PhyTip® Columns Versus Other Common Technologies: A Comparative Analysis of Protein Purification Yields

This study focused on evaluating purified protein yields from PhyTip® columns against other common technologies, namely filter plates, spin columns and magnetic beads. PhyTip® columns containing ProPlus (MabSelect Sure) and Ni-IMAC resins were used to purify IgG spiked in CHO cells and His-eGFP in BL21 cells, respectively. Capacity testing showed that PhyTip® columns outperformed filter plates, spin columns, and magnetic beads, offering superior binding efficiency and higher yields across various protein concentrations. Besides yield, we have highlighted the ease of use, ease of automation, and process control benefits of PhyTip® columns, making them the product of choice for protein purification applications.