A Novel Approach to Automated High-Throughput Protein Enrichment and Characterization

The rapidly growing field of antibody and protein engineering continues to create the demand for technologies that increase the productivity and throughput of protein interaction analysis. We will describe a unique yet simple automated process to purify and enrich antibodies and proteins using PhyTip Columns and their subsequent quantification using a microfluidic LabChip device. Using this automated sample enrichment technique, proteins can be purified from complex samples, for example, the purification of IgGs from hybridoma cell culture supernatants.