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ISOLUTE® 103 Concentration of Prep-LC Fractions Using Catch & Release
ISOLUTE® 103 is a hydroxylated polystyrene divinylbenzene co-polymer. The resin is used for the isolation of a wide range of compounds from prep LC fractions using catch & release protocol. The approach replaces the need for time-consuming evaporation of aqueous based fractions.
During catch & release the media bed "catches” the compound of interest. Rinsing the cartridge with solvent removes impurities, leaving the compound of interest on the cartridge for subsequent elution by an appropriate solvent. Biotage has a range of cartridges depending on whether the product to be recovered is acidic, basic or neutral. Applications include: purification, solvent switch, concentrating & capturing and HPLC fractions.
1. Cartridge equilibration (can be optional)
2. Sample loading
3. Cartridge washing (impurity elution, product is retained)
4. Product elution
It may also be possible to retain impurities in step 3, such that the cartridge becomes a one pass, one step purification system.
1. Choose ISOLUTE 103 column, 5 times the total sample mass
2.Perform column equilibration, at 10–50 mL/min
3. Dilute sample to max 30% organic content
4. Load onto ISOLUTE 103 column, allow to flow under gravity
5. Wash column
6. Release organic product using methanol
