This application note demonstrates the use of automated solid phase extraction for a variety of pharmaceutical compounds in drinking water, wastewater and surface water. The criteria specified in US EPA Method 1694 was met, even at very low concentrations. For lower concentrations a larger sample of water may be processed.

4790 Acetaminophen Albuterol Albuterol Application Notes Atenolol Caffeine Carbamazepine Cotinine DEET Dehydronifedipine Diclofenac Diltiazem Diphenhydramine hydrochloride Disk Drinking water English Environmental Gemfibrozil Ground water Ibuprofen LC-MS TOF Lamotrigine Metoprolol Naproxen Pharmaceuticals Sulfadimethoxine Sulfamethoxazole Triclocarban Trimethoprim Waste water

Pesticides have gone far in increasing food supply, important in a world expecting to have 9.8 billion people by 2050. However, the mechanisms that make pesticides effective in eliminating pests can also cause harm to humans and animals. News stories continue to point to contamination through spills and exposure through the respiratory system.

5000 Application Notes Disk EPA 608.3 Analytes English Environmental GC-MS US EPA Method 608.3 Waste water

Polycyclic aromatic hydrocarbons (PAHs) can be found in a number of water types, such as drinking water, surface water, ground water and wastewater. There are serval types of extraction techniques that can be implemented to remove them from water for measurement including liquid-liquid extraction (LLE), continuous liquid-liquid extraction (CLLE) and solid phase extraction (SPE). With LLE and CLLE there is little selectivity within the methods for difficult matrices or other compound interferences.

5000 Application Notes Disk Drinking water English Environmental GC-MS Ground water PAHs Polyaromatic Hydrocarbons (PAH) Waste water

The Biotage® VacMaster™ Disk is a unit for performing manual, disk-based extractions designed for high productivity laboratories. This unit is an excellent first step for labs interested in moving from liquid-liquid extraction (LLE) to solid phase extraction (SPE) for sample preparation in the environmental, food and beverage, and agriculture industries.

Disk English Environmental Hexane Extractable Material Petroleum Product Notes US EPA Method 1664 Waste water

EPA Method 1657 describes the procedure to determine low ppb levels of organophosphorous pesticides in wastewater. The disks compared in this study are: “Certified for Automation” Atlantic™ C18 (47 mm) and 3M Empore™ High Performance Extraction Disk C18 (47 mm).

4790 Application Notes Azinphos methyl Chlorpyrifos Demeton (O&S) Diazinon Disk Disulfoton EPA 1657 Analytes EPN English Environmental Ethion Ethyl parathion GC-FPD Malathion Methyl parathion Organophosphate Pesticide Waste water

EPA Method 608 describes the procedure to determine low parts per billion levels of organochlorine pesticides, polychlorinated biphenyls, toxaphene and chlordane in wastewater. The laboratory that collected the data presented in this application note uses EPA method 608 ATP3M0222 as the approved SPE technique for waste water extraction. The disks compared in this study are : “Certified for Automation” Atlantic™ C18 (47 mm) and 3M Empore™ High Performance Extraction Disk C18 (47 mm). Comparisons were made using side-by-side extraction and identical calibration parameters.

4,4,DDD 4790 Aldrin Application Notes DDE Dieldrin EPA 608 Analytes Endosulfan Endosulfan sulfate Endrin Endrin Aldehyde Endrin Ketone English Environmental GC/ECD Heptachlor Heptachlor epoxide Methoxychlor Organochlorine pesticides (OCP) PCB PCB1016 PCB1260 PCBs TCMX Waste water α-BHC β-BHC γ-BHC δ-BHC

The purpose of this application is to demonstrate the viability of a solid phase extraction (SPE) method utilizing the Horizon Technology SPE-DEX 1000/3000XL Controller with application firmware version 2.2 to extract samples for EPA Method 1664A/B and fulfill all QC requirements.

Application Notes Disk EPA 1664 A Analytes EPA 1664 B Analytes English Environmental Gravimetric Oil & Grease US EPA Method 1664 Waste water

The City of Fort Worth, Water Department conducted an evaluation of the Atlantic™ solid phase extraction (SPE) C18 disks using EPA Method 608. This application note describes the procedure to determine low ppb levels of organochlorine pesticides and PCBs in wastewater. This procedure used methylene chloride as the primary extraction solvent, followed by a hexane solvent exchange. Extracts were then analyzed by GC/ECD using a pressure pulse injection technique.

