Large Scale Microwave Heated SPPS of the ACP (65-74) Fragment Using Biotage® Initiator+ Alstra™ 662

The ability to synthesize peptides in a range of different scales is a requirement in many research laboratories. The Biotage® Initiator+ Alstra™ fully automated microwave peptide synthesizer has a scale range of 5 mmol up to 2 mmol using three different reactor vial sizes (5 mL, 10 mL and 30 mL). The acyl carrier protein fragment, ACP (65-74), H-Val-Gln- Ala-Ala-Ile-Asp-Tyr-Ile-Asn-Gly-NH2 (VQAAIDYING-NH2) (1), is a well-known so-called difficult sequence and is commonly used to evaluate the performance of new synthesis reagents and instrumentation. Here we demonstrate a variable scale synthesis on the Initiator+ Alstra using the ACP (65-74) fragment as a model peptide.