Traditionally the analysis of aldosterone and angiotensin (for plasma renin activity measurement) are performed separately. However, the relationship of the aldosterone-to-renin-ratio is a very useful tool to help define the cause of secondary hypertension. This poster compares sample preparation options for the simultaneous extraction of Angiotensin I, II and Aldosterone from plasma. MSACL EU 2018 MSACL NA 2019

96-well plate Aldosterone Angiotensin I Angiotensin II, Clinical English Hormone Hormones LC-MS/MS Peptides Posters Steroid Steroid Hormones Steroids

This application note describes the extraction of a panel of 18 steroid hormones from horse hair using ISOLUTE® SLE+. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs lower than 5% for most analytes. Linearity of greater than 0.999 is achieved for all analytes in the range 0.5-500 pg/mg of hair.

11-deoxycorticosterone 11-deoxycortisol 17-alpha-hydroxyprogesterone 21-deoxycortisol 96-well plate Aldosterone Anabolic steroids Androstenedione Application Notes Corticosterone Cortisone DHEA (dehydroepiandrosterone Dehydroepiandrosterone (DHEA) Dihydrotestosterone (DHT) English Estradiol Estrone Forensic Hair Hormone Hormones Horse Hair LC-MS/MS Lysera Progesterone Steroid Steroid Hormones Steroid metabolite Steroids α-boldenone α-trenbolone β-boldenone β-trenbolone

This application note describes the extraction of a panel of 19 steroid hormones from human serum using EVOLUTE® EXPRESS ABN solid phase extraction plates prior to LC/MS analysis. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs lower than 10% for the majority of analytes. Linearity of >0.99 is achieved for all analytes in the range 5–5000 pg/mL. The application note includes details on automation of the extraction method using Biotage® Extrahera.

11-deoxycorticosterone 11-deoxycortisol 17-OH-Pregnenolone 17-alpha-hydroxyprogesterone 18-OH-Corticosterone 21-deoxycortisol 96-well plate Aldosterone Androstenedione Application Notes Clinical Corticosterone Cortisol Cortisone DHEA (dehydroepiandrosterone DHEAS Dehydroepiandrosterone (DHEA) Dihydrotestosterone (DHT) English Estradiol Estrogens Estrone Extrahera Hormone Hormones LC-MS/MS Pregnenolone Progesterone Serum Steroid Steroid Hormones Steroid metabolite Steroids

This application note describes the extraction of a panel of steroid hormones from human serum using ISOLUTE® SLE+ Supported Liquid Extraction plates prior to LC/MS-MS analysis. The simple sample preparation procedure delivers clean extracts and high, reproducible recoveries (>75%, RSD <10% ) for all analytes in human serum, with linearity >0.99 in the range 5–5000 pg/mL.

11-deoxycorticosterone 11-deoxycortisol 17-OH-Pregnenolone 17-alpha-hydroxyprogesterone 18-OH-Corticosterone 21-deoxycortisol 96-well plate Aldosterone Androstenedione Application Notes Clinical Corticosterone Cortisol Cortisone DHEA (dehydroepiandrosterone DHEAS Dehydroepiandrosterone (DHEA) Dihydrotestosterone (DHT) English Estradiol Estrogens Estrone Hormone Hormones LC-MS/MS Pregnenolone Progesterone Serum Steroid Steroid Hormones Steroid metabolite Steroids Testosterone

This application note describes the extraction of a panel of 19 steroid hormones from human urine using EVOLUTE® EXPRESS ABN solid phase extraction plates prior to LC-MS/MS analysis. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 75% with RSDs lower than 5% for most analytes. Linearity of greater than 0.999 is achieved for all analytes in the range 1–1000 pg/mL. Manual and automated (using Biotage Extrahera) extraction protocols are detailed.

11-deoxycorticosterone 17-OH-Pregnenolone 17-alpha-estradiol 17-alpha-hydroxyprogesterone 17-beta-estradiol 18-OH-Corticosterone 21-deoxycortisol 96-well plate Aldosterone Androstenedione Application Notes Clinical Corticosterone Cortisol Cortisone DHEA (dehydroepiandrosterone DHEAS Dihydrotestosterone (DHT) English Estrone Hormone Hormones LC-MS/MS Pregnenolone Progesterone Steroid Steroid Hormones Steroid metabolite Steroids Testosterone Urine

This application note describes the extraction of a panel of 19 steroid hormones from human urine using ISOLUTE® SLE+ Supported Liquid Extraction plates prior to LC/MS-MS analysis. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 90% with RSDs lower than 10% for all analytes. Linearity of greater than 0.999 is achieved for all analytes in the range 1-1000 pg/mL. Manual and automated (using Biotage Extrahera) extraction protocols are detailed.

11-deoxycorticosterone 11-deoxycortisol 17-OH-Pregnenolone 17-alpha-hydroxyprogesterone 18-OH-Corticosterone 21-deoxycortisol 96-well plate Aldosterone Androstenedione Application Notes Clinical Corticosterone Cortisol Cortisone DHEA (dehydroepiandrosterone DHEAS Dehydroepiandrosterone (DHEA) English Estradiol Estrone Extrahera Hormone Hormones LC-MS/MS Pregnenolone Progesterone Steroid Steroid Hormones Steroid metabolite Steroids Testosterone Urine

This application note describes the extraction of testosterone and other steroid hormones from human plasma using ISOLUTE SLE+ supported liquid extraction plates (96-well) with LC-MS/MS analysis. This method describes the use of ISOLUTE SLE+ supported liquid extraction plates (96-well) to extract a range of steroid hormones including testosterone and progesterone from human plasma. This simplified and efficient extraction method has significant analyte recoveries ranging from 90-107% withb LOQs as low as 500 pg/mL. ISOLUTE, TESTOSTERONE, SLE, SLE+, SUPPORTED LIQUID EXTRACTION, STEROID, HORMONE, PLASMA, ANDROGEN, CLINICAL

11-deoxycortisol 17-alpha-hydroxyprogesterone 21-deoxycortisol Aldosterone Androstenedione Androsterone Application Notes Clinical Dehydroepiandrosterone (DHEA) Hormone Hormones LC-MS/MS Plasma Progesterone Steroid Steroid Hormones Steroid metabolite Steroids Testosterone

This application note describes the simultaneous extraction of aldosterone at low (< 25pg/mL) concentration and angiotensin I and II from plasma, with the potential to improve throughput and reduce time and costs in PRA determination.

96-well plate Aldosterone Angiotensin I Angiotensin II, Application Notes Clinical English Extrahera LC-MS/MS Peptides Plasma Steroid Hormones