Part No: P077Issued year: 2014File size: 0.48mbFile type: pdf
This poster uses gel electrophoresis data to visually illustrate the amount of residual protein in extracts prepared using protein precipitation, supported liquid extraction and various silica and polymer based SPE approaches.
Part No: TN125.V.1Issued year: 2020File size: 0.8mbFile type: pdf
The ISOLUTE HCX series of mixed-mode SPE sorbents is based on a combination of strong cation exchange and non-polar chemistries. Basic drugs are retained by two primary retention mechanisms. This allows a rigorous interference elution regime to be used to elute interferences retained by either non-polar or cation exchange interactions alone. Only analytes with both non-polar and basic characteristics are extracted using the ISOLUTE HCX series of sorbents, providing an extremely pure final extract.
Part No: TN126Issued year: 2003File size: 0.06mbFile type: pdf
ISOLUTE non-polar SPE sorbents retain drugs from aqueous biological fluids through hydrophobic interactions. Endogenous compounds from the original sample matrix, such as proteins, are not retained. When the sorbent is rinsed, weakly retained compounds are eluted to waste, leaving strongly retained compounds to be eluted in the final elution solvent.
Part No: AN937Issued year: 2020File size: 0.88mbFile type: pdf
Axitinib is a type of molecularly targeted drug used in renal cell carcinoma. To avoid serious side effects and achieve maximal therapeutic efficacy, it is crucial to determine the correlation
between side effects on blood levels and treatment efficacy. Therapeutic Drug Monitoring (TDM) is one of the measures to achieve this.
In this application note, ISOLUTE® SLE+ was used as a sample preparation column for Axitinib determination in plasma using the principles of supported liquid extraction (SLE).
Part No: AN926Issued year: 2019File size: 0.93mbFile type: pdf
In this application note, ISOLUTE® SLE+ was used as a sample pretreatment column for mycophenolic acid determination in plasma using supported liquid extraction (SLE). UV based methods are commonly used to measure mycophenolic acid. In this application note, we have developed a method that can be used for both UV measurement and LC/ MS/MS measurement. LC/MS/MS assays are highly selective and less susceptible to the effects of other drugs used in combination during treatment.
Part No: AN928Issued year: 2019File size: 0.92mbFile type: pdf
Voriconazole (Figure 1) is a triazole-based antifungal drug that is widely used to treat fungal infections, including invasive aspergillosis, pulmonary aspergilloma, and candidemia. However, because
side effects such as prolonged QT syndromes, toxic epidermal necrolysis, and anaphylaxis are known, it is included in Therapeutic Drug Monitoring (TDM) regimes. This application note proposes a method that can be used for both UV and LC-MS/MS measurements.
Part No: AN898Issued year: 2018File size: 1.91mbFile type: pdf
This application note describes a procedure for sample
pre-treatment and extraction of a panel of 49 drugs of
abuse from human hair, using Biotage® Lysera for matrix
micropulverization, prior to clean up using ISOLUTE® SLE
supported liquid extraction. The application note contains
procedures optimized for 400 μL capacity column format and
200 μL and 400 μL supported liquid extraction plate formats,
for higher throughput applications. The methodology delivers
clean extracts and analyte recoveries mostly greater than
80% with RSDs lower than 10% for all analytes and LLOQ from