Part No: P186Issued year: 2018File size: 0.2mbFile type: pdf
With the growing popularity and use of mass-directed flash
chromatography to purify specific compounds, it is important to understand that the sample’s compounds may not be detected as expected (e.g. M+H or M-H ions) because they may fragment or form adducts in the mass detector. This is true with both synthetic and natural product research.
Part No: PPS410Issued year: 2015File size: 0.7mbFile type: pdf
User Case: Professor Roger Strömberg, Karolinska Institutet. One of Professor Strömberg’s projects at Karolinska Institutet
involves the synthesis of artificial nucleases and PNAzymes. In
2013, his research group acquired a Biotage® Initiator+ Alstra™
peptide synthesizer for making PNAs and peptides.
Part No: PPS409Issued year: 2015File size: 1.61mbFile type: pdf
RIKEN is Japan’s largest research organization with 3,400
researchers from more than 50 countries. The chemists in RIKEN’s
Sugiyama Laboratory use EVOLUTE® EXPRESS solid-phase extraction
plates from Biotage in the analysis of clinical trial samples
for pharmacokinetic studies.
Part No: PPS432Issued year: 2016File size: 0.4mbFile type: pdf
Customer Case: Hitachi, Ltd. Healthcare Innovation Center. Hitachi’s R&D group has developed a new method for analyzing pharmacodynamics in cultured cells, in a study aimed at improving the pass rate in clinical trials. Mr. Ryosuke Takahashi tells the story.
Part No: Issued year: 2013File size: 0.43mbFile type: pdf
User report: ISOLUTE SLE+. Shin Nippon Biomedical Laboratories, Ltd. use ISOLUTE® SLE+ to prepare biological samples for pharmacokinetic testing as part of a drug discovery and development program. We had the chance to speak with Mr. Mitsuhiko Kawabata and Ms. Akiko Toda of the pharmacokinetics group.
Part No: T82Issued year: 2009File size: 0.16mbFile type: pdf
Oil & Grease standards come in pre-measured Teflon tubes of 10 mL each. Each tube contains 20 mg of n-Hexadecane and Stearic Acid for a total concentration of 40 mg. Care must be taken upon the introduction of the spiking solution to the sample bottle.
Part No: PPS406Issued year: 2015File size: 2.25mbFile type: pdf
Tokyo based PeptiDream Inc. is a company specializing in non-standard peptide therapeutics containing non-standard amino acids, and conducts research and development of new drug candidates. PeptiDream uses the peptide synthesizers Syro I and Biotage® Initiator+ Alstra™ for the synthesis of various kinds of peptides to support drug discovery and development.
Part No: AN049-HORIssued year: 2009File size: 0.74mbFile type: pdf
The DryVap® Concentrator System was intentionally designed to provide enhanced recoveries of semivolatile organic compounds, such as those found in EPA Method 8270. Its unique design of using an internal immersion heater vs. an external water bath, vacuum, and a sparge gas which is directed onto the surface of the solvent during the final stages of the evaporation process, allows the DryVap® System to achieve very good recoveries for a variety of tests.
Part No: AN042Issued year: 2003File size: 0.08mbFile type: pdf
Thin-layer chromatography (TLC) is a commonly used method development tool for flash purification. Product mixtures separated using TLC generally can be purified by flash chromatography using the identical solvent system. The method transfer accuracy of TLC to flash is based on the TLC silica and flash silica properties. When identical, the equation 1/Rf = CV holds, where Rf is the retention factor of a compound separated by TLC, and CV is the number of column volumes required to elute a compound. Cartridge loading capacity is based on the difference in CV (DCV) between two adjacent compounds.
Part No: Issued year: File size: 0.62mbFile type: pdf
The utilization of bound reagents for the solution phase synthesis has become an important tool in synthesis of biologically active molecules. The disadvantage of this technique is the relative slow rate of reaction. Microwave irradiation has been used to overcome this problem and increase rate of reaction of solid-assisted solution phase synthesis. This presentation covers developing efficient and robust strategies for the preparation of an array of cyclic tertiary amines using microwave irradiation in conjunction with bound reagents and scavengers such as MP-cyanoborohydride.
Part No: Issued year: 2015File size: 0.94mbFile type: pdf
The crossreactivity of molecularly imprinted polymers (MIPs) and its
practical implications are discussed. Screening of MIP libraries is presented as a
fasttrack route to discovery of resins selective towards new targets, exploiting the
fact that MIPs imprinted with one type of template molecule also show recognition
to related and sometimes also to apparently unrelated molecules. Several examples
from our own and others’ studies are presented that illustrate this crossreactivity and
the pattern of recognition is discussed for selected examples.
Part No: PPS482Issued year: 2018File size: 1.01mbFile type: pdf
We combine a robust, reliable UV (Visible/Ultraviolet
absorbance detector) monitor with a specialized
wireless tablet optimized for use in sensitive*
environments. The Biotage UV Monitor kit features
the latest in fiber optic technology and a powerful
SW package to provide the most effective and
flexible UV monitoring system for Biotage® Flash 150
and Biotage® Flash 400 purification systems.
