The potential harm caused by synthetic cannabinoids is a significant concern since exposures and reports of human fatalities due to these substances is widespread [5]. Detection of these relatively new type designer drugs in blood is essential in forensic toxicology, other scientific and medical fields. Cannabinoids, SLE, SLE+, Supported Liquid Extraction,

CP47,497 CP47,497-C8 Column Forensic HU210 JWH-018 JWH-073 JWH-200 JWH-250 JWH019 LC-MS/MS Posters Synthetic Cannabinoids (SPICE) Whole blood

This is the importable .exp method for the application note AN876

PEth Whole blood

This poster discusses the potential for a single extraction protocol for various drugs of abuse classes in whole blood prior to UPLC-MS/MS analysis. SOFT 2017, Boca Raton also presented at SFTA, Marseille, France, 2018

Amphetamines Benzodiazepines Cannabinoid Cathinones Clinical Cocaines Column Drugs of Abuse English Extrahera Forensic LC-MS/MS Opiates Posters THC metabolites Whole blood z-drugs

This poster demonstrates two fast and robust methods for the extraction of buprenorphine and norbuprenorphine from urine, oral fluid and whole blood. SOFT 2015

96-well plate Buprenorphine Clinical Column English Forensic LC-MS/MS Norbuprenorphine Oral fluid Pharmaceuticals Posters Saliva Urine Whole blood

Buprenorphine and Norbuprenorphine are typically problematic for analysis due to analyte stability issues during sample preparation. This poster will demonstrate two fast and robust methods for the extraction of buprenorphine and norbuprenorphine in urine (using EVOLUTE EXPRESS CX), oral fluid (using EVOLUTE EXPRESS CX) and whole blood (using ISOLUTE SLE+). MSACL 2016

96-well plate Buprenorphine Clinical Column English Forensic LC-MS/MS Norbuprenorphine Oral fluid Pain Management Posters Saliva Synthetic urine Urine Whole blood

Endogenous phospholipids present in biological fluids are a major problem in LCMS/ MS analysis as they are often very difficult to remove during sample preparation. When phospholipids are not removed, they retain very strongly on reversed phase analytical columns. If high organic (end of run) washes are not incorporated into the LC methods these matrix components may elute in subsequent analyses causing regions of suppression/enhancement leading to inaccurate quantitation. This poster evaluates the use of polymer-based solid phase extraction (SPE) sorbents, incorporating hydrophobic and various mixedmode retention mechanisms to address the problems associated with phospholipid removal. Phospholipid, EVOLUTE, STRATA X, OASIS, WATERS, AX, WAX, CX, WCX, ABN, ASMS 2011

Application Notes Aqueous Biological fluids Column LC-MS/MS Plasma RapidTrace Serum Urine Whole blood

This poster demonstrates a fast, reliable protocol to extract multiple drug of abuse panels from whole blood using a common supported liquid extraction methodology. This benefits laboratory workflow where multiple assays are run each day, saving both worker hours and consumable costs. SOFT 2015 ASMS 2016 TIAFT 2016

2-Hydroxyethylflurazepam 2C-B 5-APB 6-APB 6-acetylmorphine (MAM) 7-Aminoclonazepam 7-Aminoflunitrazepam Alprazolam Amobarbital Amphetamine Anhydroecgonine Methyl Ester AEME BZP Benzoylecgonine Bromazepam Butabarbital Butalbarbital Butylone Clinical Clonazepam Cocaethylene Cocaine Cocaines Codeine Column Diazepam Dihydrocodeine (DHC) Drugs of Abuse Ecgonine Methyl Ester EME English Estazolam Ethylone Flunitrazepam Flurazepam Forensic GC-MS Hexobarbital Hydrocodone Hydromorphone Lorazepam MDA MDEA MDMA MDPV Mephedrone Methamphetamine Methcathinone Methedrone Methylone Midazolam Morphine Naphyrone Nitrazepam Nordiazepam Oxazepam Oxycodone Oxymorphone Pentobarbital Phenobarbital Posters Secobarbital TFMPP Temazepam Triazolam Whole blood mCPP pMMA α-OH Triazolam α-OH alprazolam

This poster compares the performance of manual processing to a novel automated sample preparation system prior to GC/MS or LC-MS/MS analysis in forensic toxicology applications. Emphasis is placed on the potential for 96-well cross contamination and strategies for its elimination. TIAFT 2016 SOFT 2016

