Part No: P047Issued year: 2012File size: 0.6mbFile type: pdf
The potential harm caused by synthetic cannabinoids is a significant concern since exposures and reports of human fatalities due to these substances is widespread . Detection of these relatively new type designer drugs in blood is essential in forensic toxicology, other scientific and medical fields.
Cannabinoids, SLE, SLE+, Supported Liquid Extraction,
Part No: AN793Issued year: 2013File size: 2.04mbFile type: pdf
This application note describes the extraction of a range of SPICE drugs and metabolites in urine which are
typically screened in forensic toxicology panels using ISOLUTE® SLE+ in a 96-well plate format. Both manual
(Biotage Pressure+ 96) and automated (TECAN Freedom EVO® 100) processing conditions are described.
Part No: AN774Issued year: 2012File size: 0.19mbFile type: pdf
This application note describes the extraction and quantitation of Cannabimimetic Naphthoylindoles (Synthetic Cannabinoids) and their metabolites (JWH Series) from various matrices using Supported Liquid Extraction (SLE). Synthetic Cannabinoids or SPICE as they are commonly known have become an increasing problem as one of the newest forms of illicit drugs being consumed today. These compounds bind to the cannabinoid receptors in mammals triggering similar euphoric symptoms as Tetrahydrocannabinoids (THC). Currently robust and fast analytical methods of analysis are required to aid in the screening and detection of this growing class of compounds. The recoveries obtained for the synthetic cannabinoids parent and metabolites ranged from 70-98 %.
Supported Liquid Extraction, SLE, SLE+, SPICE, Synthetic Cannabinoids, JWH, metabolites,
Part No: AN791Issued year: 2013File size: 3.25mbFile type: pdf
This application note describes the extraction of a range of SPICE drugs and metabolites from neat oral fluid and oral fluid from a commercial collection kit using ISOLUTE® SLE+ in both 96-well plate and column formats.
SPICE, oral fluid, Intercept Orasure, LC-MSMS, SLE
Part No: P075Issued year: 2014File size: 0.66mbFile type: pdf
Drug of abuse testing is typically conducted using urine specimens from patients being screened for illicit drug use. While collection of urine is considered a relatively non-invasive drug testing action, it is not convenient for law enforcement to do in the field. The ability to collect an oral fluid sample in the field can be considered non-evasive and simple to implement. Currently there are oral fluid collection kits (e.g. Immunalysis Quantisal™, Orasure Technologies Intercept®) that facilitate the collection in easy to follow protocols. Oral fluid samples collected using standard collection kits can be qualitatively and
quantitatively analyzed by LC-MS/MS, post extraction of the target analytes from the matrix. Drug analytes can be successfully extracted from oral fluid using Supported Liquid Extraction (ISOLUTE SLE+), which offers an efficient alternative to traditional liquid-liquid extraction (LLE). In this poster, we
demonstrate a new rapid and reliable sample preparation method to extract a broad suite of drugs (Figure 1) from neat oral fluid and buffered oral fluid matrices.