Part No: AN099-HORIssued year: 2014File size: 2.06mbFile type: pdf
Aflatoxins, a mold largely produced by Aspergillus flavus and Aspergillus parasiticus1 are commonly tested mycotoxins found naturally in a wide range of agriculture crops and food products. Due to their harmful effects on human health, animal health, and global trade, aflatoxins are regulated in most countries and have established global limits in a wide variety of matrices.
Part No: AN097-HORIssued year: 2014File size: 2.28mbFile type: pdf
Deoxynivalenol is a common mycotoxin found in wheat agriculture crops. Being the most popular agricultural cereal crop, wheat is also a dietary staple for about 35% of the global human population and animals. Safety of wheat food and agriculture products is a concern, with deoxynivalenol present in 90% of food and feed samples.
Part No: AN057-HORIssued year: 2010File size: 0.72mbFile type: pdf
At present, pollution of freshwater algae has become aglobal environmental problem. Of all the different pollution types, microcystin LR is the most toxic and the most acute hazard as far as is known presently
Part No: AN036-HORIssued year: 2009File size: 0.86mbFile type: pdf
The purpose of this application note is to demonstrate the capabilities of the Biotage® Horizon 4790 Automated Extractor System when used for the analysis of nitramine, nitroaromatic, and nitrate ester compounds in surface and ground water.
Part No: AN096-HORIssued year: 2014File size: 2.89mbFile type: pdf
Mycotoxin testing in consumer food products has become increasingly important as global food trade increases, making it necessary to identify mycotoxins efficiently and accurately. Deoxynivalenol, also known as vomitoxin, is a mycotoxin commonly found in wheat feed crops. Because wheat is a highly-used raw agricultural export commodity in many counties for both animal feed and consumer food products, it is an important component in the diets of both humans and animals.
Part No: IST1071AIssued year: 2011File size: 0.17mbFile type: pdf
This method details the use of a non-polar retention mechanism for the extraction of catecholamines (noradrenaline, adrenaline, dopamine) from urine. The polar analytes form a non-polar complex with phenylboronic acid and are retained on the non-polar MFC18 column. Typical recoveries are >85%.
ISOLUTE, Catecholamines, dopind, sports drug testing,
Part No: AN709Issued year: 2010File size: 0.09mbFile type: pdf
The following methods have been developed for the selective extraction of clenbuterol from calf urine. The method is highly reproducible and offers clenbuterol recoveries of ≥ 70%.
MIP, Molecularly Imprinted Polymers, AFFINILUTE, SupelMIP,
Part No: IST1041AIssued year: 2011File size: 0.14mbFile type: pdf
This method was developed for the extraction of lysergic acid diethylamide (LSD) from whole blood using a mixed non-polar and cation exchange retention mechanism.
LSD, BIOTAGE, ISOLUTE, HCX, DOA, Hallucinogen
Part No: AN701Issued year: 2007File size: 0.07mbFile type: pdf
This procedure is recommended for the extraction of a variety of pharmaceuticals from water using non-polar polymeric SPE. These pharmaceuticals were identified as potential environmental contaminants by the Environment Agency.
Pharmaceuticals, waste water, antibiotics, EVOLUTE,
Part No: AN716Issued year: 2011File size: 0.14mbFile type: pdf
The following method has been developed for the extraction of the following triazines and triazine metabolites; atrazine, simazine, propazine, cyanazine, sebuthylazine deisopropylatrazine, deethylatrazine, deethylterbutylazine, prometon and hydroxyterbutylazine from water. Recoveries are in the range 80-95% and are reproducible, allowing sharp decision limits. The maximum accepted level of triazines in drinking water is 0.1 μg/L (EU legislation).
AFFINILUTE, MIP, SupelMIP, Molecularly Imprinted Polymer
Part No: AN084-HORIssued year: 2012File size: 1mbFile type: pdf
Within EPA Method 549.2, the suggested rate to load a sample onto a solid phase cartridge is given as 3 to 6 mL/min.
This Application Note outlines the process used to extract diquat and paraquat from water samples using a faster loading rate than given by the EPA in method 549.2
Part No: P204Issued year: 2019File size: 0.44mbFile type: pdf
Flash Chromatography is the primary tool used by medicinal chemists for reaction mixture purification. Over the past 10 or so years, the use of reversed-phase flash chromatography for reaction mixture purification has increased dramatically due to both chromatographic and non-chromatographic reasons.
Part No: AN119-HORIssued year: 2010File size: 1.39mbFile type: pdf
US EPA method 550.1 is a method used by regulatory agencies to determine PAH’s in drinking water by liquid-solid extraction (LSE) and high performance liquid chromatography (HPLC).1 The method outlines the steps needed to perform extractions of PAHs from drinking water sources and finished drinking water; as well as the quality control (QC) program requirements to ensure the method is operated under control.
Use of Biotage automated sample preparation products allows users to achieve the precise and consistent data needed to comply with EPA method 550.1 QC specifications as stated in method, section 10.3.2., for Initial Demonstration of Capability (IDC); while at the same time, streamlining laboratory practices to increase lab productivity.
Part No: AN042-HORIssued year: 2009File size: 0.81mbFile type: pdf
This application note discusses the preliminary findings and steps taken to develop an SPE method used to detect the lower limit of 50 ng/L of Bentazone in influent river water, using the BiotageHorizon 4790
Part No: P077Issued year: 2014File size: 0.48mbFile type: pdf
This poster uses gel electrophoresis data to visually illustrate the amount of residual protein in extracts prepared using protein precipitation, supported liquid extraction and various silica and polymer based SPE approaches.
Part No: AN066-HORIssued year: 2010File size: 1.39mbFile type: pdf
The extraction of samples for specialized liquid chromatography (LC) applications is frequently challenged by the need for a very particular solvent, or mix of solvents which are required to achieve the correct chromatography. These methods typically extract the analytes of interest into a water soluble solvent such as methanol and concentrate the extracts to total dryness before reconstituting the residue in another solvent. The evaporation step can be done using commercially available concentration apparatuses such as water baths or nitrogen gas sparging. However, these require constant analyst intervention to prevent the residue from being carbonized or otherwise thermally degraded.
Part No: AN083-HORIssued year: 2012File size: 1mbFile type: pdf
This Application Note outlines the process used to extract diquat and paraquat from water samples using the Biotage Horizon SmartPrep Automated Solid Phase Cartridge Extractor, according to EPA method 549.2.