Part No: AN818.v1Issued year: 2014File size: 1.54mbFile type: pdf
This application note describes the extraction of ethyl glucuronide and ethyl sulfate from human urine using ISOLUTE® NH2 solid phase extraction columns. The method has been applied real patient samples that had been previously analyzed with a validated referee method. The results of the orthogonal measurements agreed, to provide similar diagnostic values.
Part No: P144Issued year: 2016File size: 0.6mbFile type: pdf
The ability to extract a broad range of different drugs from a biological matrix allows for the expedited analysis of a patient sample using LC-MS/MS. Typically small molecules are extracted from matrices like urine based on their polarities. A fast and reliable sample preparation method that could be implemented to extract drugs of different polarities from urine could be used as a screening tool to quickly identify the presence of illicit drugs in patient samples using LC-MS-MS.
This poster demonstrates the utility of supported liquid extraction for the extraction of over 30 different acidic, basic and neutral drugs in urine prior to LC-MS/MS.
Part No: AN944Issued year: 2020File size: 1.86mbFile type: pdf
The development of a novel high-pressure liquid chromatography/ triple quad mass spectrometry method allows for the efficient and accurate identification of 12 synthetic cannabinoids in whole blood by LC-MS/MS. Samples are fortified with internal standard, buffered, and extracted using Supported Liquid Extraction (SLE).
Part No: AN099-HORIssued year: 2014File size: 2.06mbFile type: pdf
Aflatoxins, a mold largely produced by Aspergillus flavus and Aspergillus parasiticus1 are commonly tested mycotoxins found naturally in a wide range of agriculture crops and food products. Due to their harmful effects on human health, animal health, and global trade, aflatoxins are regulated in most countries and have established global limits in a wide variety of matrices.
Part No: P035Issued year: 2012File size: 0.26mbFile type: pdf
Vitamin D deficiency can result in various health issues such as osteoporosis, liver and kidney problems and is associated with increased risk of cancers and multiple
sclerosis. From this standpoint vitamin D analysis has extremely important clinical relevance. This poster presents a novel method for the extraction of 25 hydroxyvitamin D demonstrating acceptable validation parameters from serum calibrators and DEQAS supplied samples using ISOLUTE SLE+.
Part No: P109Issued year: 2014File size: 0.43mbFile type: pdf
This poster describes a supported liquid extraction method for extraction of a class of novel psychoactive substances (NBOMes) with analysis using laser diode thermal desorption (LDTD) MS/MS. Samples are oral fluid collected using Salivettes.
Part No: P081Issued year: 2014File size: 0.42mbFile type: pdf
This poster describes a reduced workflow solid phase extraction method for extraction of vitamin B7 (biotin) from human serum. Using EVOLUTE EXPRESS AX 96-well plates, the traditional sorbent conditioning and equilibration steps are not required, meaning the sample can be applied directly to the dry plate. Post extraction evaporation is also eliminated.
Part No: P091Issued year: 2014File size: 0.38mbFile type: pdf
This report details a “load, wash, elute” weak cation exchange solid phase extraction procedure amenable to both plasma and urine samples. The extracts are subsequently injected into an LC-MS/MS system. The preliminary sample preparation method was developed at the Biotage US Applications lab (Charlotte, NC).The method was then transferred to Ionics (Bolton, ON, Canada) to facilitate the nmole/L measurements of the selected biomarkers
by laminar flow tandem mass spectrometry. The SPE-LC-ESIMS/ MS method parameters were first optimized using pooled mixed gender plasma. A set of patient samples (n=32) was later supplied by the Mayo Clinic (Rochester, MN) that had previously been analyzed by a validated referee method. The population represented measured values across a range of clinical relevance.
Part No: AN111-HORIssued year: 2016File size: 2.03mbFile type: pdf
The compound 4-methylimidazole (4-MEI) is formed as a byproduct in some foods and beverages. Caramel coloring Type III and Type IV in beverages is one of the ingredients which may contain 4-MEI. Products that potentially contain 4-MEI include certain colas, beers, soy sauces, breads, coffee, ammoniated livestock feed and other products.
Part No: P183Issued year: 2018File size: 1.3mbFile type: pdf
The compound 4 Methylimidazole (4-MeI) is made during the production of caramel color commonly found in foods and beverages, especially sodas.
