PhyTip® Columns Versus Other Common Technologies: A Comparative Analysis of Protein Purification Efficiency and Yield Consistency

PhyTip® columns were evaluated against other commonly used purification platforms for purifying recombinant proteins. The study assessed performance quality by examining yield consistency and protein purity using Ni-IMAC resin in PhyTip® columns, filter plates, magnetic beads and spin columns. PhyTip® columns outperformed filter plates, spin columns, and magnetic beads, providing higher yields with lower variability. The fully automated purification process ensured consistent recovery and batch-to-batch reproducibility. Additionally, the slow flow rates in PhyTip® columns prevented protein denaturation and aggregation, enabling efficient purification of small volume and low-titer samples.