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ISOLUTE-NH2 Selector
Normal phase flash chromatography is a very effective purification technique however, issues can arise with streaking, if the products being separated have basic character. ISOLUTE-NH2 silica is compatible with a wide range of mobile phases, from popular normal to reversed-phase options. The amino silica is covalently bonded to the stationary phase backbone, thus reduces the number and provides a shielding effect for amine substrates, yielding improved selectivity, peak shape, purity and recoveries from flash purification. Biotage supplies this silica pre-packed into purification cartridges for development and scale up in a range of different chemistry scenarios.
Use the cartridge selector below to quickly identify compatible cartridges for your purification platform and / or view available cartridges by size. Development and process development scale cartridges intended for use on Biotage® Selekt or our larger scale development platform Biotage® Isolera LS connect to the system via universal Luer-lock style connectors. Cartridges for our larger process development and scale up systems Biotage® Flash 75 / Flash 150 or Flash 400 systems require a compression module which is provided with the system, or available as a spare part.
For over 20 years Biotage has been at the forefront of enabling flash purification technologies. ISOLUTE®-NH2 is a complimentary powerful solution when the most demanding of purification challenges and need for diverse solvent choice, presents itself.
Moving from lab scale to large scale comes with a number of additional considerations. Biotage® Isolera LS, Flash 75, 150 and 400 systems are extremely tough, economically viable scale-up purification systems that have been developed for this purpose. With the Biotage approach, scaling up flash purification methods is straightforward. Any method developed using a Biotage lab scale cartridge can be transferred to a larger cartridge using the scale-up factors. Once the development cartridge is determined, the lab scale method can be replayed, providing peak elution profiles for the expected scale up run which are suitable for further optimization if needed. Furthermore, Isolera LS comes with software that can automatically create linear gradients from TLC Rf spots; and then automatically convert those linear gradients into step gradients for application on a larger scale. Each of the larger flash systems may be configured with either ‘M’ or ‘L’ cartridge capability to further increase its flexibility and range.
Key supplier to industry for over 20 years, used in numerous patents and cGMP processes.
Purification from grams in development through to multi kilogram quantities of product for pre-clinical and commercial production campaigns.
Stationary phase silica is clean, inert and deployed to support the most demanding of purification goals.
With lot control, an extensive QA / QC documentation package including Safety Data sheets, ISO 9001 / 14001 registration certification, BSE / TSE statement, shelf-life statements, retest guidance, storage recomendations and more, Biotage silicas are deisnged for industry and professional scale up campaigns.
