The method described in this application note achieves high recoveries of THC and an extended suite of common metabolites in oral fluid from Quantisal (Immunalysis) oral fluid collection devices. This application note describes effective and efficient ISOLUTE SLE+ protocols optimized for sample loading volumes of either 300 μL or 800 μL. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 64% with RSDs of <10% for all analytes.

11-hydroxy- Δ9-tetrahydrocannabinol (THC-OH) 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH) 11-nor-9-carboxy- Δ9-tetrahydrocannabivarin (THC-V-COOH) 11-nor-9-carboxy- Δ9tetrahydrocannabinol glucuronide (THC-COOH-g 11-nor-9-carboxy-Δ9-THC glucuronide Application Notes Cannabidiol Cannabigerol Column Drugs of Abuse English Forensic LC-MS/MS Oral fluid Saliva THC THC metabolites THC-COOH Δ9-tetrahydrocannabinol (THC) Δ9-tetrahydrocannabivarin (THCV)

This poster demonstrates protocols for the determination of a range of drugs of abuse following collection with the NeoSal™ oral fluid device and GC/MS analysis. The drug suites includes amphetamines and synthetic cathinones, barbiturates, benzodiazepines, cocaine, opiates, cannabinoids and synthetic cannabinoids. SOFT 2017, Boca Raton

Amphetamines Barbiturates Benzodiazepines Cannabinoid Cathinones Clinical Cocaines Column English Forensic GC-MS Opiates Oral fluid Posters Saliva Synthetic Cannabinoids (SPICE)

This poster demonstrates two fast and robust methods for the extraction of buprenorphine and norbuprenorphine from urine, oral fluid and whole blood. SOFT 2015

96-well plate Buprenorphine Clinical Column English Forensic LC-MS/MS Norbuprenorphine Oral fluid Pharmaceuticals Posters Saliva Urine Whole blood

Buprenorphine and Norbuprenorphine are typically problematic for analysis due to analyte stability issues during sample preparation. This poster will demonstrate two fast and robust methods for the extraction of buprenorphine and norbuprenorphine in urine (using EVOLUTE EXPRESS CX), oral fluid (using EVOLUTE EXPRESS CX) and whole blood (using ISOLUTE SLE+). MSACL 2016

96-well plate Buprenorphine Clinical Column English Forensic LC-MS/MS Norbuprenorphine Oral fluid Pain Management Posters Saliva Synthetic urine Urine Whole blood

This poster demonstrates the extraction of a range of drugs of abuse from oral fluid, collected with common collection devices, prior to UPLC-MS/MS analysis. The target analyte list includes benzodiazepines, z drugs, amphetamines, cathinones, opiates, cocaine, buprenorphine, PCP, THC-COOH, fentanyl and ketamine. SOFT 2014

11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH) 2-OH-Et-Flurazepam 6-acetylmorphine (MAM) 7-Aminoclonazepam 7-Aminoflunitrazepam Alprazolam Amphetamine Amphetamines Benzodiazepines Benzoylecgonine Bromazepam Buprenorphine Cathinones Clonazepam Cocaine Codeine Column Diazepam Dihydrocodeine (DHC) EDDP English Estazolam Fentanyl Flunitrazepam Flurazepam Forensic Hydrocodone Hydromorphone Ketamine LC-MS/MS Lorazepam MDA MDEA MDMA Mephedrone Methadone Methamphetamine Midazolam Morphine Nitrazepam Norbuprenorphine Nordiazepam Norfentanyl Norketamine Opiates Opiates Oral fluid Oxazepam Oxycodone Oxymorphone Phencyclidine (PCP) Posters Saliva Temazepam Triazolam Zaleplone Zolpidem Zopiclone z-drugs α-OH Triazolam α-OH alprazolam

This methodology has been designed to give an effective and efficient supported liquid extraction protocol for the clean-up and concentration of salivary cortisol levels. Cortisol is a steroid hormone that when measured from saliva can be used as an indication of stress. Analyte recoveries achieved using this method ranged from 96-99% with RSDs below 3% (n=7) for all analytes. Cortisol, Saliva, SLE, Supported Liquid Extraction, ISOLUTE, Clinical, Steroid Hormones, Stress

Application Notes Clinical Cortisol LC-MS/MS Saliva Steroid Hormones

This application note describes the extraction of 47 drugs of abuse from oral fluid matrix after sampling via Quantisal collection devices prior to analysis by UPLC-MS/MS. Optimised protocols for 200 uL and 500 uL sample volumes are included.

