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    PhyTips Featured In Journal Publications And Posters

    2023
    Structure elucidation of a human melanocortin-4 receptor specific orthosteric nanobody agonist
    Research Square Oct 2023
    Product used: IMAC PhyTip® Columns
    Discovery of neutralizing SARS-CoV-2 antibodies enriched in a unique antigen specific B cell cluster
    PLOS ONE Sep 2023
    Product used: Protein A PhyTip® Columns
    Examining SRP pathway function in mRNA localization to the endoplasmic reticulum
    RNA, Jul 2023
    Product used: C18 PhyTip Columns
    Detection of synthetic analogues of insulin-like growth factor 1 in different biological fluids by liquid chromatography quadrupole time-of-flight mass spectrometry: comparison of different immunoaffinity protocols
    Analytical and Bioanalytical Chemistry, Aug 2023
    Product used: Protein A PhyTip® Columns
    2022 White Paper on Recent Issues in Bioanalysis: Enzyme Assay Validation, BAV for Primary End Points, Vaccine Functional Assays, Cytometry in Tissue, LBA in Rare Matrices, Complex NAb Assays, Spectral Cytometry, Endogenous Analytes, Extracellular Vesicles Part 2 – Recommendations on Biomarkers/CDx, Flow Cytometry, Ligand-Binding Assays Development & Validation; Emerging Technologies; Critical Reagents Deep Characterization
     Bioanalysis, Aug 2023
    Product used:  Streptavidin PhyTip Columns
    High-Throughput and Format-Agnostic Mispairing Assay for Multispecific Antibodies Using Intact Mass Spectrometry
     Analytical Chemistry, Jun 2023
    Product used: ProPlus PhyTip® Columns
    N-glycans from serum IgG and total serum glycoproteins specific for endometriosis
    Nature Scientific Reports, Jun 2023
    Product used: Protein G PhyTip® Columns
    Large libraries of single-chain trimer peptide-MHCs enable antigen-specific CD8+ T cell discovery and analysis
    Communications Biology, May 2023
    Product used: IMAC PhyTip® Columns
    Efficient evolution of human antibodies from general protein language models
    Nature Biotechnology, April 2023
    Product used: ProPlus PhyTip® Columns
    Discovery of Nanomolar DCAF1 Small Molecule Ligands
    Journal of Medicinal Chemistry, March 2023
    Product used: IMAC PhyTip® Columns
    Streamlined process for automated plasmid purification reduces labour time and increases titres for AAV production
    KTH - Royal Institute of Technology, March 2023
    Product used: Biotage® PhyPrep System
    Neoantigen-targeted CD8+ T cell responses with PD-1 blockade therapy
    Nature, March 2023
    Product used: IMAC PhyTip® Columns
    An Integrated In Vivo/In Vitro Protein Production Platform for Site-Specific Antibody Drug Conjugates
    Bioengineering, February 2023
    Product used: ProPlus PhyTip® Columns, Protein A PhyTip® Columns
    An automated, low volume, and high-throughput analytical platform for aggregate quantitation from cell culture media
    Journal of Chromatography A, February 2023
    Product used: ProPlus PhyTip® Columns
    The post-translational modification profile 1 of TAR DNA-Binding Protein (TDP-43) in platelets of patients with Alzheimer's disease: An exploratory study for blood-based biomarker development
    bioRxiv, January 2023
    Product used: Protein A PhyTip® Columns
    Quantitative profiling of N-glycosylation of SARS-CoV-2 spike protein variants
    Glycobiology, February 2023
    Product used: Normal Phase PhyTip® Columns
    Stability and Requirement for Thiamin in a Cell Culture Feed Used to Produce New Biological Entities
    Cells, January 2023
    Product used: Protein A PhyTip® Columns
    2022
    Targeted degradation via direct 26S proteasome recruitment
    Nature, Dec 2022
    Product used: ProPlus PhyTip® Columns
    Non-viral precision T cell receptor replacement for personalized cell therapy
    Nature, Nov 2022
    Product used: IMAC PhyTip® Columns
    Distinct Glycosylation Responses to Spinal Cord Injury in Regenerative and Nonregenerative Models
    Journal of Proteome Research, May 2022