4,4,DDD 4790 Aldrin Application Notes DDE Disk EPA 608 Analytes Endosulfan Endosulfan sulfate Endrin Endrin Aldehyde Endrin Ketone English Environmental GC/ECD Heptachlor Heptachlor epoxide Methoxychlor Organochlorine pesticides (OCP) Organochlorines PCB PCB1016 PCB1260 PCBs TCMX US EPA Method 608.3 Waste water α-BHC β-BHC γ-BHC δ-BHC

Method 1657 describes the procedure to determine low ppb levels of organophosphorus pesticides in municipal and industrial wastewater. The City of Fort Worth Water Department implemented Automated SPE for the analysis of organophosphorous pesticides by EPA Method 1657, using the Atlantic™ C18 solid phase extraction disk. The Biotage® Horizon 4790 Automated Extraction System with Envision® Platform controller, and the DryVap® Automated Drying and Concentration System were used in this application note.

4790 Application Notes Azinphos methyl Chlorpyrifos Demeton (O&S) Diazinon Disk Disulfoton EPA 1657 Analytes EPN English Environmental Ethion Ethyl parathion GC-FPD Malathion Methyl parathion Organophosphate Pesticide Waste water

This application note demonstrates recoveries of all the analytes within tables 1–2 of EPA method 625.1 (plus a few analytes from table 3) obtained using the Biotage® Horizon 5000 with Atlantic® 8270 One Pass solid phase extraction disks, 8270 Carbon Cartridges Max-Detect cartridges, and 1.0 Micron Atlantic® Fast Flow Pre-Filters for analyte extraction. The DryDisk® Solvent Drying System and the TurboVap® II are used for solvent drying and concentration and analysis is by GC-MS.

Application Notes Disk English Environmental GC-MS SVOC Semi-Volatile Organics US EPA Method 625 Waste water

This application note describes the use of the Biotage® VacMaster™ Disk to extract n-hexane-extractable materials (HEM) from wastewaters per EPA Method 1664B. The procedure outlined in this document was used to generate a minimum level of quantitation (ML) and an initial demonstration of capability (IDC). Data for those studies is presented here.

Application Notes Disk EPA 1664 A Analytes EPA 1664 B Analytes English Environmental Hexane Extractable Material Oil & Grease Petroleum US EPA Method 1664 Waste water

The US EPA monitors a variety of chemicals in water that may cause harm to humans or wildlife in order to minimize exposure. Method 625 was developed by the Office of Science and Technology in the Clean Water program to monitor a large suite of semivolatile chemicals in wastewater for compliance with the National Pollution Discharge Elimination System (NPDES). NPDES is a system of permitting that regulates the characteristics of water that is released into a waterway, defined by industrial category. The permitting levels are set depending on the waterway’s use.

5000 Application Notes Disk EPA 625.1 Analytes English Environmental GC-MS US EPA Method 625 Waste water

n-Hexane-extractable material (HEM), often termed oil & grease, is an operationally-defined general measurement used around the world to help assess water pollution due to a variety of hydrocarbons, including dissolved aromatics, benzene, toluene, xylene and dispersed polynuclear aromatic hydrocarbons (PAHs), aliphatics, naphthenic and fatty acids.1

Application Notes Disk English Environmental Hexane Extractable Material Oil & Grease Oil & Grease US EPA Method 1664 Waste water

US EPA method 6251 is used to determine acidic, basic, and neutral semi-volatile organic compounds (SVOC) in municipal and industrial wastewater using GC/MS, in many cases for National Pollution Discharge Elimination System (NPDES) compliance. In method 625 revision A, the method is applied to a total possible list of 364 compounds that include; polynuclear aromatic hydrocarbons, chlorinated hydrocarbons, pesticides, phthalate esters, organophosphate esters, nitrosamines, haloethers, aldehydes, ethers, ketones, anilines, pyridines, quinolones, aromatic nitro compounds, and phenols.

4790 Application Notes Disk EPA 625.1 Analytes Environmental US EPA Method 625 Waste water

Environmental methods for water analysis often specify sampling 1 L of water to provide the sensitivity needed for determination of contaminants at low concentrations for health monitoring. In the past decade sensitivity of the analytical technique (GC/MS) has improved and with split/splitless introduction and large volume injection options a wide range of concentrations can be effectively measured.

4790 Application Notes Disk EPA 625.1 Analytes English Environmental GC-MS Waste water

The US EPA monitors a variety of chemicals in water that may cause harm to humans or wildlife to minimize exposure. Method 625 was developed by the Office of Science and Technology in the Clean Water program to monitor a large suite of semivolatile chemicals in wastewater for compliance with the National Pollution Discharge Elimination System (NPDES).

4790 Application Notes EPA 625.1 Analytes English Environmental GC-MS US EPA Method 625 Waste water