2-Hydroxyethylflurazepam 2-OH-Et-Flurazepam 6-acetylmorphine (MAM) 7-Aminoclonazepam 7-Aminoflunitrazepam 96-well plate Alprazolam Amphetamine Amphetamines Benzodiazepines Bromazepam Buprenorphine Clinical Clonazepam Codeine Column Diazepam Dihydrocodeine (DHC) Drugs of Abuse EDDP English Estazolam Extrahera Fentanyl Flunitrazepam Flurazepam Forensic GC-MS Hydrocodone Hydromorphone Ketamine LC-MS/MS Lorazepam MDA MDEA MDMA Mephedrone Methadone Methamphetamine Midazolam Morphine Nitrazepam Norbuprenorphine Nordiazepam Norfentanyl Norketamine Opiates Opiates Oxazepam Oxycodone Oxymorphone Phencyclidine (PCP) Posters Temazepam Triazolam Urine Whole blood Zaleplone Zolpidem Zopiclone z-drugs α-OH Triazolam α-OH alprazolam

This poster describes a simple supported liquid extraction (SLE) method for extraction of THC and metabolites from whole blood and urine, suitable for GC-MS analysis. SOFT 2015

11-hydroxy- Δ9-tetrahydrocannabinol (THC-OH) 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH) Column Drugs of Abuse English Forensic GC-MS Posters THC THC-COOH Urine Whole blood Δ9-tetrahydrocannabinol (THC)

This application note describes the extraction, using ISOLUTE SLE+ supported liquid extraction columns, of 49 drugs of abuse from whole blood, prior to UPLC-MS/MS analysis. High, reproducible drug recoveries are achieved, with sub ng/mL LOQs for most analytes. The method is easily automated using Biotage® Extrahera.

11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH) 2-Hydroxyethylflurazepam 6-acetylmorphine (MAM) 7-Aminoclonazepam 7-Aminoflunitrazepam Alprazolam Amphetamine Benzodiazepines Benzoylecgonine Bromazepam Buprenorphine Clonazepam Cocaine Cocaines Codeine Column Diazepam Dihydrocodeine (DHC) Drugs of Abuse EDDP English Estazolam Extrahera Fentanyl Flunitrazepam Flurazepam Forensic Hydrocodone Hydromorphone Ketamine LC-MS/MS Lorazepam Lysergic acid diethylamide (LSD) MDA MDEA MDMA Mephedrone Methadone Methamphetamine Midazolam Morphine Nitrazepam Norbuprenorphine Nordiazepam Norfentanyl Norketamine Opiates Oxazepam Oxycodone Oxymorphone Pain Management Phencyclidine (PCP) THC metabolites THC-COOH Temazepam Triazolam Whole blood Zaleplone Zolpidem Zopiclone z-drugs α-OH Triazolam α-OH alprazolam

Immunosuppressants inhibit or prevent the activity of the immune system used to prevent rejection of transplanted organs and treat autoimmune diseases. Due to the risks of immunodeficiency, those under treatment must undergo constant therapeutic drug monitoring (TDM) requiring reliable and robust analytical techniques for quantification of these drugs. Current methodologies use immunoassay techniques to measure immunosuppressant levels in patients which are expensive, time consuming and susceptible to issues with cross reactivity. The sample prep method in this application note offers an alternative approach to immunosuppressant analysis with LC-MS/MS giving more reliable and robust recoveries with no cross-reactivity and cost saving opportunities. Analyte recoveries range from 60-97% with RSDs all below 10%. Immunosuppressant, Tacrolimus, Sacrolimus, Everolimus, Cyclosporin A, Clinical, SLE+, SLE, Supported Liquid Extraction, immune, Clinical, Whole Blood, immunoassay,

Application Notes Clinical Cyclosporin A Everolimus Immunosuppressant LC-MS/MS Sacrolimus Sirolimus Tacrolimus Whole blood

This application note describes the extraction of a range of amphetamine-type compounds from whole blood, prior to GC/MS analysis. A protocol that allows the simultaneous extraction of various other drugs of abuse classes: barbiturates, benzodiazepines, cocaine and opiates, is also evaluated. ISOLUTE® SLE+ columns with 1 mL sample capacity are used to extract whole blood samples following a straightforward sample dilution. No protein precipitation or other pre-treatment is required prior to sample loading. The sample preparation procedure delivers clean extracts, good recoveries and RSD values and LLOQs from 10 ng/mL (analyte dependant).