    Product used: Normal Phase PhyTip® Columns
    Autophagy and intracellular product degradation genes identified by systems biology analysis reduce aggregation of bispecific antibody in CHO cells
    New Biotechnology, May 2022
    Product used: ProPlus PhyTip® Columns
    Epigenetic comparison of CHO hosts and clones reveals divergent methylation and transcription patterns across lineages
    Biotechnology and Bioengineering, Apr 2022
    Product used: ProPlus PhyTip® Columns
    Loss of the intracellular enzyme QPCTL limits chemokine function and reshapes myeloid infiltration to augment tumor immunity
    Nature, Mar 2022
    Product used: Streptavidin PhyTip® Columns
    Structure-guided unlocking of NaX reveals a non-selective tetrodotoxin-sensitive cation channel
    Nature, Mar 2022
    Product used: C18 PhyTip Columns
    Phosphoproteome Profiling of the Receptor Tyrosine Kinase MuSK Identifies Tyrosine Phosphorylation of Rab GTPases
    Molecular & Cellular Proteomics, Feb 2022
    Product used: C18 PhyTip Columns
    Rapid discovery of diverse neutralizing SARS-CoV-2 antibodies from large-scale synthetic phage libraries
    mAbs, Jan 2022
    Product used: Protein A PhyTip® Columns
    2021
    Integrated workflow for urinary prostate specific antigen N-glycosylation analysis using sdAb partitioning and downstream capillary electrophoresis separation
    Analytica Chimica Acta, Nov 2021
    Product used: IMAC PhyTip® Columns
    Antibody toolkit reveals N-terminally ubiquitinated substrates of UBE2W
    Nature, Jul 2021
    Product used: ProPlus PhyTip® Columns
    An end-to-end automated platform process for high-throughput engineering of next-generation multi-specific antibody therapeutics
    mAbs, Apr 2021
    Product used: Protein A PhyTip® Columns, IMAC PhyTip® Columns, Miniprep PhyTip Columns
    Functional GLP-1R antibodies identified from a synthetic GPCR-focused library demonstrate potent blood glucose control
    mAbs, Mar 2021
    Product used: Protein A PhyTip® Columns
    PD-L1+ neutrophils contribute to injury-induced infection susceptibility
    SCIENCE ADVANCES, Mar 2021
    Product used: ProPlus PhyTip® Columns, CaptureSelect PhyTip Columns
    Activity of murine surrogate antibodies for durvalumab and tremelimumab lacking effector function and the ability to deplete regulatory T cells in mouse models of cancer
    mAbs, Jan 2021
    Product used: ProPlus PhyTip® Columns, CaptureSelect PhyTip Columns
    2020
    High-throughput, parallelized and automated protein purification for therapeutic antibody development
    Approaches to the Purification, Analysis and Characterization of Antibody-Based Therapeutics, 2020
    Product used: Protein A PhyTip® Columns
    Concurrent transfection of randomized transgene configurations into targeted integration CHO host is an advantageous and cost-effective method for expression of complex molecules
    Biotechnology Journal, Mar 2021
    Product used: Protein A PhyTip® Columns
    2019
    Association of an HDL Apolipoproteomic Score With Coronary Atherosclerosis and Cardiovascular Death
    Journal of the American College of Cardiology, May 2019
    Product used: PhyTip Columns with custom resins
    Semiautomated Small-Scale Purification Method for High-Throughput Expression Analysis of Recombinant Proteins
    High-Throughput Protein Production and Purification, Jul 2019
    Product used: ProPlus PhyTip® Columns
    High-Throughput Protein Production and Purification Methods and Protocols
    High-Throughput Protein Production and Purification Book, Jul 2019
    Product used: ProPlus PhyTip® Columns
    Perfusion cell culture for the production of conjugated recombinant fusion proteins reduces clipping and quality heterogeneity compared to batch-mode processes
    Journal of Biotechnology, Aug 2019
    Product used: PhyTip Columns with custom resins
    Salivary N-glycosylation as a biomarker of oral cancer: A pilot study
    Shedding Light into the Subcutis: A Mass Spectrometry Based Immunocapture Assay Enabling Full Characterization of Therapeutic Antibodies after Injection in Vivo
