Initiator+ Alstra Testimonial Dr Longhi

Customer Cases English

On a crisp December day, I travelled up to Queens, New York to speak with Dr. Aaron Muth from St. John’s University about his work in cancer research with Biotage lab equipment and specifically the SNAP Bio cartridges. As an Assistant Professor of Medicinal Chemistry, Dr. Muth and the members of his research group use our equipment every day. We chatted about his research, students, and the needs related to tools and equipment.

Customer Cases

VacMaster Disk Getting Started Guide (User Manual)

English Environmental Manuals & User Guides Petroleum

Connecting Flash cartridge to Biotage Flash systems

English Manuals & User Guides

This poster compares various sample preparation techniques (mixed-mode SPE (EVOLUTE EXPRESS CX, phospholipid depletion (ISOLUTE PLD+), supported liquid extraction (ISOLUTE SLE+) and dual mode extraction (ISOLUTE HYDRO DME+) for extraction of a panel of 98 drugs of abuse from urine. SOFT 2019

96-well plate Amphetamines Anticonvulsant Antipsychotics Barbiturates Benzodiazepines Cannabinoid Clinical English Fentanyls Forensic Hallucinogen LC-MS/MS Opiates Opioid Posters Stimulants z-drugs

This poster compares solid phase extraction (EVOLUTE EXPRESS CX and ISOLUTE HCX) with supported liquid extraction (ISOLUTE SLE+) for extraction of fentanyl analogues from whole blood samples. NEAFS 2019

4-ANPP (4-aminophenyl-1-phenethylpiperidine) 4-fluoro-isobutyryl-fentanyl 96-well plate Acryl fentanyl Alfentanil Butyryl fentanyl Carfentanil Clinical English Fentanyl Fentanyls Forensic Furanyl fentanyl Isobutryl fentanyl Methoxyacetyl fentanyl Norfentanyl Opioid Posters Sufentanil U-47700 U-51754 Valeryl fentanyl Whole blood

Corrosion costs the petroleum industry an estimated $1.3 billion in non-productive time, materials and labor annually. Imidazolines prepared from fatty acids and amines are a widely-used class of chemical corrosion inhibitor, due to excellent performance and ease of handling. However, commercial imidazolines are actually mixtures of several different chemical compounds, and the relative proportions of these species can have a large impact on both corrosion inhibition and product physical properties. The absence of gold standard analytical methods to characterize the active ingredients in imidazoline formulations limits the understanding of the chemistry of these materials. It is for this reason that a SPE-LC-MS method was developed to supplement the chemical information afforded by bulk testing / wet chemistry methods. It is anticipated that this method will have significant impact in the formulation of new corrosion inhibitors for oilfield applications as well as the quality control of finished products in manufacturing. GCC 2014

Column Corrosion inhibitors English Imidazoline corrosion inhibitor Imidazolines LC-MS TOF Petroleum Posters

This study shows that the SPE-DEX® 4790 Automated Extractor system can process water samples containing suspended particulate matter, up to 1000 mg, for the analysis of PAH’s. As PAH’s are found in both the dissolved and particulate phase of the water sample, it is important to be able to handle “whole” water samples such that the particulate matter in the water sample is retained and extracted with the SPE disk. This filtration method ensures that the PAH’s found in the dissolved phase, and those adsorbed onto the particulate matter are properly extracted.

Acenaphthene Acenapthalene Anthracene Application Notes Benzo (g,h,i) perylene Benzo(a)anthracene Benzo(a)pyrene Benzo(b)fluoranthene Benzo(k)Fluoranthene Chrysene Dibenzo(ah)anthracene Disk English Environmental Fluoranthene Fluorene GC-MS Indeno(123-cd)pyrene PAHs Persistant organic pollutants (POP) Phenanthrene Polyaromatic Hydrocarbons (PAH) Pyrene

This poster details current sample preparation strategies for aminoglycoside antibiotics in meat developed in a collaborative effort with Biotage and Tyson Foods, Inc. Springdale Corporate Laboratory to optimize analyte recovery (>70%), minimize analyte suppression and maintain acceptable method precision. FAST LC-MS 2014

Aminoglycoside antibiotics Chicken meat Column English Food Gentamicin LC-MS/MS Neomycin Posters Spectinomycin Streptomycin Veterinary drugs

Posters

This poster describes a strong cation exchange SPE protocol for extraction of Vitamin B3 (Niacin) and related metabolites from serum. The ISOLUTE SCX-3 96 well plate format demonstrated as a viable option for serum measurements over a relevant concentration range in clinical diagnostics. It is anticipated that strategies presented in this poster would be of broad-based interest to clinical laboratories focused on combining parent/metabolite assays into a single analysis. MSACL 2014

96-well plate B Vitamins Clinical English LC-MS/MS Niacin (nicotinic acid) Niacinamide Nicotinuric acid Posters Serum Vitamin B3 Vitamins

This application note describes the extraction of Vitamin B3 (Niacin, nicotinic acid) and its key metabolites from serum using a strong cation exchange SPE retention mechanism. High reproducible analyte recoveries are achieved.

96-well plate Application Notes B Vitamins Clinical English LC-MS/MS Niacin (nicotinic acid) Niacinamide Nicotinuric acid Serum Vitamin B3 Vitamins

This application note describes the extraction of nine phthalate metabolites from human urine using ISOLUTE® ENV+ solid phase extraction columns.

