Part No: P077Issued year: 2014File size: 0.48mbFile type: pdf
This poster uses gel electrophoresis data to visually illustrate the amount of residual protein in extracts prepared using protein precipitation, supported liquid extraction and various silica and polymer based SPE approaches.
Part No: TN125Issued year: 2003File size: 0.07mbFile type: pdf
The ISOLUTE HCX series of mixed-mode SPE sorbents is based on a combination of strong cation exchange and non-polar chemistries. Basic drugs are retained by two primary retention mechanisms. This allows a rigorous interference elution regime to be used to elute interferences retained by either non-polar or cation exchange interactions alone. Only analytes with both non-polar and basic characteristics are extracted using the ISOLUTE HCX series of sorbents, providing an extremely pure final extract.
Part No: TN126Issued year: 2003File size: 0.06mbFile type: pdf
ISOLUTE non-polar SPE sorbents retain drugs from aqueous biological fluids through hydrophobic interactions. Endogenous compounds from the original sample matrix, such as proteins, are not retained. When the sorbent is rinsed, weakly retained compounds are eluted to waste, leaving strongly retained compounds to be eluted in the final elution solvent.
Part No: AN937Issued year: 2020File size: 0.88mbFile type: pdf
Axitinib is a type of molecularly targeted drug used in renal cell carcinoma. To avoid serious side effects and achieve maximal therapeutic efficacy, it is crucial to determine the correlation
between side effects on blood levels and treatment efficacy. Therapeutic Drug Monitoring (TDM) is one of the measures to achieve this.
In this application note, ISOLUTE® SLE+ was used as a sample preparation column for Axitinib determination in plasma using the principles of supported liquid extraction (SLE).
Part No: AN926Issued year: 2019File size: 0.93mbFile type: pdf
In this application note, ISOLUTE® SLE+ was used as a sample pretreatment column for mycophenolic acid determination in plasma using supported liquid extraction (SLE). UV based methods are commonly used to measure mycophenolic acid. In this application note, we have developed a method that can be used for both UV measurement and LC/ MS/MS measurement. LC/MS/MS assays are highly selective and less susceptible to the effects of other drugs used in combination during treatment.
Part No: AN928Issued year: 2019File size: 0.92mbFile type: pdf
Voriconazole (Figure 1) is a triazole-based antifungal drug that is widely used to treat fungal infections, including invasive aspergillosis, pulmonary aspergilloma, and candidemia. However, because
side effects such as prolonged QT syndromes, toxic epidermal necrolysis, and anaphylaxis are known, it is included in Therapeutic Drug Monitoring (TDM) regimes. This application note proposes a method that can be used for both UV and LC-MS/MS measurements.
Part No: AN898Issued year: 2018File size: 1.91mbFile type: pdf
This application note describes a procedure for sample
pre-treatment and extraction of a panel of 49 drugs of
abuse from human hair, using Biotage® Lysera for matrix
micropulverization, prior to clean up using ISOLUTE® SLE
supported liquid extraction. The application note contains
procedures optimized for 400 μL capacity column format and
200 μL and 400 μL supported liquid extraction plate formats,
for higher throughput applications. The methodology delivers
clean extracts and analyte recoveries mostly greater than
80% with RSDs lower than 10% for all analytes and LLOQ from
Part No: P165Issued year: 2017File size: 0.32mbFile type: pdf
This poster evaluates 3 different sample preparation approaches (ISOLUTE SLE+, EVOLUTE EXPRESS ABN, EVOLUTE EXPRESS CX) for extraction of large multi-drug urine panels.
Each approach is assessed in terms of suitability for extraction of analytes with different different properties (pka, LogP etc).
Part No: AN932Issued year: 2020File size: 2.13mbFile type: pdf
There are several different extraction methods that can be used to extract synthetic benzodiazepines from biological matrices such as urine and blood. This application note evaluates three common extraction techniques, detailing the methodology used, and comparing results in terms of analyte recovery and matrix effects.
Part No: Issued year: 2007File size: 0.48mbFile type: pdf
•Extends the range of acidic, basic and neutral compounds that can be extracted using a single generic method, increasing method development productivity•Has an optimized pore structure which reduces the extraction of endogenous matrix components, and leads to cleaner extracts that give fewer matrix effects in LC-MS/MS analysis•Has optimized physical and chemical characteristics that improve reliability of SPE procedures•Has no secondary interactions, allowing the use of pure organic elution solvent to give high recoveries of very low drug concentrations
Part No: TN127Issued year: 2003File size: 0.28mbFile type: pdf
ISOLUTE HAX mixed-mode SPE sorbent is based on a combination of strong anion exchange and non-polar chemistries. Acidic drugs are retained by two primary retention mechanisms. This allows a rigorous interference elution regime to be used to elute interferences retained by either non-polar or anion exchange interactions alone. Only analytes with both non-polar and acidic characteristics are extracted usign the ISOLUTE HAX sorbent, providing an extremely pure final extract.
Part No: P193Issued year: 2019File size: 0.77mbFile type: pdf
This poster explores the use of very low sample and elution volumes with 10 mg format EVOLUTE EXPRESS CX products for urine panel analysis.
An extraction protocol using a 10 mg mixed-mode cation exchange sorbent (EVOLUTE® EXPRESS CX) was developed and various sample sizes were assessed to determine the optimal sample volume for a 98-compound DOA panel. A method involving microelution off of the sorbent requiring no evaporation or reconstitution steps were also developed.
MSACL NA 2019
Part No: PPS558Issued year: 2019File size: 0.25mbFile type: pdf
Biotage® Selekt purification platform working in tandem with improved Biotage® Sfär columns has led to a leap forward in speed of purification and a downward trend in solvent consumption. Our team has crunched the numbers, and we’re more than happy to reveal the results.