Part No: P144Issued year: 2016File size: 0.6mbFile type: pdf
The ability to extract a broad range of different drugs from a biological matrix allows for the expedited analysis of a patient sample using LC-MS/MS. Typically small molecules are extracted from matrices like urine based on their polarities. A fast and reliable sample preparation method that could be implemented to extract drugs of different polarities from urine could be used as a screening tool to quickly identify the presence of illicit drugs in patient samples using LC-MS-MS.
This poster demonstrates the utility of supported liquid extraction for the extraction of over 30 different acidic, basic and neutral drugs in urine prior to LC-MS/MS.
Part No: P198.V.1Issued year: 2019File size: 0.59mbFile type: pdf
The low-level detection required for cannabis use combined with the complexity of hair testing makes for a challenging application. This poster aims to demonstrate simplified sample preparation workflow for low level analysis of THC and metabolites from hair, using Biotage Lysera and ISOLUTE SLE+ columns for sample preparation.
Part No: AN815Issued year: 2014File size: 1.52mbFile type: pdf
The method described in this application note achieves high recoveries of THC and an extended suite of common metabolites in oral fluid from Quantisal (Immunalysis) oral fluid collection devices.
This application note describes effective and efficient
ISOLUTE SLE+ protocols optimized for sample loading volumes of either 300 μL or 800 μL. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 64% with RSDs of <10% for all analytes.
Part No: P153Issued year: 2016File size: 0.52mbFile type: pdf
In postmortem cases, where drugs or pesticides have been used for
their poisonous properties, traditional matrices such as urine and
whole blood may be inappropriate for qualitative and quantitative
analysis. As the site of metabolism for most drugs and toxins, the
liver may provide more insight to cause of death than other bodily
This poster describes the use of ISOLUTE SLE+ supported liquid extraction columns to extract a range of drug and pesticide classes form homogenised liver using a simple, streamlined workflow.
Part No: P194.V.2Issued year: 2020File size: 1.09mbFile type: pdf
Hair analysis is growing in popularity due to the non-invasive nature of the sample collection. Although not used as routinely as other matrices such as blood or urine it does have advantages in that the matrix can indicate prolonged drug exposure. NCFM 2020, Reykjavik, Iceland
Part No: P194Issued year: 2019File size: 1.08mbFile type: pdf
Sample preparation for hair analysis is often lengthy involving multiple manual labor steps from cutting, washing, homogenization/pulverization, digestion, sample extraction and analysis. This poster aims to demonstrate a streamlined sample
preparation workflow for hair analysis.
The workflow is evaluated using a suite of drugs of abuse, including THC and metabolites.
MSACL NA 2019
Part No: AN864Issued year: 2016File size: 2.43mbFile type: pdf
This application note describes the extraction of a broad range of analytes from liver tissue matrix prior to GC/MS analysis using ISOLUTE® SLE+ supported liquid extraction columns.
A protocol has been developed that allows the simultaneous
extraction of various drugs of abuse classes: amphetamines, barbiturates, benzodiazepines, cocaine, ketamine, THC.
In addition to these drug panels, simultaneous extraction of carbamate, organochlorine, organophosphate, pyrethroid, and triazine pesticide classes is demonstrated.
Part No: AN919Issued year: 2019File size: 2.54mbFile type: pdf
This application note describes a procedure for sample pre-treatment and extraction of THC and metabolites from human hair, using Biotage® Lysera for matrix pulverization of the sample prior to clean up using ISOLUTE® SLE+ supported liquid extraction.
Manual processing protocols were developed using the Biotage® PRESSURE+ 96 (plate format) or 48 (column format) positive pressure manifolds. For automated processing, protocols were developed using Biotage® ExtraheraTM.
Part No: AN929Issued year: 2020File size: 0.86mbFile type: pdf
This application note describes a procedure for sample pre-treatment and extraction of THC and its metabolites from human nail samples, using the Biotage® Lysera for matrix pulverisation, and pre concentration of the sample prior to clean up using ISOLUTE® SLE+ supported liquid extraction.
Part No: AN867Issued year: 2016File size: 1.28mbFile type: pdf
This application note describes the extraction, using ISOLUTE SLE+ supported liquid extraction columns, of THC, THCA and Carboxy-THC from oral fluid matrix collected using the NeoSal device, prior to GC/MS analysis.
Part No: AN840Issued year: 2015File size: 0.7mbFile type: pdf
This application note describes the extraction of Δ9-THC, 11-hydroxy- Δ9-THC and 11-nor-9-carboxy-THC from whole blood matrix, prior to GC/MS analysis.
An extremely simple sample pre-treatment and extraction method is employed to extract the parent THC and main metabolites from complex whole blood matrix, delivering high, reproducible analyte recoveries.
Part No: AN819Issued year: 2014File size: 1.54mbFile type: pdf
This application note describes the extraction of THC, THCA and Carboxy-THC from oral fluid matrix collected using the Intercept Oral Fluid Drug Test Kit (Orasure Technologies), prior to GC/MS analysis.
Part No: PPS587Issued year: 2019File size: 3.58mbFile type: pdf
Recreational marijuana use is currently legal in eleven U.S. states. Fourteen additional states have decriminalized marijuana, but have not legalized it. It is increasingly important for laboratories to develop accurate testing methods for THC, and its related compounds, in biological matrices of those that may be driving or committing crimes under the influence of marijuana. Biotage provides simple and effective sample prep protocols for cleanup of blood, urine, and oral fluid samples that produce reliable results, and are automation compatible.
Part No: P153.v.1Issued year: 2017File size: 1.12mbFile type: pdf
This poster describes a simple but effective method for screening a range of pesticides and drugs of abuse from liver samples. Liver tissue is homogenized using the Biotage® Lysera, and the subsequent extract is cleaned up using ISOLUTE SLE+ supported liquid extraction columns.
Part No: P198.V.3Issued year: 2020File size: 0.59mbFile type: pdf
Hair analysis is growing in popularity due to the non-invasive nature
of the sample collection. Although not used as routinely as other
matrices such as blood or urine it does have advantages in that the
matrix can indicate prolonged drug exposure. This can provide
valuable information with respect to therapeutic drug regimens or
in abused drug abstinence cases. MSACL, 2020.