Part No: PPS347Issued year: 2014File size: 0.49mbFile type: pdf
Trends on chemical synthesis have changed over recent years, with a more targeted approach to molecular design becoming more prevalent. As a result, the speed with which a new compound can be synthesized is key to an efficient laboratory.
Part No: F0051403_01Issued year: 2014File size: 0.14mbFile type: pdf
The Speed-Vap IV is a modern evaporation system providing safe and simultaneous unattended evaporation of up to nine pans of evaporating solvent for extractable hydrocarbons such as hexane oil and grease extractions generated using methods such as US EPA 1664, ISO 11349:2010 or Standard Methods 5520. It can also be used to evaporate solvents from extracted soil hydrocarbons and fats from foods.
Part No: TN522Issued year: 2004File size: 0.03mbFile type: pdf
Microwave assisted organic synthesis has become an important tool to medicinal chemists for rapid organic synthesis. Thousands of research papers have appeared over the last decades on the application of microwave technology in organic synthesis.1 Some of the major advantages include a spectacular decrease in reaction time, improved conversions, clean product formation and wide scope for the development of new reaction conditions.
Part No: P215Issued year: 2020File size: 0.34mbFile type: pdf
Peptides are well suited for use as tool compounds to interrogate biological systems and processes due to their chameleonic ability to mimic a potential protein partner within the biological system of interest. Their use in this application continues to grow as the rules governing permeability across the plasma membrane are further elucidated. Unfortunately, a well-defined secondary structure is often required for productive interaction with the other protein partners within the system of interest. ACS, Spring, 2020
Part No: Issued year: 2002File size: 0.36mbFile type: pdf
The first decision that needs to be made is what the final analysis will be for the analyte. This will have an impact on the sample and cartridge size, as well as the final elution solvent. Gas chromatography offers higher sensitivity than HPLC, while HPLC is better suited for ionisable species and very high molecular weights. If LC-MS is available, minimal sample clean-up may be required.
Part No: PPS618Issued year: 2020File size: 1.91mbFile type: pdf
Load-Wash-Elute SPE, eliminating conditioning and equilibration steps can provide excellent extraction performance while reducing complexity and processing time. Elimination of the evaporation step using microelution SPE can also bring significant time savings.
Part No: P210Issued year: 2019File size: 0.56mbFile type: pdf
This poster evaluates two “pass through” solid phase extraction techniques: (protein removal/phospholipid depletion (ISOLUTE PLD+) and dual mode extraction (ISOLUTE HYDRO DME+)) and supported liquid extraction (ISOLUTE SLE+) for a group of about 100 drugs of abuse compounds in whole blood.
Part No: P167Issued year: 2017File size: 0.42mbFile type: pdf
For most organic and natural product chemists flash
chromatography is a necessary part of their research. As such, many chemists need quick isolation of at least one desired component from a crude mixture in relatively high yield and purity. This need for speed, purity, and yield pits these desires against each other as you can typically optimize on only two of the three goals.
In this poster, we will describe some techniques that help chemists optimize flash purification and maximize speed, yield, and purity.
Part No: P188Issued year: 2018File size: 0.33mbFile type: pdf
Herein we identified an optimized protocol
for removing an Acm protecting group using the Biotage®
Initiator+ Alstra™ and applied the protocol for a fully
automated synthesis with on-resin disulfide bond formation,
simplified with the Branches™ software feature. The results
presented herein provide a route amenable to the synthesis of
other disulfide rich peptides, greatly reducing the effort put
toward synthesizing these complex molecules.
Part No: P021Issued year: 2008File size: 1.87mbFile type: pdf
It is well known that traditional liquid-liquid extraction (LLE) provides very clean extracts prior to LC/MS analysis. Supported liquid extraction is analogous to traditional LLE, however, analyte partitioning takes place using an inert support material, rather than two immiscible liquids. This provides excellent extraction efficiencies while alleviating
many of the tedious liquid handling issues associated with LLE.
Part No: AN897Issued year: 2018File size: 2.27mbFile type: pdf
This application note describes a streamlined procedure for
sample pre-treatment and extraction of a panel of 49 drugs
of abuse from human hair, using Biotage® Lysera for matrix
micropulverization, prior to direct transfer to clean up using
ISOLUTE® SLE supported liquid extraction.
Part No: AN069-HORIssued year: 2011File size: 0.79mbFile type: pdf
Laboratories which take in a high volume of aqueous samples will know that every sample is different. Samples can contain varying amounts of suspended particulates and/or sediment due to either the source of the water, or the collection technique. Still other samples may form precipitates under elevated pH conditions resulting in
emulsions. These types of samples have historically proven very challenging when using solid phase extraction (SPE).
Part No: UI298.v1Issued year: 2019File size: 0.37mbFile type: pdf
1) Remember to add stir bar for efficient mixing. Catalysts, salts, or visible precipitate should be washed clear of head space and into solution. Particles adhering to glass head space could cause excessive heating increasing possibility of failure of vial. Stay within specified vial volume range (see diagram for proper filling).
Part No: TechTipIssued year: 2011File size: 0.04mbFile type: pdf
Within each disk holder there is a support screen. The disk holders that we will cover are used with a broad range of Horizon products. The placement and condition of the support screen is critical to the functioning of these products.
Part No: P080Issued year: 2014File size: 0.88mbFile type: pdf
This poster describes the benefits of supported liquid extraction and highlights its use in removal of endogenous matrix components that cause ion suppression/enhancement (matrix effects) in LC-MS/MS analyses.
Part No: P118Issued year: 2015File size: 0.48mbFile type: pdf
This poster examines the use of ISOLUTE SLE+ columns as an alternative to SPE for the extraction and clean up of hair samples containing drugs of abuse. SLE was found to be a simple, faster alternative to SPE for this type of analysis.