Glutathione PhyTip columns are used for automated purification of GST-tagged antibodies and other recombinant proteins. The affinity resin used in these columns demonstrate high binding affinity and capacity for affinity purification of functional, recombinant GST-tagged proteins. The GST-tag (glutathione S-transferase) is a small (26 kDa) protein that is known to improve the solubility of the expressed recombinant protein.
Our Glutathione PhyTip columns comes with validated DFC protocols for purification of GST-tagged proteins that can be transferred to any automated liquid handling system. Our buffer kits contain all the buffers is needed for the purification protocol. Note that the proper use of Glutathione PhyTip columns require slow flow rates of 0.06 mL/min.
PhyNexus now part of Biotage developed the PhyTip column in 2002 and have supported researchers with automated protein purification ever since. PhyTip columns purified samples by repeated back-and-forth cycles - a process called dual flow chromatography - allowing complete binding interactions in each purification step.
Glutathione PhyTip columns continuously produce samples of >90% purity even in the presence of 40x excel BSA.
Glutathione PhyTip columns routinely generate purified samples with high sample recoveries.
PhyTip columns format is the perfect tool for parallel processing of samples in micro-scale in a high throughput process.
Our PhyTip columns are available for all major liquid handling systems, allowing their use on existing instrumentation you may already have in your lab!
Effective purification by PhyTip columns enables a full purification protocol of 96 samples in as little as 15 minutes.
When choosing a PhyTip® column for your purification process, the optimal resin bed volume of the column depends on the total amount of protein present in your samples. If you are working with very low protein concentrations of 10 μg/mL or lower, we recommend using a resin bed volume one or two sizes bigger than indicated below for an effective purification process.
We offer PhyTip® columns available for all major liquid handling systems.
If you are not sure which PhyTip® column will that work best in your protocol, our specialists are here to help you.
Part No. | Description | Pack Size | List Price | |
---|---|---|---|---|
PTV-92-20-04 | Box of 96 PhyTip® columns (200 µl volume) containing 20 µl of Glutathione affinity resin per column, Agilent | 96 | 714 USD | Add to quote/cart |
PTB-91-10-04 | Glutathione PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, Beckman | 96 | 668 USD | Add to quote/cart |
PTX-91-10-04 | Glutathione PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, DD/Biotix | 96 | 668 USD | Add to quote/cart |
PTH-91-10-04 | Glutathione PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, Hamilton | 96 | 668 USD | Add to quote/cart |
PTR-91-10-04 | Glutathione PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, MEA 2 | 96 | 668 USD | Add to quote/cart |
PTR-91-10-04 | Glutathione PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, MEA 2 | 96 | 668 USD | Add to quote/cart |
PTT-91-10-04 | Glutathione PhyTip® columns (1 mL volume) containing 10 µL affinity media per column, Tecan | 96 | 668 USD | Add to quote/cart |
PTB-91-16-04 | Glutathione PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, Beckman | 96 | 2288 USD | Add to quote/cart |
PTX-91-16-04 | Glutathione PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, DD/Biotix | 96 | 2288 USD | Add to quote/cart |
PTX-91-16-04 | Glutathione PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, DD/Biotix | 96 | 2288 USD | Add to quote/cart |
PTH-91-16-04 | Glutathione PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, Hamilton | 96 | 2288 USD | Add to quote/cart |
PTR-91-16-04 | Glutathione PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, MEA 2 | 96 | 2288 USD | Add to quote/cart |
PTR-91-16-04 | Glutathione PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, MEA 2 | 96 | 2288 USD | Add to quote/cart |
PTT-91-16-04 | Glutathione PhyTip® columns (1 mL volume) containing 160 µL affinity media per column, Tecan | 96 | 2288 USD | Add to quote/cart |
PTB-91-20-04 | Glutathione PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Beckman | 96 | 768 USD | Add to quote/cart |
PTX-91-20-04 | Glutathione PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, DD/Biotix | 96 | 768 USD | Add to quote/cart |
PTX-91-20-04 | Glutathione PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, DD/Biotix | 96 | 768 USD | Add to quote/cart |
PTH-91-20-04 | Glutathione PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Hamilton | 96 | 768 USD | Add to quote/cart |
PTR-91-20-04 | Glutathione PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, MEA 2 | 96 | 768 USD | Add to quote/cart |