2-OH-Et-Flurazepam 6-acetylmorphine (MAM) 7-Aminoclonazepam 7-Aminoflunitrazepam Amphetamine Amphetamines Application Notes Benzodiazepines Benzoylecgonine Bromazepam Buprenorphine Cathinones Clinical Clonazepam Cocaine Codeine Column Diazepam Dihydrocodeine (DHC) Drugs of Abuse EDDP English Estazolam Fentanyl Flunitrazepam Flurazepam Forensic Hydrocodone Hydromorphone Ketamine LC-MS/MS Lorazepam MDA MDEA MDMA Mephedrone Methadone Methamphetamine Midazolam Morphine Nitrazepam Norbuprenorphine Nordiazepam Norfentanyl Opiates Oral fluid Oxazepam Oxycodone Oxymorphone Pain Management Phencyclidine Phencyclidine (PCP) Saliva Temazepam Triazolam Zaleplone Zolpidem Zopiclone α-OH Triazolam α-OH alprazolam

This application note describes the extraction of 47 drugs of abuse and metabolites from oral fluid matrix collected using the Intercept Oral Fluid Drug Test Kit (Orasure Technologies), prior to UPLC-MS/MS analysis. The sample preparation is optimised to minimise matrix effects due to the buffers used in the collection device. Estimated LOQs range from 0.1-1 ng/mL for the various analytes.

2-Hydroxyethylflurazepam 2-OH-Et-Flurazepam 6-acetylmorphine (MAM) 7-Aminoclonazepam 7-Aminoflunitrazepam Alprazolam Amphetamine Amphetamines Application Notes Basic drugs Benzodiazepines Benzoylecgonine Bromazepam Buprenorphine Clinical Clonazepam Cocaine Cocaines Codeine Column Diazepam Dihydrocodeine (DHC) Drugs of Abuse EDDP English Estazolam Fentanyl Flunitrazepam Flurazepam Forensic Hydrocodone Hydromorphone Ketamine LC-MS/MS Lorazepam MDA MDEA MDMA Mephedrone Methadone Methamphetamine Midazolam Morphine Nitrazepam Norbuprenorphine Nordiazepam Norfentanyl Norketamine Opiates Oral fluid Oxazepam Oxycodone Oxymorphone Pharmaceuticals Phencyclidine (PCP) Saliva Temazepam Triazolam Zaleplone Zolpidem Zopiclone α-OH Triazolam α-OH alprazolam

This application note describes the extraction of 47 drugs of abuse and metabolites from oral fluid matrix after sampling via Oral-Eze collection devices, prior to UPLC-MS/MS analysis using ISOLUTE SLE+ supported liquid extraction columns. Estimated LOQs of between 0.005 and 0.75 ng/mL (analyte dependant) are achieved,.

2-OH-Et-Flurazepam 6-acetylmorphine (MAM) 7-Aminoclonazepam 7-Aminoflunitrazepam Alprazolam Amphetamine Amphetamines Application Notes Basic Benzodiazepines Benzoylecgonine Bromazepam Buprenorphine Clinical Clonazepam Cocaine Cocaines Codeine Column Diazepam Dihydrocodeine (DHC) Drugs of Abuse EDDP English Estazolam Fentanyl Flunitrazepam Flurazepam Forensic Hydrocodone Hydromorphone Ketamine LC-MS/MS Lorazepam MDA MDEA MDMA Mephedrone Methadone Methamphetamine Midazolam Morphine Nitrazepam Norbuprenorphine Nordiazepam Norfentanyl Norketamine Opiates Oral fluid Over the counter pharmaceuticals Oxazepam Oxycodone Oxymorphone Pain Management Pharmaceuticals Phencyclidine (PCP) Saliva Temazepam Triazolam Zaleplone Zolpidem Zopiclone α-OH Triazolam α-OH alprazolam

This application note describes the extraction, using ISOLUTE SLE+ supported liquid extraction columns, of amphetamine style compounds from oral fluid matrix collected using the NeoSalTM device, prior to GC/MS analysis.

2C-B 5-APB 6-APB Amphetamine Amphetamines Application Notes BZP Butylone Column Designer stimulants Drugs of Abuse English Ethylone Forensic MDA MDEA MDMA MDPV Mephedrone Methamphetamine Methcathinone Methedrone Methylone Naphyrone Oral fluid Saliva TFMPP mCPP pMMA

Antiepileptic drugs are prescribed to suppress seizures in epilepsy patients. A variety of different types of AEDs have been synthesized to pharmacologically address different types of epilepsy. The ability to therapeutically monitor these drugs in patients is necessary for maintaining optimal medical care and managing any adverse effects of thedrug. A fast and clean extraction method is needed that works in a variety of biological matrices and affords a high throughput workflow. In this poster, ISOLUTE SLE+ is demonstrated as an effective way to extract AEDs from serum, oral fluid and urine with good efficiency and recovery. The method was developed on a 96 well plate format to facilitate a high throughput workflow model. MSACL 2014

