True Parallel Protein Purification with PhyTip® Columns Using the Freedom EVO® and MultiChannel Arm™ 96

PhyTip® columns are innovative purification tools that radically simplify the capture, purification and enrichment of proteins from a variety of sources. Key to the success of these purification tools is the design of the mechanism to retain the affinity resin bed, with minimum dead volume and maximum capture potential. Existing PhyNexus products include PhyTip columns affinity media for the purification of antibodies and tagged proteins, as well as conventional chromatography media for ion exchange and size exclusion chromatography, and hydrophobic separations.

Use of PhyTip columns, in conjunction with a Freedom EVO 100, 150 or 200 liquid handling platform equipped with a MultiChannel Arm™  96 (MCA 96) module, offers a high throughput automated solution for sample preparation and protein purification. For example, 96 hybridoma supernatants can be purified in as little as 15 minutes, thanks to the parallel pipetting capabilities of the MCA 96. The yield, purity and reliability of this procedure make it the ideal platform for sample preparation prior to high throughput applications such as antibody lead screening.