    DNA-Encoded Macrocyclic Peptide Library
    Direct imaging of the recruitment and phosphorylation of S6K1 in the mTORC1 pathway in living cells
    2018
    Impact of cell culture media additives on IgG glycosylation produced in Chinese hamster ovary cells
    Quantitative Assessment of Affinity Selection Performance by Using DNA-Encoded Chemical Libraries
    Development of a Selection Method for Discovering Irreversible (Covalent) Binders from a DNA-Encoded Library
    Automated high throughput microscale antibody purification workflows for accelerating antibody discovery
    Pyruvate Kinase Muscle-1 Expression Appears to Drive Lactogenic Behavior in CHO Cell Lines, Triggering Lower Viability and Productivity: A Case Study
    Critical considerations for immunocapture enrichment LC-MS bioanalysis of protein therapeutics and biomarkers
    Development of a shake tube-based scale-down model for perfusion cultures
    Conservation of oncofetal antigens on human embryonic stem cells enables discovery of monoclonal antibodies against cancer
    Improved Performance in Mammalian Cell Perfusion Cultures by Growth Inhibition
    Structure-Guided Combinatorial Engineering Facilitates Affinity and Specificity Optimization of Anti-CD81 Antibodies
    Multi-step high-throughput conjugation platform for the development of antibody-drug conjugates
    Design and Application of a DNA-Encoded Macrocyclic Peptide Library
    Controlling the Glycosylation Profile in mAbs Using Time-Dependent Media Supplementation
    Agonists and Antagonists of Protease-Activated Receptor 2 Discovered within a DNA-Encoded Chemical Library Using Mutational Stabilization of the Target
    Randomness in DNA Encoded Library Selection Data Can Be Modeled for More Reliable Enrichment Calculation
    2017
    Use of a protein engineering strategy to overcome limitations in the production of "Difficult to Express" recombinant proteins
    Auto-induction of Pichia pastoris AOX1 promoter for membrane protein expression
    Streamlining workflow and automation to accelerate laboratory scale protein production
    The Tetrameric Plant Lectin BanLec Neutralizes HIV through Bidentate Binding to Specific Viral Glycans
    Modulation and modeling of monoclonal antibody N-linked glycosylation in mammalian cell perfusion reactors
    Characterization of H type 1 and type 1 N-acetyllactosamine glycan epitopes on ovarian cancer specifically recognized by the anti-glycan monoclonal antibody mAb-A4
    Capillary electrophoresis analysis of N-glycosylation changes of serum paraproteins in multiple myeloma
    Excess reactive oxygen species production mediates monoclonal antibody-induced human embryonic stem cell death via oncosis
    Discovery of a Potent BTK Inhibitor with a Novel Binding Mode by Using Parallel Selections with a DNA-Encoded Chemical Library
    Quantitative Glycomics: A Combined Analytical and Bioinformatics Approach
    2016
    Efficient Qualitative and Quantitative Determination of Antigen-induced Immune Responses
    CP40 from Corynebacterium pseudotuberculosis is an endo-β-N-acetylglucosaminidase
    Twoplex 12/13 C6 aniline stable isotope and linkage-specific sialic acid labeling 2D-LC-MS workflow for quantitative N-glycomics
    Discovery of cofactor-specific, bactericidal Mycobacterium tuberculosis InhA inhibitors using DNA-encoded library technology
    Robust factor selection in early cell culture process development for the production of a biosimilar monoclonal antibody
    Process performance and product quality in an integrated continuous antibody production process
    Identifying a robust design space for glycosylation during monoclonal antibody production
    EndoSd: an IgG glycan hydrolyzing enzyme in Streptococcus dysgalactiae subspecies dysgalactiae
    High-throughput analysis of immunoglobulin G glycosylation
    Downstream process development strategies for effective bioprocesses: Trends, progress, and combinatorial approaches