The Codex Alimentarius of the World Health Organization sets a limit of 250 mg/kg of 4-MeI. Also, the state of California has added 4-MeI to its Prop 65 list at an exposure level of 29 μg/day.
This study looks at 4-MeI levels in sodas and malt sodas which
are sold in and outside the state of California. The first section, included in this poster, describes the performance of automated solid
phase extraction of 4-MeI using conventional HPLC/MS detection.
ASMS 2018, San Diego, CA
Part No: UI425Issued year: 2019File size: 0.3mbFile type: pdf
The newly released Biotage® Selekt flash chromatography instrument can be run
at a maximum flowrate of 300 mL/min or a maximum pressure of 30 bar. These
high flowrates and pressures enable a user to perform chromatography using
not only dry-packed, single-use plastic flash columns containing small (≥20 μm)
spherical silica particles, but also semi-preparative, slurry-packed HPLC columns
for multiple use with smaller (≤20 μm) spherical silica particles.
Part No: P044Issued year: 2012File size: 0.89mbFile type: pdf
The objective was to develop a GC-MS assay for the determination of free benzodiazepines using Supported Liquid Extraction (SLE). The SLE extraction mechanism is very efficient, delivering higher analyte recoveries and cleaner extracts than equivalent LLE methods.
ISOLUTE, SLE, SLE+, Supported Liquid Extraction, Benzodiazepines, Forensic, Drugs, DOA, Drugs of Abuse, SOFT,
Part No: P198.V.1Issued year: 2019File size: 0.59mbFile type: pdf
The low-level detection required for cannabis use combined with the complexity of hair testing makes for a challenging application. This poster aims to demonstrate simplified sample preparation workflow for low level analysis of THC and metabolites from hair, using Biotage Lysera and ISOLUTE SLE+ columns for sample preparation.
Part No: Issued year: 2016File size: 0.94mbFile type: pdf
Cortisol measurement in hair samples scan be a useful indicator of chronic stress. This poster compares a new LC-MS/MS method with an existing immunoassay based method.
The Biotage Bead Ruptor is used effectively for hair pre-treatment.
Part No: AN051-HORIssued year: 2009File size: 0.65mbFile type: pdf
The DryDisk® Separation Membrane was designed to replace conventional sodium sulfate in the drying of sample extracts. The DryDisk® is a hydrophobic membrane, consisting of a GORETEX ® process filtration media based on polytetrafluroethylene (PTFE). The hydrophobic property of the membrane allows the extracting solvent to pass through it, while retaining any water in the sample extract on the membrane’s surface.
Part No: AN098-HORIssued year: 2014File size: 2.32mbFile type: pdf
Mycotoxin testing awareness has increased as countries involved in world trade of raw agriculture and processed consumer products rely on a safe global food supply. Several mycotoxins are naturally produced and monitored; however, aflatoxins, produced mainly by the Aspergillus species, are considered to be the most important mycotoxin group in the world’s food supply to monitor.
Part No: PPS571Issued year: 2019File size: 0.61mbFile type: pdf
On one of the top floors in the Baylor College of Medicine Neurological Research Institute, I visited the Center for Drug Discovery and learn from the Director and Medicinal Chemistry Leader, Dr. Justin Anglin, about muscle cachexia as well as their innovative projects focused
on male contraceptives. We were able to take a tour of their state-of-the-art lab after which includes a full suite of Biotage products, something Dr. Anglin uses every day.
Part No: PPS498Issued year: 2018File size: 0.9mbFile type: pdf
PeptiDream Inc. relocated their head office in August 2017 and established its new research
center in the Kawasaki City International Strategic Center. The expanded facility is expected
to spawn innovative new drugs. The base instrumentation is provided by Biotage. R&D
Director Keichi Masuya and his staff elaborate on the development.
Part No: PPS615Issued year: 2020File size: 0.52mbFile type: pdf
Recently, the Thorn-Seshold Research Group at
Ludwig Maximilian University of Munich (LMU)
purchased a Biotage® Selekt flash purification
system to help them with their work. Biotage caught
up with two of the researchers, Markus Müller and Li
Gao, both Ph.D. students, to hear about their experiences
with the system.