ProPlus PhyTip® Affinity Columns - The Antibody Purification System that is the Best of Protein A and Protein G

The PhyTip® columns are innovative purification tools that radically simplify the capture, purification and enrichment of proteins from a variety of sources. Key to the success of these purification tools is the design of the mechanism to retain the affinity resin bed, with minimum dead volume and maximum capture potential. These Protein A and Protein G PhyTip columns specifically bind antibody (IgG) from different sources under certain optimal conditions, thus allowing nucleic acids and other contaminants to be removed. Following a rapid wash step, purified antibodies are easily eluted with commonly used low pH buffer. This technique allows for exceptionally high yields of IgG, depending on various conditions and provides for highly selective purification. PhyTip columns have extremely high binding capacity that can bind up to a few mgs of IgG, and can efficiently recover as little as 200 ng of IgG.