96-well plate Antiepileptics Carbamazepine 10,11 epoxide Clinical English Felbamate Gabapentin LC-MS/MS Oral fluid Oxcarbazepine Posters Rufinamide Saliva Serum Tigabatine Urine Vigabatrin

In this poster, ISOLUTE SLE+ is demonstrated as an effective way to extract antiepileptic drugs from serum, oral fluid and urine with good efficiency and recovery. The method was developed on a 96-well plate format to facilitate a high throughput workflow model. ASMS 2014

96-well plate Antiepileptics Carbamazepine 10,11 epoxide Clinical English Felbamate Gabapentin LC-MS/MS Oral fluid Oxcarbazepine Posters Rufinamide Saliva Serum Tigabatine Urine Vigabatrin

This application note describes the extraction of barbiturates from oral fluid matrix collected using the Intercept Oral Fluid Drug Test Kit(Orasure Technologies), prior to GC/MS analysis. The ISOLUTE SLE+ protocol is optimized for 400 μL and 1 mL sample capacity formats. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 90% with RSDs lower than 7% for all analytes.

Amobarbital Application Notes Barbiturates Butabarbital Butalbarbital Column Drugs of Abuse Forensic Hexobarbital LC-MS/MS Oral fluid Pentobarbital Phenobarbital Saliva Secobarbital

This application note describes the extraction of barbiturates, using ISOLUTE SLE+ supported liquid extraction columns, from oral fluid matrix collected using the NeoSal collection device, prior to GC/MS analysis.

Amobarbital Application Notes Barbiturates Butabarbital Butalbarbital Column Drugs of Abuse English Forensic GC-MS Hexobarbital Oral fluid Pentobarbital Phenobarbital Saliva Secobarbital

This application note describes the extraction of Butalbarbital, Butabarbital, Amobarbital, Pentobarbital,Secobarbital, Hexobarbital and Phenobarbital from oral fluid matrix collected using the Oral-Eze® collection devices, prior to GC/MS analysis.

Amobarbital Application Notes Barbiturates Butabarbital Butalbarbital Column English Forensic GC-MS Hexobarbital Oral fluid Pentobarbital Phenobarbital Saliva Secobarbital

This application note describes the extraction of barbiturates from oral fluid matrix collected using Quantisal™ collection devices (Immunalysis), prior to GC/MS analysis. The ISOLUTE SLE+ protocol is optimized for 400 μL and 1 mL sample capacity formats. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 88% with RSDs lower than 6% for all analytes.

96-well plate Amobarbital Application Notes Butabarbital Butalbarbital Forensic GC-MS Hexobarbital Oral fluid Pentobarbital Phenobarbital Saliva Secobarbital

This application note describes the extraction, using ISOLUTE SLE+ supported liquid extraction columns, of benzodiazepine compounds from oral fluid matrix collected using the NeoSalTM devices, prior to GC/MS analysis.

2-OH-Et-Flurazepam 7-Aminoflunitrazepam Alprazolam Application Notes Benzodiazepines Bromazepam Clonazepam Column Diazepam Drugs of Abuse English Flurazepam Forensic LC-MS/MS Lorazepam Midazolam Nitrazepam Nordiazepam Oral fluid Oxazepam Saliva Temazepam Triazolam α-OH Triazolam α-OH alprazolam

This application note describes a simple and robust mixed mode cation exchange solid phase extraction protocol using EVOLUTE® EXPRESS CX 96 well plates for the extraction of buprenorphine and norbuprenorphine from oral fluid. Excellent extract cleanliness allows quantitation of the analytes across the range 0.1 ng/mL to 100 ng/mL using LC-MS/MS.

96-well plate Application Notes Buprenorphine Clinical English Forensic LC-MS/MS Norbuprenorphine Oral fluid Pain Management Saliva

This application note describes the extraction of cocaine and selected metabolites from oral fluid matrix following the collection with the NeoSalTM device, prior to GC/MS analysis.

Anhydroecgonine Methyl Ester AEME Anhydroecgonine methyl ester Application Notes Benzoylecgonine Cocaethylene Cocaine Cocaines Column Drugs of Abuse Ecgonine Methyl Ester EME Ecgonine methyl ester English Forensic GC-MS Norcocaine Oral fluid Saliva

This application note describes the extraction of a suite of NBOMe designer drugs from oral fluid matrix with analysis by laser diode thermal desorption (LDTD)-MS/MS.

25B-NBOMe 25C-NBOMe 25D-NBOMe 25E-NBOMe 25H-NBOMe 25N-NBOMe 25T2-NBOMe Application Notes Column Drugs of Abuse English Forensic LDTD-MS/MS Legal highs NBOMes Oral fluid Saliva