Traditionally the analysis of aldosterone and angiotensin (for plasma renin activity measurement) are performed separately. However, the relationship of the aldosterone-to-renin-ratio is a very useful tool to help define the cause of secondary hypertension. This poster compares sample preparation options for the simultaneous extraction of Angiotensin I, II and Aldosterone from plasma. ASMS 2018

96-well plate Aldosterone Angiotensin I Angiotensin II, Clinical English LC-MS/MS Peptides Plasma Posters Steroid Hormones

Traditionally the analysis of aldosterone and angiotensin (for plasma renin activity measurement) are performed separately. However, the relationship of the aldosterone-to-renin-ratio is a very useful tool to help define the cause of secondary hypertension. This poster compares sample preparation options for the simultaneous extraction of Angiotensin I, II and Aldosterone from plasma. MSACL EU 2018

96-well plate Aldosterone Angiotensin I Angiotensin II, Clinical English LC-MS/MS Peptides Posters Steroid Hormones

This application note describes the extraction of a panel of 19 steroid hormones from human serum using EVOLUTE® EXPRESS ABN solid phase extraction plates prior to LC/MS analysis. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs lower than 10% for the majority of analytes. Linearity of >0.99 is achieved for all analytes in the range 5–5000 pg/mL. The application note includes details on automation of the extraction method using Biotage® Extrahera.

11-deoxycorticosterone 11-deoxycortisol 17-OH-Pregnenolone 17-alpha-hydroxyprogesterone 18-OH-Corticosterone 21-deoxycortisol 96-well plate Aldosterone Androstenedione Application Notes Clinical Corticosterone Cortisol Cortisone DHEA (dehydroepiandrosterone DHEAS Dehydroepiandrosterone (DHEA) Dihydrotestosterone (DHT) English Estradiol Estrogens Estrone Extrahera LC-MS/MS Pregnenolone Progesterone Serum Steroid Hormones Steroid metabolite

This application note describes the extraction of a panel of steroid hormones from human serum using ISOLUTE® SLE+ Supported Liquid Extraction plates prior to LC/MS-MS analysis. The simple sample preparation procedure delivers clean extracts and high, reproducible recoveries (>75%, RSD <10% ) for all analytes in human serum, with linearity >0.99 in the range 5–5000 pg/mL.

11-deoxycorticosterone 11-deoxycortisol 17-OH-Pregnenolone 17-alpha-hydroxyprogesterone 18-OH-Corticosterone 21-deoxycortisol 96-well plate Aldosterone Androstenedione Application Notes Clinical Corticosterone Cortisol Cortisone DHEA (dehydroepiandrosterone DHEAS Dehydroepiandrosterone (DHEA) Dihydrotestosterone (DHT) English Estradiol Estrogens Estrone LC-MS/MS Pregnenolone Progesterone Serum Steroid Hormones Steroid metabolite Testosterone

This application note describes the extraction of testosterone and other steroid hormones from human plasma using ISOLUTE SLE+ supported liquid extraction plates (96-well) with LC-MS/MS analysis. This method describes the use of ISOLUTE SLE+ supported liquid extraction plates (96-well) to extract a range of steroid hormones including testosterone and progesterone from human plasma. This simplified and efficient extraction method has significant analyte recoveries ranging from 90-107% withb LOQs as low as 500 pg/mL. ISOLUTE, TESTOSTERONE, SLE, SLE+, SUPPORTED LIQUID EXTRACTION, STEROID, HORMONE, PLASMA, ANDROGEN, CLINICAL

11-deoxycortisol 17-alpha-hydroxyprogesterone 21-deoxycortisol Aldosterone Androstenedione Androsterone Application Notes Clinical Dehydroepiandrosterone (DHEA) LC-MS/MS Plasma Progesterone Steroid Hormones Testosterone

This application note describes the simultaneous extraction of aldosterone at low (< 25pg/mL) concentration and angiotensin I and II from plasma, with the potential to improve throughput and reduce time and costs in PRA determination.

96-well plate Aldosterone Angiotensin I Angiotensin II, Application Notes Clinical English Extrahera LC-MS/MS Peptides Plasma Steroid Hormones