Automated High-Throughput Parallel Peptide Purification

Peptide drug discovery has resulted in numerous globally approved drugs and many more in clinical development for treating diseases such as cancer, metabolic diseases, and cardiovascular diseases.

In the initial phases of drug discovery, high-throughput library synthesis is essential for preliminary screening, validation, and preliminary lead optimization. However, purification is a major bottleneck in producing peptide libraries. Reversed-phase
high-performance liquid chromatography (RP-HPLC) is the most common method for peptide purification, but it entails significant costs, in terms of both time and solvent usage. Due to the costs and throughput limitations of sequential purification, crude peptides are being evaluated in assays, which increases the risk of false-positive or false-negative results, thereby affecting the assay outcomes. To bridge the efficiency gap between peptide library synthesis and purification, Biotage offers two plate-based, automated parallel purification solutions, providing a range of purities suitable for various testing outcomes.