of large multi-drug urine panels.
Each approach is assessed in terms of suitability for extraction of analytes with different different properties (pka, LogP etc).
ASMS 2017
Part No: P153.v.1Issued year: 2017File size: 1.12mbFile type: pdf
This poster describes a simple but effective method for screening a range of pesticides and drugs of abuse from liver samples. Liver tissue is homogenized using the Biotage® Lysera, and the subsequent extract is cleaned up using ISOLUTE SLE+ supported liquid extraction columns.
ASMS 2017