2C-B 5-APB 6-APB Amphetamine Amphetamines Application Notes BZP Butylone Clinical Column English Ethylone Forensic GC-MS MDA MDEA MDMA MDPV Mephedrone Methamphetamine Methcathinone Methedrone Methylone Naphyrone TFMPP Whole blood mCPP pMMA

This procedure is recommended for the extraction of extensive panel of significant therapeutic and illicit drugs of abuse from whole blood and urine using mixed-mode cation exchange SPE columns. ISOLUTE HCX is a silica based mixed-mode sorbent that combines non-polar and strong cation exchange retention mechanisms for extraction of basic drugs from biological fluids. Ion exchange, Drugs of Abuse, DOA, Clinical,

2-Hydroxyethylflurazepam 4-Hydroxynordiazepam 7-Aminoclonazepam 7-Aminoflunitrazepam 7-Aminonitrazepam Alprazolam Application Notes Benzodiazepines Bromazepam Chlordiazepoxide Clinical Clonazepam Clozapine Demethylflunitrazepam Desalkylflurazepam Diazepam English Estazolam Flunitrazepam Flurazepam Forensic Hydroxyalprazolam Hydroxymidazolam Hydroxytriazolam LC-MS/MS Midazolam Nitrazepam Nordiazepam Oxazepam Prazepam Temazepam Triazolam Urine Whole blood Zolpidem

This application note describes the extraction of barbiturate compounds from whole blood, prior to GC/MS analysis. This protocol also allows the simultaneous extraction of various other drugs of abuse classes: amphetamines, benzodiazepines, cocaine and opiates. ISOLUTE® SLE+ columns with 1 mL sample capacity are used to extract whole blood samples following a straightforward sample dilution. No protein precipitation or other pre-treatment is required prior to sample loading. The sample preparation procedure delivers clean extracts, good recoveries and RSD values and LLOQs from 20 ng/mL (analyte dependant).

Amobarbital Application Notes Barbiturates Butabarbital Butalbarbital Clinical Column English GC-MS Hexobarbital Pentobarbital Phenobarbital Secobarbital Whole blood

This application note describes the extraction of benzodiazepine compounds from whole blood, prior to GC/MS analysis. This protocol also allows the simultaneous extraction of various other drugs of abuse classes: amphetamines, barbiturates, cocaine and opiates. ISOLUTE® SLE+ columns with 1 mL sample capacity are used to extract whole blood samples following a straightforward sample dilution. No protein precipitation or other pre-treatment is required prior to sample loading. The sample preparation procedure delivers clean extracts, good recoveries and RSD values and LLOQs from 10 ng/mL (analyte dependant).

2-OH-Et-Flurazepam 7-Aminoclonazepam 7-Aminoflunitrazepam Alprazolam Application Notes Benzodiazepines Bromazepam Clinical Clonazepam Column Diazepam English Estazolam Flunitrazepam Flurazepam Forensic GC-MS Lorazepam Midazolam Nitrazepam Nordiazepam Oxazepam Temazepam Triazolam Whole blood α-OH Triazolam α-OH alprazolam

This application note describes the extraction of buprenorphine and norbuprenorphine from whole blood using ISOLUTE® SLE+ Supported Liquid Extraction prior to LC-MS/MS analysis.

96-well plate Application Notes Buprenorphine Clinical English Forensic LC-MS/MS Norbuprenorphine Pain Management Whole blood

This application note describes the extraction of cocaine and major metabolites from whole blood, prior to GC/MS analysis. This protocol also allows the simultaneous extraction of various other drugs of abuse classes: amphetamines, barbiturates, benzodiazepines and opiates. ISOLUTE® SLE+ columns with 1 mL sample capacity are used to extract whole blood samples following a straightforward sample dilution. No protein precipitation or other pre-treatment is required prior to sample loading. The sample preparation procedure delivers clean extracts, good recoveries and RSD values and LLOQs from 20 ng/mL (analyte dependant).

Anhydroecgonine Methyl Ester AEME Anhydroecgonine methyl ester Application Notes Benzoylecgonine Clinical Cocaethylene Cocaine Cocaines Column Drugs of Abuse Ecgonine Methyl Ester EME English Forensic GC-MS Whole blood

This application note describes the extraction of cocaine, benzoylecgonine and cocaethylene from whole blood and human tissue using ISOLUTE HCX columns.

Application Notes Benzoylecgonine Butylone Cocaines Column Forensic LC-MS/MS Post-Mortem tissue Whole blood

This method was developed for the extraction of lysergic acid diethylamide (LSD) from whole blood using a mixed non-polar and cation exchange retention mechanism. LSD, BIOTAGE, ISOLUTE, HCX, DOA, Hallucinogen

Application Notes Column Forensic HPLC Hydromorphone Whole blood

This method demonstrates the extraction of Mephedrone (MCAT) from a range of biological fluids including plasma, urine and whole blood. This extraction is achieved through cation exchange using EVOLUTE CX, a mixed-mode resin-based SPE sorbent. Analytes were quantified using LC-MS/MS and recoveries were all above 90%. SPE, ION EXCHANGE, EVOLUTE, CX, STRATA X, OASIS, DOA

Application Notes Biological fluids Forensic LC-MS/MS Mephedrone Plasma Urine Whole blood