    Rapid N-glycan release from glycoproteins using immobilized PNGase F microcolumns
    Implementation of an Automated High-Throughput Plasmid DNA Production Pipeline
    From haystack to needle: finding value with DNA encoded library technology at GSK†
    Expression, purification, and characterization of recombinant human and murine milk fat globule-epidermal growth factor-factor 8
    Automated screening for small organic ligands using DNA-encoded chemical libraries
    The Type IV Pilus Assembly ATPase PilB of Myxococcus xanthus Interacts with the Inner Membrane Platform Protein PilC and the Nucleotide-binding Protein PilM
    Evaluating high-throughput scale-down chromatography platforms for increased process understanding
    A Combination of Structural and Empirical Analyses Delineates the Key Contacts Mediating Stability and Affinity Increases in an Optimized Biotherapeutic Single-chain Fv (scFv)
    Lessons learned in building high-throughput process development capabilities
    2015
    Current advances in the development of high-throughput purification strategies for the generation of therapeutic antibodies 
    DNA Encoded Library Selections and Insights Provided by Computational Simulations
    High-throughput purification tools for rapid upstream process development are interchangeable for biologics
    Improved assembly of bispecific antibodies from knob and hole half-antibodies
    Comparative glycoprofiling of HIV gp120 immunogens by capillary electrophoresis and MALDI mass spectrometry
    Encoded library technology screening of hepatitis C virus NS4B yields a small-molecule compound series with in vitro replicon activity
    Poly(N-vinylimidazole/ethylene glycol dimethacrylate) for the purification and isolation of phenolic acids
    Use of the site-specific retargeting jump-in platform cell line to support biologic drug discovery
    A simplified and robust protocol for immunoglobulin expression in Escherichia coli cell-free protein synthesis systems
    Sample preparation for N-glycosylation analysis of therapeutic monoclonal antibodies by electrophoresis
    Generation of potent mouse monoclonal antibodies to self-proteins using T-cell epitope "tags"
    Augmented Binary Substitution: Single-pass CDR germ-lining and stabilization of therapeutic antibodies
    Optimisation of a simple method to transiently transfect a CHO cell line in high-throughput and at large scale
    Structure of a highly acidic β-lactamase from the moderate halophile Chromohalobacter sp. 560 and the discovery of a Cs(+)-selective binding site
    Automated pipeline for rapid production and screening of HIV-specific monoclonal antibodies using pichia pastoris
    Identification of anti-tumour biologics using primary tumour models, 3-D phenotypic screening and image-based multi-parametric profiling
    Arginine methylation and citrullination of splicing factor proline- and glutamine-rich (SFPQ/PSF) regulates its association with mRNA
    2014
    Use of a protein engineering strategy to overcome limitations in the production of "Difficult to Express" recombinant proteins
    Auto-induction of Pichia pastoris AOX1 promoter for membrane protein expression
    Streamlining workflow and automation to accelerate laboratory scale protein production
    The Tetrameric Plant Lectin BanLec Neutralizes HIV through Bidentate Binding to Specific Viral Glycans
    Modulation and modeling of monoclonal antibody N-linked glycosylation in mammalian cell perfusion reactors
    Parallel experimental design and multivariate analysis provides efficient screening of cell culture media supplements to improve biosimilar product quality
    Characterization of H type 1 and type 1 N-acetyllactosamine glycan epitopes on ovarian cancer specifically recognized by the anti-glycan monoclonal antibody mAb-A4
    Capillary electrophoresis analysis of N-glycosylation changes of serum paraproteins in multiple myeloma
    Excess reactive oxygen species production mediates monoclonal antibody-induced human embryonic stem cell death via oncosis