PTR-91-20-04 | Glutathione PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, MEA 2 | 96 | 768 USD | Add to quote/cart |
PTT-91-20-04 | Glutathione PhyTip® columns (1 mL volume) containing 20 µL affinity media per column, Tecan | 96 | 768 USD | Add to quote/cart |
PTR-91-32-04 | Glutathione PhyTip® columns (1 mL volume) containing 320 µL affinity media per column, MEA 2 | 96 | 2926 USD | Add to quote/cart |
PTB-91-40-04 | Glutathione PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, Beckman | 96 | 1085 USD | Add to quote/cart |
PTX-91-40-04 | Glutathione PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, DD/Biotix | 96 | 1085 USD | Add to quote/cart |
PTX-91-40-04 | Glutathione PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, DD/Biotix | 96 | 1085 USD | Add to quote/cart |
PTH-91-40-04 | Glutathione PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, Hamilton | 96 | 1085 USD | Add to quote/cart |
PTR-91-40-04 | Glutathione PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, MEA 2 | 96 | 1085 USD | Add to quote/cart |
PTT-91-40-04 | Glutathione PhyTip® columns (1 mL volume) containing 40 µL affinity media per column, Tecan | 96 | 1085 USD | Add to quote/cart |
PTB-91-80-04 | Glutathione PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Beckman | 96 | 1719 USD | Add to quote/cart |
PTX-91-80-04 | Glutathione PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, DD/Biotix | 96 | 1719 USD | Add to quote/cart |
PTX-91-80-04 | Glutathione PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, DD/Biotix | 96 | 1719 USD | Add to quote/cart |
PTH-91-80-04 | Glutathione PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Hamilton | 96 | 1719 USD | Add to quote/cart |
PTR-91-80-04 | Glutathione PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, MEA 2 | 96 | 1719 USD | Add to quote/cart |
PTT-91-80-04 | Glutathione PhyTip® columns (1 mL volume) containing 80 µL affinity media per column, Tecan | 96 | 1719 USD | Add to quote/cart |
PTB-92-20-04 | Glutathione PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, Beckman | 96 | 768 USD | Add to quote/cart |
PTR-92-20-04 | Glutathione PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, MEA 2 | 96 | 768 USD | Add to quote/cart |
PTT-92-20-04 | Glutathione PhyTip® columns (200 µL volume) containing 20 µL affinity media per column, Tecan | 96 | 768 USD | Add to quote/cart |
PTB-92-05-04 | Glutathione PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, Beckman | 96 | 546 USD | Add to quote/cart |
PTR-92-05-04 | Glutathione PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, MEA 2 | 96 | 546 USD | Add to quote/cart |
PTT-92-05-04 | Glutathione PhyTip® columns (200 µL volume) containing 5 µL affinity media per column, Tecan | 96 | 546 USD | Add to quote/cart |
PTX-93-20-04 | Glutathione PhyTip® columns (300 µL volume) containing 20 µL affinity media per column, DD/Biotix | 96 | 768 USD | Add to quote/cart |
PTX-93-20-04 | Glutathione PhyTip® columns (300 µL volume) containing 20 µL affinity media per column, DD/Biotix | 96 | 768 USD | Add to quote/cart |
PTH-93-20-04 | Glutathione PhyTip® columns (300 µL volume) containing 20 µL affinity media per column, Hamilton | 96 | 768 USD | Add to quote/cart |
PTX-93-05-04 | Glutathione PhyTip® columns (300 µL volume) containing 5 µL affinity media per column, DD/Biotix | 96 | 546 USD | Add to quote/cart |
PTX-93-05-04 | Glutathione PhyTip® columns (300 µL volume) containing 5 µL affinity media per column, DD/Biotix | 96 | 546 USD | Add to quote/cart |
PTH-93-05-04 | Glutathione PhyTip® columns (300 µL volume) containing 5 µL affinity media per column, Hamilton | 96 | 546 USD | Add to quote/cart |
PTT-95-16-04 | Glutathione PhyTip® columns (500 µL volume) containing 160 µL affinity media per column, Tecan | 96 | 2288 USD | Add to quote/cart |
PTT-95-20-04 | Glutathione PhyTip® columns (500 µL volume) containing 20 µL affinity media per column, Tecan | 96 | 768 USD | Add to quote/cart |
PTT-95-40-04 | Glutathione PhyTip® columns (500 µL volume) containing 40 µL affinity media per column, Tecan | 96 | 1085 USD | Add to quote/cart |
PTT-95-80-04 | Glutathione PhyTip® columns (500 µL volume) containing 80 µL affinity media per column, Tecan | 96 | 1719 USD | Add to quote/cart |
Biotage is the Global Go-To Separations Company, supporting customers from drug discovery and development through to diagnostics and analytical testing with intelligent and sustainable workflow solutions. Our expertise and top-tier separation solutions play a key role in streamlining our customers’ workflows and improving their outcomes. Headquartered in Sweden, Biotage operates globally with 700 employees, serving over 80 countries. Our company is listed on NASDAQ Stockholm (BIOT).
Copyright © 2024 Biotage