Application Notes Biomarker Clinical Column English LC-MS/MS Mono(2-ethyl-5-carboxypentyl) phthalate (MECPP) Mono(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP) Mono(2-ethylhexyl) phthalate (MEHP) Monobenzyl phthalate (MBzP) Monobutyl phthalate (MBP) Monoethyl phthalate (MEP) Monohexyl phthalate (MHxP) Monoisononyl phthalate (MiNP) Monomethyl phthalate (MMP) Phthalate Metabolites Phthalates Urine

I got to take a tour of the undergraduate lab at California State University, San Marcos with Dr. Robert Lafe. His lab is one of the first to have the new Biotage® Selekt Flash Purification System paired with the Sfär columns. We discussed how his undergraduate researchers were impacted by the broadened capabilities and their opportunity to learn about new instruments, and how it has affected his own research in his goals to gain tenure at the university.

Customer Cases

Pain management therapy warrants constant monitoring of therapeutic levels of prescribed drug levels in patient urine samples. The number of samples being submitted for analysis has increased dramatically in the last 10 years with improvements in high throughput automated screening capabilities. Patient samples analysis is complicated by the need for an effective sample preparation methodology that can extract target analytes from complex matrices with good efficiency. Further complicating the process is the need to enzymatically hydrolyse the glucoronidated metabolites prior to extraction from the urine matrix. A fully automated sample preparation process using a TECAN Freedom EVO® 100 was designed to incorporate both the enzymatic hydrolysis and subsequent sample preparation assay as one continuous workflow. Supported Liquid Extraction (ISOLUTE SLE+) which offers an efficient alternative to traditional liquid-liquid extraction (LLE) and solid phase extraction (SPE) techniques was used to extract a suite of pain management drugs from spiked urine samples. A recovery and quantitation assay was run on the TECAN Freedom EVO® 100 using mock patient samples to demonstrate utility of automation process. MSACL, Pain Management, Biotage, SPE, SLE, LLE, Supported Liquid Extraction, Drugs, MSACL, San Diego, 2013

6-acetylmorphine (MAM) 96-well plate Benzodiazepines Clinical Clonazepam Codeine Diazepam Dihydrocodeine (DHC) Drugs of Abuse Flunitrazepam Hydrocodone Hydrolysed urine Hydromorphone LC-MS/MS Methadone Methamphetamine Morphine Nitrazepam Opiates Oxycodone Oxymorphone Posters Urine

This poster presents the synthesis of peptidoglycan fragments where the coupling of peptides and carbohydrates was achieved using microwave heating. The fully automated total synthesis of a complex branched peptide is also presented. The fragments were synthesized using Biotage® Initiator+ SP Wave, Biotage® Syro Wave™ and Biotage® Initiator+ Alstra™ respectively. Presented at EPS, Sofia, 2014.

Posters

The potential harm caused by synthetic cannabinoids is a significant concern since exposures and reports of human fatalities due to these substances is widespread [5]. Detection of these relatively new type designer drugs in blood is essential in forensic toxicology, other scientific and medical fields. Cannabinoids, SLE, SLE+, Supported Liquid Extraction,

CP47,497 CP47,497-C8 Column Forensic HU210 JWH-018 JWH-073 JWH-200 JWH-250 JWH019 LC-MS/MS Posters Synthetic Cannabinoids (SPICE) Whole blood

This application note describes the extraction of ethyl glucuronide and ethyl sulfate from human urine using ISOLUTE® NH2 solid phase extraction columns. The method has been applied real patient samples that had been previously analyzed with a validated referee method. The results of the orthogonal measurements agreed, to provide similar diagnostic values.

Alcohol metabolite Application Notes Clinical Column Ethyl Sulfate (EtS) Ethyl glucuronide (EtG) Forensic LC-MS/MS Urine

The ability to extract a broad range of different drugs from a biological matrix allows for the expedited analysis of a patient sample using LC-MS/MS. Typically small molecules are extracted from matrices like urine based on their polarities. A fast and reliable sample preparation method that could be implemented to extract drugs of different polarities from urine could be used as a screening tool to quickly identify the presence of illicit drugs in patient samples using LC-MS-MS. This poster demonstrates the utility of supported liquid extraction for the extraction of over 30 different acidic, basic and neutral drugs in urine prior to LC-MS/MS. MSACL 2016

11-hydroxy- Δ9-tetrahydrocannabinol (THC-OH) 11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH) 6-acetyl codeine 96-well plate Acidic Alprazolam Amphetamine Barbiturates Basic Benzodiazepines Benzoylecgonine Buprenorphine Butalbital Clinical Clonazepam Codeine Dextromethorphan Diazepam Drugs of Abuse EDDP English Fentanyl Flunitrazepam Forensic Hydrocodone Hydrolysed urine Hydromorphone LC-MS/MS MDEA Morphine Naltrexone Neutral Nitrazepam Norbuprenorphine Norcodeine Norfentanyl Opiates Oxazepam Oxycodone Oxymorphone Pain Management Pentazocine Pentobarbital Phenobarbital Posters Propoxyphene Secobarbital THC THC metabolites THC-COOH Temazepam Δ9-tetrahydrocannabinol (THC)