    Discovery of a Potent BTK Inhibitor with a Novel Binding Mode by Using Parallel Selections with a DNA-Encoded Chemical Library
    Quantitative Glycomics: A Combined Analytical and Bioinformatics Approach
    2013
    Strategy for selecting disposable bags for cell culture media applications based on a root-cause investigation
    The impact of microcarrier culture optimization on the glycosylation profile of a monoclonal antibody
    Combining phenotypic and proteomic approaches to identify membrane targets in a 'triple negative' breast cancer cell type
    CDR-restricted engineering of native human scFvs creates highly stable and soluble bifunctional antibodies for subcutaneous delivery
    Automated sample preparation for CE-SDS
    Facile fabrication and instant application of miniaturized antibody-decorated affinity columns for higher-order structure and functional characterization of TRIM21 epitope peptides
    Strategy for selecting disposable bags for cell culture media applications based on a root-cause investigation
    The impact of microcarrier culture optimization on the glycosylation profile of a monoclonal antibody
    Combining phenotypic and proteomic approaches to identify membrane targets in a 'triple negative' breast cancer cell type
    CDR-restricted engineering of native human scFvs creates highly stable and soluble bifunctional antibodies for subcutaneous delivery
    2012
    High-throughput process development of chromatography steps: advantages and limitations of different formats used
    Method for recovery and immunoaffinity enrichment of membrane proteins illustrated with metastatic ovarian cancer tissues
    2010
    Chip-based CE for rapid separation of 8-aminopyrene-1,3,6-trisulfonic acid (APTS) derivatized glycans
    Mass spectrometric-based stable isotopic 2-aminobenzoic acid glycan mapping for rapid glycan screening of biotherapeutics
    Widening the bottleneck: increasing success in protein expression and purification
    2009
    RNA Purification and Analysis: Sample Preparation, Extraction, Chromatography
    Ion Chromatography, 4th, Completely Revised and Enlarged Edition
    Fluorescent isotope-coded affinity tag 2: peptide labeling and affinity capture
    Enrichment of Amadori products derived from the nonenzymatic glycation of proteins using microscale boronate affinity chromatography
    High-throughput immunoglobulin G N-glycan characterization using rapid resolution reverse-phase chromatography tandem mass spectrometry
    2008
    Fluorescent isotope-coded affinity tag (FCAT). I: Design and synthesis
    A simple high-throughput purification method for hit identification in protein screening
    Selection against undifferentiated human embryonic stem cells by a cytotoxic antibody recognizing podocalyxin-like protein-1
    Boronic acid lectin affinity chromatography (BLAC). 2. Affinity micropartitioning-mediated comparative glycosylation profiling
    Sample preparation for the analysis of complex carbohydrates by multicapillary gel electrophoresis with light-emitting diode induced fluorescence detection
    A microscale yeast cell disruption technique for integrated process development strategies
    2007
    Boronic acid-lectin affinity chromatography. 1. Simultaneous glycoprotein binding with selective or combined elution
    Automated sample preparation facilitated by PhyNexus MEA purification system for oligosaccharide mapping of glycoproteins
    An automated microscale chromatographic purification of virus-like particles as a strategy for process development
    2006
    High-throughput affinity ranking of antibodies using surface plasmon resonance microarrays
    Material-enhanced laser desorption/ionization (MELDI)--a new protein profiling tool utilizing specific carrier materials for time of flight mass spectrometric analysis
    2005
    First meeting on Developments in Protein Interaction Analysis (DiPIA 2005). August 28-31, 2005, PA, USA
    Striving for purity: advances in protein purification
    Protein purification: pure but not simple