Part No: AN067Issued year: 2012File size: 1.01mbFile type: pdf
La cromatografía flash en fase normal y fase reversa facilita el aislamiento de compuestos
polares presentes en extractos de productos naturales. La combinación de avanzadas funciones
de detección tales como barrido espectral, y evaporativo de luz dispersa (ELSD) incrementa la
efectividad de la cromatografía flash en procesos de purificación de productos naturales.
Part No: P169Issued year: 2017File size: 0.23mbFile type: pdf
Improvements in solid phase peptide synthesis strategies and
development of resin linkages susceptible to low acid cleavage
conditions has enabled synthesis of long peptides while keeping the
protecting groups intact. This strategy is now used for the
preparation of chemically synthesized proteins, wherein shorter
peptide fragments are ligated together. They are also found in the
synthesis of peptide macrocycles that utilize head-to-tail cyclization strategies. Although linear synthesis of protected peptides is generally straightforward, purification of these compounds using traditional reversed phase methods is quite challenging. Herein we describe the use of normal phase chromatography for purification of fully protected peptides.
Part No: AN052-HORIssued year: 2009File size: 0.72mbFile type: pdf
Effective removal of residual water from organic solvents is critical to achieve optimal recoveries for water soluble compounds. Sodium sulfate has been the standard technique for removing residual water from solvent extracts for many years; however, it has several major drawbacks. Water soluble analytes in the residual water phase can become adsorbed in the sodium sulfate, leading to lower recoveries.
Part No: AN886Issued year: 2017File size: 1mbFile type: pdf
This application note describes the extraction of 96 licit and illicit drugs of abuse from urine prior to UPLC-MS/MS analysis using EVOLUTE® HYDRO CX 96-well plates.
EVOLUTE® HYDRO CX plates offer an efficient way to perform hydrolysis in the well of the extraction plate. This method provides high analyte recovery, reduced extraction time due to the elimination of a sample transfer step as well as the elimination of the column conditioning and equilibration steps, and a reduced risk for sample carryover or cross-contamination due to the elimination of the sample transfer step.
Part No: AN863Issued year: 2016File size: 1.62mbFile type: pdf
This application note describes the use of ISOLUTE® ENV+ on-line cartridges in a fully automated on-line SPE-LC-MS/MS method for extraction and quantification of 16 acid herbicides in drinking and surface water.
Part No: SSI-LCMS-086Issued year: 2017File size: 0.37mbFile type: pdf
Catecholamines and Metanephrines were analyzed using a LCMS-8060 triple quadrupole mass spectrometer. Calibrators were prepared in synthetic urine and extracted using solid phase extraction. Extractions were performed using weak cation exchange on the EVOLUTE® EXPRESS WCX 10 mg 96-well plate from Biotage.
Part No: P164Issued year: 2017File size: 0.69mbFile type: pdf
Most drugs, both licit and illicit, are excreted in urine as glucuronide conjugates. Hydrolysis using beta-glucuronidase converts the glucuronidated metabolites back to their “free” or non-conjugated
form, increasing sensitivity for LC-MS/MS analysis. Hydrolysis using red abalone, abalone, and recombinant beta-glucuronidase enzymes was evaluated using an in-well hydrolysis plate to determine which provided the most efficient hydrolysis of glucuronide metabolites without affecting overall recovery of nonconjugated compounds. A glucuronide control containing 8 glucuronide compounds was used to determine the extent of hydrolysis that occurred. A non-conjugated control containing 96 licit and illicit drugs of abuse was evaluated to determine if signal suppression occurred as a result of enzyme hydrolysis.
Part No: AN103-HORIssued year: 2016File size: 1.61mbFile type: pdf
Pesticides, including insecticides, herbicides and fungicides are used extensively to increase agricultural yields. The total world usage of pesticides in 2007 was approximately 5.2 billion pounds with the US consuming approximately 22% of the total.1 The wide use of pesticides yields concern that drinking water sources will become contaminated, exposing the population to hazardous substances that may cause cancer.
Part No: P202Issued year: 2016File size: 0.59mbFile type: pdf
US EPA Method 1664 for Hexane Extractable Material (HEM) or Oil and Grease has allowed use of solid phase extraction (SPE) instead of liquid-liquid extraction (LLE) with hexane since 2007 and this has been widely adopted in the US. SPE is an equivalent extraction technique to LLE and produces the same n-hexane extract. The extract, similar to LLE, may contain residual water that must be treated properly and removed from the n-hexane extract.
Part No: SSI-LCMS-096Issued year: 2018File size: 0.87mbFile type: pdf
Analysis of the perfluorinated alkyl acids listed in EPA Method 537 along with fluorotelomeric alcohols and perfluorinated sulfonates were extracted using SPE (using ISOLUTE 101 500 mg/ 6 mL SDVB SPE columns) and analyzed on the LCMS 8045. Minimum Detection Limits(MDL) ranged from 0.69 to 3.25 ppt. Extraction recoveries were greater than 80% for all compounds, with surrogate recoveries within 10% of the true value.
Part No: AN080-HORIssued year: 2015File size: 1.18mbFile type: pdf
This application note demonstrates the use of automated solid phase extraction for a variety of pharmaceutical compounds in drinking water,
wastewater and surface water. The criteria specified in US EPA Method 1694 was met, even at very low concentrations. For lower concentrations a larger sample of water may be processed.
Part No: AN862Issued year: 2016File size: 1.48mbFile type: pdf
This application note describes the use of EVOLUTE® EXPRESS ABN on-line cartridges in a fully automated on-line SPE-LC-MS/MS method for extraction and quantification of 13 commonly prescribed pharmaceuticals (with a range of characteristics) in water.