Part No: AN117-HORIssued year: 2017File size: 4.2mbFile type: pdf
The US EPA monitors a variety of chemicals in water that may cause harm to humans or wildlife to minimize exposure. Method 625 was developed by the Office of Science and Technology in the Clean Water program to monitor a large suite of semivolatile chemicals in wastewater for compliance with the National Pollution Discharge Elimination System (NPDES).
Part No: PPS525Issued year: 2017File size: 0.19mbFile type: pdf
Safety is always a prime consideration in a laboratory. While working with organic solvents and samples that may contain harmful analytes, it makes sense to shield the operator from hazardous conditions whenever possible.
Part No: PPS468Issued year: 2017File size: 0.71mbFile type: pdf
Producing peptides for research is challenging. Biotage has developed
a holistic approach to the entire peptide workflow via an automated solution, designed for dedicated peptide researchers and those new to the field.
Part No: PPS466.GermanIssued year: 2017File size: 0.41mbFile type: pdf
Modelle haben gezeigt, dass sich die zur Herstellung
eines Zielmoleküls benötigte Zeit mit dem automatisierten
Workflow von Biotage um bis zu achtzig
Prozent verkürzen lässt, sodass auch die Projektlaufzeiten
entsprechend verkürzt werden können.
Part No: PPS463Issued year: 2017File size: 1.74mbFile type: pdf
Traditional approaches to compound purification involving chromatography utilize large volumes of relatively toxic and expensive solvents, and significantly contribute to the environmental footprint of organizations involved in molecular research. Current directives for greener chemistry have put pressure on organizations to reduce the
environmental impact of their work.
Part No: PPS463.gerIssued year: 2018File size: 0.68mbFile type: pdf
Bei herkömmlichen Aufreinigungsverfahren mittels Chromatographie kommen große Mengen verhältnismäßig giftiger und teurer Lösungsmittel zum Einsatz, die sich negativ auf den ökologischen Fußabdruck von Unternehmen in der Molekularforschung auswirken. Neue Richtlinien für einen verantwortungsvolleren Umgang mit Chemikalien zwingen Unternehmen, die Umweltauswirkungen ihrer Arbeit zu reduzieren. Durch Einblicke in die Arbeitsabläufe dieser Unternehmen und neue Entwicklungen im Bereich Flash-Chromatographiesysteme und -säulen lassen sich Verbrauch und Toxizität der für die Aufreinigung eingesetzten Lösungsmittel und somit auch die Umweltbelastungen dieser Verfahren reduzieren.
Part No: PPS466Issued year: 2017File size: 1.4mbFile type: pdf
Using the Biotage automated workflow, models have shown that the time taken to produce a target molecule can be reduced by up to eighty percent, allowing project delivery timescales to be reduced
Part No: PPS356Issued year: 2014File size: 1.19mbFile type: pdf
To save money on consumables, many chemists choose to reuse
silica flash cartridges. This is true but risks purification results because chromatographic separation performance will change from run to run which reduces purification quality, especially in normal phase systems.
Regardless of the cartridge brand used, repeated use of silica flash cartridges results in loss of compound resolution and therefore fraction purity.
Part No: TechTipIssued year: 2011File size: 0.07mbFile type: pdf
It’s important to replace these filters to ensure proper operation and protection of the internal solvent delivery valves. This filter not only traps particulates, but also protects the valves as particulates may get trapped within and could lead to improper solvent dispensing even when the extractors are at idle.
Part No: TN121Issued year: 2006File size: 0.07mbFile type: pdf
In medicinal chemistry reactions are often carried out in solvents such as DMSO. Once the reaction is complete, it is usually necessary to separate the products of the reaction from reagents and by-products. This can be performed using strong cation exchange sorbents (e.g. ISOLUTE SCX-2 or ISOLUTE Flash SCX-2) to selectively isolate the basic compounds from the reaction mixture. (see Figure 1 for structure of ISOLUTE SCX-2 and ISOLUTE Flash SCX-2 sorbents)
Part No: TN135.06Issued year: 2006File size: 0.09mbFile type: pdf
One of the most common work-up techniques in medicinal chemistry is liquid-liquid extraction (LLE) using a
separating funnel. The technique is used for removal of water soluble impurities, solvent exchange, water
removal or work-up of aqueous reaction mixtures.
Part No: TN135Issued year: 2006File size: 0.1mbFile type: pdf
One of the most common work-up techniques in medicinal chemistry is liquid-liquid extraction (LLE). The technique is used for removal of water soluble impurities, solvent exchange, water removal or work-up of aqueous reaction mixtures.
Part No: PPS352.v3Issued year: 2015File size: 7.34mbFile type: pdf
Biotage offers a range of products for reaction mixture work-up and organic sample preparation. The solutions include a range of techniques and also address throughput requirements from filtration, catch & release, and scavenging cartridges in organic synthesis workflows.